MRPS18A (NM_018135) Human Untagged Clone
CAT＃: SC113696
MRPS18A (untagged)-Human mitochondrial ribosomal protein S18A (MRPS18A), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 2400.00
CNY 2950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HumanS18b; MRP-S18-3; MRP-S18-a; MRPS18-3; S18bmt; S18mt-a |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113696 sequence for NM_018135 edited (data generated by NextGen Sequencing)
ATGGCGGCCCTCAAGGCTCTGGTGTCCGGCTGTGGGCGGCTTCTCCGTGGGCTACTAGCG GGCCCGGCAGCGACCAGCTGGTCTCGGCTTCCAGCTCGCGGGTTCAGGGAAGTGGTGGAG ACCCAAGAAGGGAAGACAACTATAATTGAAGGCCGTATCACAGCGACTCCCAAGGAGAGT CCAAATCCTCCTAACCCCTCTGGCCAGTGCCCCATCTGCCGTTGGAACCTGAAGCACAAG TATAACTATGACGATGTTCTGCTGCTTAGCCAGTTCATCCGGCCTCATGGAGGCATGCTG CCCCGAAAGATCACAGGCCTATGCCAGGAAGAACACCGCAAGATCGAGGAGTGTGTGAAG ATGGCCCACCGAGCAGGTCTATTACCAAATCACAGGCCTCGGCTTCCTGAAGGAGTTGTT CCGAAGAGCAAACCCCAACTCAACCGGTACCTGACGCGCTGGGCTCCTGGCTCCGTCAAG CCCATCTACAAAAAAGGCCCCCGCTGGAACAGGGTGCGCATGCCCGTGGGGTCACCCCTT CTGAGGGACAATGTCTGCTACTCAAGAACACCTTGGAAGCTGTATCACTGA Clone variation with respect to NM_018135.3 >OriGene 5' read for NM_018135 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGAAGAGGCGGCCCTCAAGGCTC TGGTGTCCGGCTGTGGGCGGCTTCTCCGTGGGCTACTAGCGGGCCCGGCAGCGACCAGCT GGTCTCGGCTTCCAGCTCGCGGGTTCAGGGAAGTGGTGGAGACCCAAGAAGGGAAGACAA CTATAATTGAAGGCCGTATCACAGCGACTCCCAAGGAGAGTCCAAATCCTCCTAACCCCT CTGGCCAGTGCCCCATCTGCCGTTGGAACCTGAAGCACAAGTATAACTATGACGATGTTC TGCTGCTTAGCCAGTTCATCCGGCCTCATGGAGGCATGCTGCCCCGAAAGATCACAGGCC TATGCCAGGAAGAACACCGCAAGATCGAGGAGTGTGTGAAGATGGCCCACCGAGCAGGTC TATTACCAAATCACAGGCCTCGGCTTCCTGAAGGAGTTGTTCCGAAGAGCAAACCCCAAC TCAACCGGTACCTGACGCGCTGGGCTCCTGGCTCCGTCAAGCCCATCTACAAAAAAGGCC CCCGCTGGAACAGGGTGCGCATGCCCGTGGGGTCACCCCTTCTGAGGGACANTGTCTGCT ACTCAAGAACACCTTGGAAGCTGTATCACTGACAGAGAGCAGTGCTTNCAGAGTTCCTTC TGCACCTGTGCTGGGGAGTANGAGGCCCACTCANCAGCCCTTTGGCCACACTATACTCCT GTCCCACCCCACACGATGGCCTGGTCCCTCCACATGCATGGACAGGNGACAGTGGGACTA ACTTCAGTACCCTTGGCCTGCACAGTANNCATGCTGGGAGCTANAGGCANGCAGGNCANN TGGNGTCCCTTNGCCAGCTGCTATGGGGCTTANGCCATGCTCAGTGCTGGGGACAAGGAN TTTTGCCCNACGCAGTGTCATAAACTGGGGTCATGGGNCTACCCATNGGNTGTGCGCTAC TGCTNGGAAGTGCGC >OriGene 3' read for NM_018135 unedited
GGTTGAAAGGCGCCGTANATGATTATCGAGTAGCCNNNNNNNNNNANANNAAATTTTTTT TTATATTTTTTTTTTTTTTATCTGTTTTTGTTTTTTTTTTCTTTTTTTGAAAAGATGGGA CCTGTTTGGCCCATGAATTCAATTGACTCATTGGCCCCATCAAAAAAAAACATGACTCAA TGGTTAACACCCAACTGGGAATTTTTCCAAAACCCCCTGGACTTTCCAAGGGGGGAGGGC CCCCCCAATGGGTAAACCCCTAAACCCCGTTTTTTACCCTGGGGTGGGGAAAAATCCTTT CCCCAGCACTGAAAATGGGCTAAACCCCAAAAAAGCTGGGAAGGGAACCAAATGGCCTGG CCGGCTTTAACTCCCAACATTGTTTCTTGGCCGGCCAAGGGTCCTGAAATTAATTCCACT GTCCCCTGTTCATGCCTGTTTGAGGGACCACACCCTTTTGGTGGGGGGGGACAGGAGTAT ATTTTTGACCCAAGGCTTTGTAGTGGGCCCCCCTCTCCCCACAAAGGTGCCGGAAAAACC CTTGAAACCCTGCTTTTTGTTCGAAATACAAATTCCAAGGGTTTTTTAGAAGAGAAATTT TTCCTTAAAAAGGGGGAACTCACGGGATTTCCCCCCCTTTTCTAAGGGGGGCCTTTTTTT TAAAGTGGTTTTATGGAACCCAAAACCCATAGCACAAGAAACTGGTGGGGTGTGGTTTAT TCTTACAACCACCCTTTCAAAAACCCCGGTCCTGATTTGTGAAAATAAAACTGGATGGGG GGCAATTTCAACACACCCGTGATATTTATGGGTCCTCTCGTGTAAGCCTGAGATCTTTTT GGAAGAAACTTCATGTAGCACGGTAAACGGTTTACACTCCAACACGCCTTATGTAAATTG CGCTCGGGCACCAGTGAGAAGGGCGCAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018135 |
| Insert Size | 1080 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018135.2, NP_060605.1 |
| RefSeq Size | 1060 bp |
| RefSeq ORF | 591 bp |
| Locus ID | 55168 |
| UniProt ID | Q9NVS2 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. A pseudogene corresponding to this gene is found on chromosome 3p. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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A Novel Technology for Multiplex Gene ExpressionAnalysis Directly from Whole Blood SamplesStabilized at Ambient Temperature Using anRNA-Stabilizing Buffer
,Buffer, RNAS;,
The Journal of Molecular Diagnostics
,PubMed ID 25684272
[MRPS18A]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203070 | MRPS18A (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S18A (MRPS18A), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 2400.00 |
|
| RC203070L3 | Lenti ORF clone of Human mitochondrial ribosomal protein S18A (MRPS18A), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC203070L4 | Lenti ORF clone of Human mitochondrial ribosomal protein S18A (MRPS18A), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG203070 | MRPS18A (tGFP-tagged) - Human mitochondrial ribosomal protein S18A (MRPS18A), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4370.00 |
|
| SC321075 | MRPS18A (untagged)-Human mitochondrial ribosomal protein S18A (MRPS18A), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 2400.00 |
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