MPPE1 (NM_023075) Human Untagged Clone
CAT＃: SC112324
MPPE1 (untagged)-Human metallophosphoesterase 1 (MPPE1)
CNY 3656.00
CNY 6460.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Cdc1; PGAP5 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112324 sequence for NM_023075 edited (data generated by NextGen Sequencing)
ATGGCGATGATCGAATTGGGGTTTGGAAGACAGAATTTTCATCCATTAAAGAGGAAGAGT TCATTGCTGTTGAAACTCATAGCTGTTGTCTTTGCTGTGCTTCTATTTTGTGAATTTTTA ATCTATTACTTAGCGATCTTTCAGTGTAATTGGCCTGAAGTGAAAACCACAGCCTCTGAT GGTGAACAGACCACACGTGAGCCTGTGCTCAAAGCCATGTTTTTGGCTGACACCCATTTG CTTGGGGAATTCCTAGGCCACTGGCTGGACAAATTACGAAGGGAATGGCAGATGGAGAGA GCGTTCCAGACAGCTCTGTGGTTGCTGCAGCCGGAAGTCGTCTTCATCCTGGGGGATATC TTTGATGAAGGGAAGTGGAGCACCCCTGAGGCCTGGGCGGATGATGTGGAGCGGTTTCAG AAAATGTTCAGACACCCAAGTCATGTACAGCTGAAGGTAGTTGCTGGAAACCATGACATT GGCTTCCATTATGAGATGAACACATACAAAGTAGAACGCTTTGAGAAAGTGTTCAGCTCT GAAAGACTGTTTTCTTGGAAAGGCATTAACTTTGTGATGGTCAACAGCGTGGCGCTGAAC GGGGATGGCTGTGGCATCTGCTCTGAAACAGAAGCAGAGCTCATTGAAGTTTCTCACAGA CTGAACTGCTCCCGAGAGGCACGTGGCTCCAGCCGGTGTGGACCTGGGCCTCTGCTGCCC ACGTCTGCCCCTGTCCTCCTGCAGCATTATCCTCTGTATCGGAGAAGTGATGCTAACTGT TCTGGGGAAGACGCTGCTCCTGTAGAGGAAAGGGACATCCCATTTAAGGAGAACTATGAC GTGCTTTCACGGGAGGCATCACAAAAGCTGCTGTGGTGGCTCCAGCCGCGCCTGGTTCTC AGTGGCCACACGCACAGCGCCTGCGAGGTGCACCACGGGGGCCGAGTCCCCGAGCTCAGC GTCCCATCTTTCAGTTGGAGGAACAGAAACAACCCCAGTTTCATCATGGGTAGCATCACG CCCACAGACTACACCCTCTCCAAGTGCTACCTCCCACGTGAGGATGTGGTTTTGATCATC TACTGTGGAGTGGTGGGCTTCCTTGTGGTCCTCACACTCACTCACTTTGGGCTTCTAGCC TCACCTTTTCTTTCTGGTTTGAACTTGCTCGGAAAGCGTAAGACAAGATGA Clone variation with respect to NM_023075.5 803 c=>t >OriGene 5' read for NM_023075 unedited
CTGTCACCATTTGAACTCCATCATATAGGCGGCCGCGCAATCGGCACGAGGGTAAAAGTA TAAGACGATTAATTTGGCCAATGTAGATAGGATTATGTAACTCATTAGTTCTTAATAGAG GGAGGTCGTGATACCTGTATTATTAGGGAACATAACTGAATTCTTTTCCTTTTCCCGTAG GTGCCCAGCTAAGACCTGAATGCCATCACCCTCCCCAGGGCTCTGCAGTTTTCTCGTGGT GAACCCTTGATGGATTTGTTGTTGCTTGAGAAATGGCGATGATCGAATTGGGGTTTGGAA GACAGAATTTTCATCCATTAAAGAGGAAGAGTTCATTGCTGTTGAAACTCATAGCTGTTG TCTTTGCTGTGCTTCTATTTTGTGAATTTTTAATCTATTACTTAGCGATCTTTCAGTGTA ATTGGCCTGAAGTGAAAACCACAGCCTCTGATGGTGAACAGACCACACGTGAGCCTGTGC TCAAAGCCATGTTTTTGGCTGACACCCATTTGCTTGGGGAATTCCTAGGCCACTGGCTGG ACAAATTACTAAGGGAATGGCAGATGGAGAGAGCGTTCCAGACAGCTCTGTGGTTGCTGC AGCCGGAAGTCGTCTTCATCCTGNGGGATATCTTTGATGAAGGGAAGTGGAGCACCCCTG AGGCCTGNGCGGATGATGTGGAGCGGTTTCAGAAAATGTTCAGACACCCAAGTCATGTAC AGCTGACGGTAGTTGCTGGAAACCATGACATTGGCTTCCATTATGAGATGAACACATACA AAGTAGAACGCTTTGAGACAGTGTTCAGCTCTGAAAGACTGTTTTCAGGCCAAGGCATTA ACTATGTGATGATCACCAGCGTTGCGCTTCACGGGGCATGGCTGTGGCATCTGCTCTGAA ACAGAGCAGAGCTCATTGAATTTCTCCAGACTGG >OriGene 3' read for NM_023075 unedited
ATCTATGNNACCGCGGCCGCAATTCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTCAACAGGAAAAGGGAAACAGCCCAATTTTTATTTATGTA TACACATAATCCCAAGTGGGGGCTGGGGCCACCAGCCCCTTCCTGGGGGAACAAGGACTG TCGGGCTTGTGAGTGACAACTTTAATAGCTTTTACATACTGGACAAATGCAACCTTTGAT GATACATATTTTTGATAAAAATGAAAAAACAAATTTGTTGAAAAGTCCCTGTCCCCTTTT ATAGCATGAAAACAGTCCAATCAACTATTTATTTTGCAGTTACTCTTTTCAGGGATTGAA AATTTTTGGGCTGTGCAAAAAAACGGCCTGTAATTGGTCTAATCATCCACTTGATTCTAA CATGATCTTTGCCAAAAGTTCCATTTCTTGGGCTTGGATATTAATAAGGGCGCCTGCTCT TAATTTTGTCTTACCCTTTCCGAGCAAGTTTAAACCAAAAAAAAAAGGGGAGGCTAAAAC CCCAAAGGGAGGGAGTGTGAGGACCACAAGGAGCCCCACCCTCTCACAGTAGTATGATCA AAACCCCTTCCTCCCCTGGAAGGTATACTTTGAGAAGGTGGTATCCTGTGGCCGCTATCC CCCCCATGATGAACCTGGGGTTGTTTTTGTTCCTCCATCTGCAAAATGGGCCCCTGATTC CGGGGCACTGCCCCCTTTGGTGCCTCCACAAGCCTTTGTCCGTGTGGCCATCATAAAAAA CGTCCGGGTTGGGAAACCTACACTATCCTTTCGGACGCCCTTCCGTGCAAACCCCTTTAA ATTTCCCTTAAAAGGGGCGGTCCTCTTTCTTCCAAGGCACACCGTTTTATCCCCAAACGT CTCCCTCCCCTTTCCGTTACCCAAGAAACCTTCTCTGAAGTCACAGGTCACAACTGGTTA CCACCGCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_023075 |
| Insert Size | 1890 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_023075.3, NP_075563.3 |
| RefSeq Size | 2825 bp |
| RefSeq ORF | 1191 bp |
| Locus ID | 65258 |
| UniProt ID | Q53F39 |
| Domains | Metallophos |
| Protein Families | Transmembrane |
| Gene Summary | Metallophosphoesterase required for transport of GPI-anchor proteins from the endoplasmic reticulum to the Golgi. Acts in lipid remodeling steps of GPI-anchor maturation by mediating the removal of a side-chain ethanolamine-phosphate (EtNP) from the second Man (Man2) of the GPI intermediate, an essential step for efficient transport of GPI-anchor proteins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218489 | MPPE1 (Myc-DDK-tagged)-Human metallophosphoesterase 1 (MPPE1) |
CNY 3656.00 |
|
| RC218489L3 | Lenti-ORF clone of MPPE1 (Myc-DDK-tagged)-Human metallophosphoesterase 1 (MPPE1) |
CNY 5890.00 |
|
| RC218489L4 | Lenti-ORF clone of MPPE1 (mGFP-tagged)-Human metallophosphoesterase 1 (MPPE1) |
CNY 5890.00 |
|
| RG218489 | MPPE1 (tGFP-tagged) - Human metallophosphoesterase 1 (MPPE1) |
CNY 4370.00 |
