BRF2 (NM_018310) Human Untagged Clone
CAT＃: SC113533
BRF2 (untagged)-Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2)
CNY 3656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BRFU; TFIIIB50 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113533 sequence for NM_018310 edited (data generated by NextGen Sequencing)
ATGCCAGGCAGAGGCCGCTGCCCGGACTGCGGCTCCACGGAGCTGGTGGAAGACTCGCAC TATTCGCAGAGCCAGCTGGTGTGCTCCGACTGCGGCTGCGTGGTCACCGAGGGGGTCCTT ACCACTACCTTCAGCGACGAGGGCAATCTCCGAGAGGTAACATATTCCCGAAGCACAGGG GAAAACGAACAAGTTAGTCGCAGCCAGCAACGAGGTCTCCGGCGAGTGAGAGACCTTTGT CGAGTTCTGCAGTTGCCACCAACATTTGAGGATACCGCGGTTGCCTACTACCAACAGGCA TATCGGCACTCTGGCATCCGAGCGGCCAGGCTGCAAAAGAAGGAGGTGTTGGTTGGGTGC TGCGTCTTAATCACCTGCCGACAGCATAACTGGCCCCTAACAATGGGGGCCATCTGCACG CTGTTGTATGCAGATTTGGATGTGTTTTCTAGCACTTACATGCAGATAGTGAAGCTCCTG GGACTGGATGTGCCATCTCTGTGCTTGGCAGAACTGGTGAAGACCTATTGCAGCAGCTTC AAACTGTTCCAAGCTTCACCTTCTGTGCCAGCCAAATACGTGGAAGACAAAGAGAAGATG CTGTCTCGAACAATGCAGTTGGTGGAGCTGGCAAATGAGACGTGGCTGGTGACCGGGAGG CATCCCTTGCCCGTCATCACTGCTGCGACTTTCCTGGCTTGGCAGTCGCTGCAGCCTGCA GATCGGCTTTCATGTTCCCTTGCCCGATTTTGTAAATTGGCAAATGTGGACCTGCCCTAC CCGGCGTCCTCCCGCCTGCAGGAGCTGCTGGCTGTGCTGCTGCGGATGGCTGAGCAGCTG GCCTGGTTACGAGTTCTGAGACTTGACAAACGGTCTGTGGTGAAGCACATCGGTGACCTT CTCCAGCACCGCCAGTCACTGGTCCGCTCTGCCTTTCGGGATGGGACAGCAGAAGTGGAG ACCCGAGAGAAGGAGCCACCGGGGTGGGGACAGGGGCAAGGAGAAGGGGAGGTGGGAAAT AATTCCTTAGGTTTACCCCAGGGGAAGCGGCCGGCCAGTCCTGCCCTTCTCTTGCCACCC TGCATGTTGAAGTCCCCGAAGCGGATCTGCCCTGTACCCCCTGTCTCCACTGTCACTGGA GATGAGAACATTTCTGATAGTGAAATAGAACAGTATTTGCGTACCCCTCAGGAAGTTAGG GACTTTCAGAGAGCCCAGGCTGCTAGACAGGCTGCCACGAGTGTCCCTAACCCTCCCTGA Clone variation with respect to NM_018310.3 >OriGene 5' read for NM_018310 unedited
GCATTTGTATACGACTCCTATAGGCGGCNCGCGAATTCGCACGAGGCTGAAGGCTTCCGC GGCTTTGGGGCCGGTTGTGAGATGCCAGGCAGAGGCCGCTGCCCGGACTGCGGCTCCACG GAGCTGGTGGAAGACTCGCACTATTCGCAGAGCCAGCTGGTGTGCTCCGACTGCGGCTGC GTGGTCACCGAGGGGGTCCTTACCACTACCTTCAGCGACGAGGGCAATCTCCGAGAGGTA ACATATTCCCGAAGCACAGGGGAAAACGAACAAGTTAGTCGCAGCCAGCAACGAGGTCTC CGGCGAGTGAGAGACCTTTGTCGAGTTCTGCAGTTGCCACCAACATTTGAGGATACCGCG GTTGCCTACTACCAACAGGCATATCGGCACTCTGGCATCCGAGCGGCCAGGCTGCAAAAG AAGGAGGTGTTGGTTGGGTGCTGCGTCTTAATCACCTGCCGACAGCATAACTGGCCCCTA ACAATGGGGGCCATCTGCACGCTGTTGTATGCAGATTTGGATGTGTTTTCTAGCACTTAC ATGCAGATAGTGAAGCTCCTGGGACTGGATGTGCCATCTCTGTGCTTGGCAGAACTGGTG AAGACCTATTGCAGCAGCTTCAAACTGTTCCAAGCTTCACCTTCTGTGCCAGCCAAATAC GTGGAAGACAAAGAGAAGATGCTGTCTCGAACAATGCAGTTGGTGGAGCTGGCAAATGAG ACGTGGCTGGTGACCGGGAGGCATCCCTTGCCCGTCATCACTGCTGCGACTNTCCTGGCT TGGCAGTCGCTGCAGCCTGCAGATCGGCTNTCATGTTCCCTTGCCCGATTTTGTAATTGG GCAATGTGGACCTGCCCTACCCNGCGTNCTCCGNCTGCNAGACTGCTGGCTGTGCTTGCT GCGATGCTGACACTGCCTGNTACAGTCTAACTGACACGTCTGGGGAGCCACGTGACTTTC CACGCGTCTGTCGTTGCTTCGATGACACAATGAAC >OriGene 3' read for NM_018310 unedited
CGGCCGCAATCTANAATCGAGTTTTTTTTTTTTTTTTTTAAAAACTCGGGTTTTATACAA TAGAATGTTTTCTAGCAGATGCCTCTTGTTTTAATATATTAAAATTTTGCAAAGCCCTTT GAGCTACTGCCTTAGTCTACCCACTGTCCTTTTGTTATGAGGTAGAGGATCTCATGACAC CATACACACAAACCCATCATTGCCTGTGAATGCACGTAGGGCCAGAATTCCCCAGTTCCC GCTCCTCTGAGGGTTGATACTGCTGGGAATGCCAACCACTCCACAAGCAGAGGGAAGCCC CCTCAGGCCTGCAGGAGGAGCCGCAGCAGTGTGTCCAATTCAAACCAGCAGCAAAGAGCC TGACATTTTCCCATCCATCTATGAGGAAAGCCATCTCACAGAACATGGACATAGGCAACT TGCTCTCCCACACCAAGGGATGGGAATCTCTCCTACCTATAGTCATCCCTGCACTCCTGA CTTTACTCCAGGACCCAGGGTCCAACTAATGGCAGAGCCCCTCTTGGTTCCTTCAAACAA GAAAAGCAATACCTACGGACTGGTGTACACTTCCATCCTTGGTTATAACAGGAATGTTAT CAAGCTGTCAGAACAGGATGAAGTGCTCCCAGTGGATATCCATCAGGGAGGGTTAGGGAC ACTCGTGGCAGCCTGTCTAGCAGCCTGGGCTCTCTGAAAGTCCCTAACTTCCTGAGGGGT ACGCAAATACTGTTCTATTTCACTATCAGAAATGTTCTCATCTCCAGTGACAGTGGAGAC AGGGGGTACAGGGCAGATCCGCTTCGGGGACTTCACATGCANGGTGGGCAAGAAAANGGC AGACTGNNNCGNCGCTTCCCCTGGGTAAACCTAGGGAAATATTCCCACTNNCCTTCTCTT GCCCCTGTCCCNACCCGGNNGCTCTTTCTCGGTCTCCACTCTGCTGCCCATCCGAAGGCA AGCGGACC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018310 |
| Insert Size | 1910 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018310.2, NP_060780.2 |
| RefSeq Size | 1978 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 55290 |
| UniProt ID | Q9HAW0 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202314 | BRF2 (Myc-DDK-tagged)-Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2) |
CNY 3656.00 |
|
| RC202314L3 | Lenti ORF clone of Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC202314L4 | Lenti ORF clone of Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2), mGFP tagged |
CNY 5890.00 |
|
| RG202314 | BRF2 (tGFP-tagged) - Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2) |
CNY 5256.00 |
