TMEM165 (NM_018475) Human Untagged Clone
CAT＃: SC113448
TMEM165 (untagged)-Human transmembrane protein 165 (TMEM165)
CNY 2400.00
CNY 6270.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDG2K; FT27; GDT1; SLC64A1; TMPT27; TPARL |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113448 sequence for NM_018475 edited (data generated by NextGen Sequencing)
ATGGCGGCCGCGGCTCCGGGGAACGGCCGCGCATCGGCGCCCCGGCTGCTTCTGCTCTTT CTGGTTCCGCTGCTGTGGGCCCCGGCTGCGGTCCGGGCCGGCCCAGATGAAGACCTTAGC CACCGGAACAAAGAACCGCCGGCGCCGGCCCAGCAGCTGCAGCCGCAGCCTGTGGCTGTG CAGGGCCCCGAGCCGGCCCGGGTCGAGAAAATATTTACACCAGCAGCTCCAGTTCATACC AATAAAGAAGATCCTGCTACCCAAACTAATTTGGGATTTATCCATGCATTTGTCGCTGCC ACATCAGTTATTATTGTATCTGAATTGGGTGATAAGACATTTTTTATAGCAGCCATCATG GCAATGCGCTATAACCGCCTGACCGTGCTGGCTGGTGCAATGCTTGCCTTGGGACTAATG ACATGCTTGTCAGTTTTGTTTGGCTATGCCACCACAGTCATCCCCAGGGTCTATACATAC TATGTTTCAACTGTATTATTTGCCATTTTTGGCATTAGAATGCTTCGGGAAGGCTTAAAG ATGAGCCCTGATGAGGGTCAAGAGGAACTGGAAGAAGTTCAAGCTGAATTAAAGAAGAAA GATGAAGAATTTCAACGAACCAAACTTTTAAATGGACCGGGAGATGTTGAAACGGGTACA AGCATAACAGTACCTCAGAAAAAGTGGTTGCATTTTATTTCACCCATTTTTGTTCAAGCT CTTACATTAACATTCTTAGCAGAATGGGGTGATCGCTCTCAACTAACTACAATTGTATTG GCAGCTAGAGAGGACCCCTATGGTGTAGCCGTGGGTGGAACTGTGGGGCACTGCCTGTGC ACGGGATTGGCAGTAATTGGAGGAAGAATGATAGCACAGAAAATCTCTGTCAGAACTGTG ACAATCATAGGAGGCATCGTTTTTTTGGCGTTTGCATTTTCTGCACTATTTATAAGCCCT GATTCTGGTTTTTAA Clone variation with respect to NM_018475.3 18 a=>g;302 t=>c >OriGene 5' read for NM_018475 unedited
GGGTTCAGATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCAGAGGCCTCGTGCC GAATTCGGCACGAGGGGAGAGGACGGGCGCCGAGCCGGGGCTGCGGACTTCGGCCTGCCC CTCACCTCACTCCCGCTGCTTGCACCTCCCGGATGGTGCTGACTGCTCCCTAAGCGGCGG CGGCGGCGAGTCGTGAGGACGCGCCGCGGAGGCTGTTCGGGGTCGAGGCTTCCCGTCGCC GGCACTTCCTCTTGCGGCGCCCGTGCGCGGCCGGCCCGGCAGGCGGGATGGCGGCCGCGG CTCCGGGGAACGGCCGCGCATCGGCGCCCCGGCTGCTTCTGCTCTTTCTGGTTCCGCTGC TGTGGGCCCCGGCTGCGGTCCGGGCCGGCCCAGATGAAGACCTTAGCCACCGGAACAAAG AACCGCCGGCGCCGGCCCAGCAGCTGCAGCCGCAGCCTGTGGCTGTGCAGGGCCCCGAGC CGGCCCGGGTCGAGAAAATATTTACACCAGCAGCTCCAGTTCATACCAATAAAGAAGATC CTGCTACCCAAACTAATTTGGGATTTATCCATGCATTTGTCGCTGCCACATCAGTTATTA TTGTATCTGAATTGGGTGATAAGACATTTTTTATAGCAGCCATCCATGGCAATGCGCTAT AACCGCCTGACCGTGCTGGCTGGTGCAATGCTTGCCTTGGGACTAATGACATGCTTGTCA GTTTTGTTTGGCTATGCCACCACAGTCATCCCCCAGGGTCTATACATACTATGTTTCAAC TGTATTATTTGCCATTTTTTGGCATTANAATTGCTCCGGGAAAGCCTTACAGATGACCCC TGATGAGGGTCACGAGGACCTGGCAGAAAGTCAGCCTGATTAAAGAAGAAAGATGACAT >OriGene 3' read for NM_018475 unedited
TAGAGTCGAGTTTTTTTTTTTTTTTTTTACTGGTCATACCACTTTTATTTATATTGTTAT CTATGTTACATTGTTTATTCAATATAGAAAAAATATGAATACATTCATAGTATTCTCTTT TAATTTGGTAATTCCACATTGTCAAATATTGACTGTTTTTATATTGGGTGTCCTACCCAA TTAAAAAAAAAAGAGGAAGAATGACCTGGCAGACAGTTCTTGTTGTGCCATGTTATTGAT ATAACAGCAATATCATATATGTCTCTTTTTTTAAACACCTAGTTTTGAAAAGACCAATGA TTAAACTACTGTTTACTGGCTCAGGGAAATGTTTTAAGAAATAGAAATTAATTCATTCTT TCCCCAAGAAAAACTTTAAGGTAAACATCTTAAAACACCTAAGTGATGAAGGAGAAAACT TGTATTCCTTAAAAAGGGGTCAGTGCTGAAAGAAAATTGCATGGTAGACCCCACTGCACA ACGGTTCTGCACCAAAAGAAAAATGAGGACCATATTAACATAATTATGTTGGAGAGAAGA AAACCCACACTTAGAAGTCAGGTTTCTTTTTAAACTNCTTGTTACAATAGAATCATGATT ATATCTCAGACATAATAATGGGTCAAACTCACAAAATCAGTGCTACAAAATACAGNATTT TTCCATCACTTTTAGTTGCACTGTACACTATGTACAGAAAGATAATACTACCTTATTTAA CCTAATATAGATGAACAACCGCGTTAAAACCAGAATTACGGCTATAAATAGTGCAGAAAA TGCAACGCCAAAAAACGATGCCTCCTTGATTGTCACAGTTCTGACGAAATTTTTGGGGCT TCATTCTTTCTCCAATACTGCCATTCCGTGCCAAGCAATGCCCAAAGTTCACCACGGTCC CATAAGGTTCCTTTTANCGCCAACCACTGTAGTATTTGAACGCACACCCATCTGTTANAA TGTATGTAGACGTGAACAAATGGGAAAAATGCCACCTTTTGGAGCCCGTTAGCTTACCCT TCACCTTCCGCCCTAAAGATGGTCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018475 |
| Insert Size | 1830 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018475.2, NP_060945.2 |
| RefSeq Size | 1715 bp |
| RefSeq ORF | 975 bp |
| Locus ID | 55858 |
| UniProt ID | Q9HC07 |
| Domains | UPF0016 |
| Protein Families | Transmembrane |
| Gene Summary | This gene encodes a predicted transmembrane protein with a perinuclear Golgi-like distribution in fibroblasts. Mutations in this gene are associated with the autosomal recessive disorder congenital disorder of glycosylation, type IIk. Knockdown of this gene's expression causes decreased sialylation in HEK cells and suggests this gene plays a role in terminal Golgi glycosylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (1) represents the shorter transcript. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210474 | TMEM165 (Myc-DDK-tagged)-Human transmembrane protein 165 (TMEM165) |
CNY 2400.00 |
|
| RC210474L3 | Lenti ORF clone of Human transmembrane protein 165 (TMEM165), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC210474L4 | Lenti ORF clone of Human transmembrane protein 165 (TMEM165), mGFP tagged |
CNY 5890.00 |
|
| RG210474 | TMEM165 (tGFP-tagged) - Human transmembrane protein 165 (TMEM165) |
CNY 4000.00 |
|
| SC321033 | TMEM165 (untagged)-Human transmembrane protein 165 (TMEM165) |
CNY 2400.00 |
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