CDK5RAP3 (NM_025197) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C53; C53, IC53, HSF-27, MST016, OK/SW-cl.114; CDK5 regulatory subunit associated protein 3; HSF-27; IC53; ischemic heart CDK5 activator-binding protein C53; LXXLL/leucine-zipper-containing ARFbinding protein; LZAP; MST016; OK/SW-cl.114
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_025197, the custom clone sequence may differ by one or more nucleotides ATGGAGGACCATCAGCACGTGCCCATCGACATCCAGACCAGCAAGCTGCTCGATTGGCTGGTGGACAGAA GGCACTGCAGCCTGAAATGGCAGAGTCTGGTGCTGACGATCCGCGAGAAGATCAATGCTGCCATCCAGGA CATGCCAGAGAGCGAAGAGATCGCCCAGCTGCTGTCTGGGTCCTACATTCACTACTTTCACTGCCTAAGA ATCCTGGACCTTCTCAAAGGCACAGAGGCCTCCACGAAGAATATTTTTGGCCGATACTCTTCACAGCGGA TGAAGGATTGGCAGGAGATTATAGCTCTGTATGAGAAGGACAACACCTACTTAGGTAAAGTGGCCCGGCC TGGGAGCCCTGGTATCCATGGGGAAGCCCACTCTCAGAGTTCTGAGATACCAGGCTTATAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_025197 unedited GGTGCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTCTGGTGC TGACGATCCGCGAGAAGACAATGCTGCCATCCAGGACATGCCAGAGAGCGAAGAGATCGC CCAGCTGCTGTCTGGGTCCTACATTCACTACTTTCACTGCCTAAGAATCCTGGACCTTCT CAAAGGCACAGAGGCCTCCACGAAGAATATTTTTGGCCGATACTCTTCACAGCGGATGAA GGATTGGCAGGAGATTATAGCTCTGTATGAGAAGGACAACACCTACTTAGGTAAAGTGGC CCGGCCTGGGAGCCCTGGTATCCATGGGGAAGCCCACTCTCAGAGTTCTGAGATACCAGG CTTATAGGAGGCACAGTCTGTGAGTGGGAAGAGACTGGAGTGTAGATGTTGCCCATTTGT AGGTGGTAAAATCAATTGTTTTTGATGGAATTGATTTTCCCTGAGTGGAGTGCTGGGGGA AGGAGGAGGTCCAGGCCGGTAGTGGCCATTCGCCGTGCCTCAGCGAGCAGGTGTGTGTGG GTCCTCCACCACTCACCTCTTGGTTACGGGAGTGTGGGCTGCCCCCACCCNCACCCCCGC ACCCCCATTCTACACAAGGCAGAAGAGGGCACGGGTTTTTCTGGGAGCGAATATCAAGTG CCTGAGAGCAACTACAGGACTAACTGTGTNTGGGTTGGGGTGTAGTATAAATAATAATAA TGGCTAATATTTCCTGAGCATCTACTAAATGCAAGGAATTGTGCTTGGTGTGTCATGGTG GATTCTCTCTTGCATCTTCATGATAATGTTANTGGCGCTGTTTCCGATGAAGGGTTGATT ANAAGGGGNTAAACAACTGTCTTTAGCTCCCAGCTGGAACAGTGGGGCCTGGGAGCTGAC TTCGTGCTCTCACCACN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_025197 unedited CTTTGACTAACTAGNAAGGANAGATCTGTGGTTTGTCGCCCTNATAGNAACCACAGGGCC AATCACCACAGCTTCTTGTAGAGAACATGGAGAGTGCCAAGATCACCATCAGGTGCCGCT TCCTTCCTGTGGCTTTCCATCTTCCAGTCAGCCTGGTCTTTTGCCTTGAAGGGCCCCAAA ACAACAGCCCTGGCTATCATCTTCATCCCAAAAGCGGAGAAGATGGGCAGGCAAGAACAC GGAGGGTGTCACAGAGAGGTTCCCATCAGGTTCACAGGGCGCCCGCTGTACCTCTTGGAG ATGTCAGCTTCAATCAGCTTCTGCAGCTCCTTGGTCTTCTCCAGTAGCAGGTCCAGCTTA GGCTCCAGAGCCGCCTGCTCCTCAAGTGCCTCCTGCTGCTTCTGCACCATCAGCTCTTTC TTCAAAGCCAGCAGCTGGGACTGCTTCAGCTTTTGCTGGAGGAATTCAGTCACTCGGTCC ACATACCTTGGTGAGGCCAGGATCATAAACAGGTGTTGCAGCTGAAGACTGGTAAGCTTG CCAATCAGATCCTCCAGCACTGACACCATGGTAACCATCTTCTCTTTGGTCTGGCCCTGC ANGATGGCTGGAGCCAGCTGGAACTGGCTCACAGACAGGACATCTGCCTNCTCACTCAAC TNCACTGCTCTCTGGGCTAAGAAGATCTCAAGCTCCATGAGCTCATCAAGGAACTGATTC CGGGTCTCAGTGTATTCAAGCAGTGTCAGGGCATCTGGGCCCCTGGNCACACCTTCTGGA GCCTGNGTTCCCTGCTNCAGCACTGTGATCTGCANAGCACAGCATCGTCTCCCAGTCTAT CCATCACTNCAGNATCCTTGATCTGATCCGGAAGATGCCCATCGATCCAGCACCTCGCAG AATGCAGATNAGTCCCTCAGACTGCCTTACCCAAGTGGCCATCATCCATCTCTGCACTGC TCAGAGCTCTNAGGGGGGTCGTCACCAAGGCTTGTTCTGGCTCA
Restriction Sites	NotI-NotI
ACCN	NM_025197
Insert Size	2770 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_025197.1, NP_079473.1
RefSeq Size	2594 bp
RefSeq ORF	1260 bp
Locus ID	80279
Gene Summary	This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013] Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 encode the predicted isoform a.

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