PPP2R2D (NM_001003656) Human Untagged Clone
CAT＃: SC113475
PPP2R2D (untagged)-Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2
CNY 2400.00
CNY 2950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MDS026 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113475 sequence for NM_001003656 edited (data generated by NextGen Sequencing)
ATGGAGGAGCTGACCGAAGTCATCACTGCAGCCGAGTTCCACCCGCACCAGTGCAACGTG TTCGTCTACAGCAGTAGCAAAGGGACCATCCGCCTGTGTGACATGCGCTCCTCGGCCCTG TGCGACAGACACTCCAAGTTTTTTGAAGAGCCTGAAGATCCCAGCAGTAGGTCCTTCTTC TCAGAAATAATTTCATCCATATCCGATGTAAAATTCAGTCATAGTGGGCGGTACATGATG ACCAGAGACTACCTGTCGGTGAAGGTGTGGGACCTCAACATGGAGAGCAGGCCGGTGGAG ACCCACCAGGTCCACGAGTACCTGCGCAGCAAGCTCTGCTCTCTCTATGAGAACGACTGC ATCTTTGACAAGTTTGAGTGTTGCTGGAACGGTTCGGATAGCGCCATCATGACCGGGTCC TATAACAACTTCTTCAGGATGTTTGATAGAGACACGCGGAGGGATGTGACCCTGGAGGCC TCGAGAGAGAGCAGCAAACCGCGCGCCAGCCTCAAACCCCGGAAGGTGTGTACGGGGGGT AAGCGGAGGAAAGACGAGATCAGTGTGGACAGTCTGGACTTCAACAAGAAGATCCTGCAC ACAGCCTGGCACCCCGTGGACAATGTCATTGCCGTGGCTGCCACCAATAACTTGTACATA TTCCAGGACAAAATCAACTAG Clone variation with respect to NM_001003656.1 >OriGene 5' read for NM_001003656 unedited
ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGAAAAGGGAAAAAAATCCC TCCCCGGCGGCGGCGGCGGCGGCGGCGGCGCCGGCGGTGGTGGCGGCCCCGGGGCTGAGC GCTCGGCTGCAGCGGCGCGGAGGCCGTCTCCCTGGTCTGCCGCGGTCCCCGCCCGTCCCG CCGCCGGCTGCCATGGCAGGAGCCGGAGGCGGCGGCTGCCCCGCGGGCGGCAACGACTTC CAGTGGTGCTTCTCGCAGGTCAAGGGGGCCATCGACGAGGACGTGGCCGAAGAATAAAAG CCGCCCTCATTCTAGGGGAGAATATAATGTTTACAGCACCTTTCAAAGTCATGAACCGGA GTTTGACTATTTGAAAAGTCTAGAAATTGAGGAAAAAATTAATAAAATTAGGTGGTTACC ACAACAGAATGCTGCTCATTTTCTACTGTCTACAAATGATAAAACTATAAAATTATGGAA AATAAGTGAACGGGATAAAAGAGCAGAAGGTTATAACCTGAAAGACGAAGATGGAAGACT TCGAGACCCATTTAGGATCACGGCGCTACGGATGACCTGAGAATTAATTTATGGCACTTA AAAATACAGATAGAAGCTTTAACATCGTGGACATCAAGCCTGCTAACATGGAGGAGCTGA CCGAAGTCATCACTGCAGCCGAGTTCCACCCGCACCAGTGCAACGTGTTCGTCTACAGCA GTAGCAAAGGGACCATCCGCCTGTGTGACATGCGCTCCTCGGCGCTGTGCGACAGACACT CCAAGTTTTTTGAAGAGGCTGAGAATCCAGCAGTAGGTCCCTTCTTTCAAAAATAATCTA TTCATATTCGATGTAAAATTAGTCCTATGGGCCGTTCATGATGACCACAAACTACCTGTG GTGAAGGTGTGGGACCTCACAGGAAAGCAGGCCGGGGGACCCCCAGGCCA >OriGene 3' read for NM_001003656 unedited
GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGATTTAAAAACACT TTATAGTGTGTTGTTTTTATTGAGCGCTCACACCCGAAGGGGTGGCGGCGGATGCTGTGG GTGAGTGGGGCCGCCTGAGCCTGCTCGGGCCACATCCACACATCCACAGAGTCCACCTGG ACTCGGAGGAAGGCCGAGAGGACACGGACGGTGGCCACACCACGCTGCAGTGAAGGCCCA AGTGTGATGGCAGAGAAAGAGGGAAAGTTGGAGAAAGAGCGGTATCTGACAATAACTTTT CTCTTGGATGTTAATTTTTTGGTCTATAAATTGGAAAGGAAGGCTCGGACTGAAATAAAT ACATTTATTCTGAGTAATGACCTTTTGGGAGCAGTGTCCGTCAACTCTGCTTCGAGAGCG TCTCCACGTGAGCAGCAGCGCCTGTCTCGCGCCTCGCCGAGGCGGAACCACACCGGGCCT CCAGCGGAGGCGGCTTTCTCCTGTGTCTGTGAAGGGAAGTAGCAGGTGCGTCACTGTTCT TAATGGAGCGGACAATGATGCCTTTTCTCTGACAGAAAAATGTCCGGCTTAACTATGCAA GACAAGACTTGGTCCTCACGTTCGCGTCTCTAGTTGATTNTGTCCTGGAATATGTACAAG TTATTGGTGGCAGCCACGGCAATGACATTGTCCACGGGGTGCCANGCTGTGTGCAGGATC TTCTTGTTGAAGTCCAGACTGTNCACACTGATCTCGTCTTTCCTCCGCTACCCCCCGTAC ACACCTCCGGGGNTTGAGGCTGGCGCGCGGTTTGCTGCTCTCTCTTCGAGCCTCCANGGT CACATCCCTCCGGGTGTNCTCATCAAACATNCTGNANAAATNTGTATAGGACCCCGTCAT GATGGCGCTATTCGAACCGTTCAGCACACTCAACTGTCAAGATGCAGTCGTCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001003656 |
| Insert Size | 1960 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001003656.1, NP_001003656.1 |
| RefSeq Size | 2091 bp |
| RefSeq ORF | 681 bp |
| Locus ID | 55844 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Tight junction |
| Gene Summary | B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an additional exon in the 5' region and lacks an internal segment in the middle region, as compared to variant 1. It uses a downstream AUG start codon and encodes isoform 2 which has a shorter N-terminus, as compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221048 | PPP2R2D (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2 |
CNY 2400.00 |
|
| RC221048L1 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, Myc-DDK-tagged |
CNY 4800.00 |
|
| RC221048L2 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RC221048L3 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC221048L4 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG221048 | PPP2R2D (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2 |
CNY 4000.00 |
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