C3IP1 (KLHL12) (NM_021633) Human Untagged Clone
CAT＃: SC112907
KLHL12 (untagged)-Human kelch-like 12 (Drosophila) (KLHL12)
CNY 6352.00
| Cited in 1 publication. |
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C3IP1; DKIR |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112907 sequence for NM_021633 edited (data generated by NextGen Sequencing)
ATGGGAGGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTC AATTCAATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAG CAGAAAGACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCC ATGTTCACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACT GCCTCTACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACGGTACATGTGACAGTG GAGAATGTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAA GCCTGCTGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGAT TTTGCTGAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAG CATTTTCCTGAAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAA AAGCTAATCAAGTGCGACGAAATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTC ATCAACTGGGTGAAGCATGCCAAGAAAGAGCGGGAAGAATCCTTGCCTAACCTGCTACAG TATGTGCGGATGCCCCTACTAACCCCCAGGTATATCACAGATGTAATAGATGCTGAGCCT TTCATCCGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAAGTTTCATCTG AGGCCTGAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAAT GAAGTGCTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAG AAATATGACCCCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGT TATGTGGCCTCAGTGTCCCTTCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGT TCCCGCCTTAGTTCAGTGGAATGTCTAGACTACACAGCAGATGAGGATGGGGTCTGGTAT TCTGTGGCCCCTATGAATGTCCGACGAGGTCTTGCTGGAGCCACCACCCTGGGAGATATG ATCTATGTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGAT CCAAACATTGACCAGTGGAGCATGCTGGGAGATATGCAGACAGCCCGGGAAGGTGCCGGA CTCGTAGTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTA AATTCAGTTGAGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCC ACCAAGCGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGA TTTGATGGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGATTCCTGG ACAACTGTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGG AGACTCTATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTAT GACCCTATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCT GGTGTTTGTGTTCTCCGCGAGAAGTGA Clone variation with respect to NM_021633.2 1230 g=>a >OriGene 5' read for NM_021633 unedited
AGGGCGGGCCGCGAATTCGCACGAGGGTCGCGCGGCCGCAGGGCCAGACCCGGACGGCTC CAGAGCCTCCAGAGCCTCCGGGTCTGGGCGGCGCTTCGGCTCCTCCCGAGCCGCCTGCTA GCCCCGCGCCGCACTCCATCCCCACAGGCTGGGGACGGGCCCGGTGCGGCTGTGTGGGTT CGGGAGCGGAGTTGCAGAATCCAAGGACCCATTTTGTTCTTTCTCCGCACTGCTTTATGG GAGGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTCAATT CAATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAGCAGA AAGACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCCATGT TCACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACTGCCT CTACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACGGTACATGTGACAGTGGAGA ATGTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAAGCCT GCTGTGAGTTCTTAAAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTANGGGATTTT GCTGAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAGCAT TTCCTGGAAGTGTACAGATGGGAGAGTTCATTCTTCTGATCAGGGAGAGGGTGGAANAGC TATCAGTGCGACGAATTAGGTGGATTCTGAGAGCCAGCTTGGGAGCTGTCTCAACTGGGT GAGCATGCCAGAAGAGCGGGAAGAATCTTTGCTACTGCTCGTATGGGGCGGATGCCCCTA CTAACCCAGTTTNAAGATGNAATGATGA >OriGene 3' read for NM_021633 unedited
GGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGACCGTTGATTTT TAATATTTTCTTAAAAAAATACAAAGGAAATTAACTCTGTAGGTCAATACAACTCAGGGA AAGAGGGAAAAATGGAATTTCAGAGCAAAGGTTGTTTAGGTTATCACATTCCCACACTCC TAATACCCACAAAACAAGAATTTCACTCCATGACACAGAGGAACATTGAATGGTAGCTCA GAAATGTTGATAGCTGAGGTACTGAAACTAACAAAAGGATTTTGGTTGTCCTTGATTATT CTGTCCTGTGATGAATAAAATCTACACTAAAGGACAGGTAAGGAAAACTTATAGCAGAAA AAAGACTAGATGTACCAAACACAGCAGTACAAACCACTCCTTGGCAGACATGTGCTTCTA AAAGAATGGGGCAGTAATCAGGTAGCTGAACTACTAGGCTACTGTCACTCCCAGCCCATC CCCAAATAAATAGTGTGGAAGTGTAATAGTGTAGTAGTATTTGATCCAACAAAGAAAGGC TTTCACCCCCATTCAAGGGCAAACATTGCCATGGCTAGATGAGCCCTGGCAGGTAATAAG ATGACTGTAAAATCACAGGTTCTTCTAGTTTATCCCCAGTTATACTGAACTCCCACATTG ATGTCATCTTCTCTCATCCCAACATTTGGTCACTCTTTTATGCTGTCTNCCGTGCGAAAG ACCTCGGGGGAAATAAAAAAACCTTCAAATAATATACCCTTCTCAGGCTTCCAGTTATGT AAACTTCAGTGCTGGTCTAGGTCACTCCCAATCAATTCAGTTAATTCACTTAATTCTACT TCAATCTGAAAACAAATCCGACCTCCAAAGAAGGGTCAGAGAGGCTTTTTTCCCTGGCCC CAATTTTGGGGGGCTCTCCTTTTGGTTCCCCTAAGGGAACTGTTGCCCTCCTCCTGTTAT AAAAAATGTTGTTCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021633 |
| Insert Size | 3380 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021633.2, NP_067646.1 |
| RefSeq Size | 3338 bp |
| RefSeq ORF | 1707 bp |
| Locus ID | 59349 |
| UniProt ID | Q53G59 |
| Domains | BTB, Kelch |
| Gene Summary | This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (2) uses an alternate 5' terminal exon, resulting in a distinct 5' UTR and an in-frame downstream translational start site, compared to variant 1. This results in isoform 2, which has a shorter N-terminus compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Rotavirus NSP1 associates with components of the cullin RING ligase family of E3 ubiquitin ligases
,Lutz, LM;Pace, CR;Arnold, MM;,
J. Virol.
,PubMed ID 27099313
[KLHL12]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200894 | KLHL12 (Myc-DDK-tagged)-Human kelch-like 12 (Drosophila) (KLHL12) |
CNY 6344.00 |
|
| RC200894L1 | Lenti ORF clone of Human kelch-like 12 (Drosophila) (KLHL12), Myc-DDK-tagged |
CNY 8744.00 |
|
| RC200894L2 | Lenti ORF clone of Human kelch-like 12 (Drosophila) (KLHL12), mGFP tagged |
CNY 5890.00 |
|
| RC200894L3 | Lenti ORF clone of Human kelch-like 12 (Drosophila) (KLHL12), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC200894L4 | Lenti ORF clone of Human kelch-like 12 (Drosophila) (KLHL12), mGFP tagged |
CNY 8744.00 |
|
| RG200894 | KLHL12 (tGFP-tagged) - Human kelch-like 12 (Drosophila) (KLHL12) |
CNY 7944.00 |
