CDC25A (NM_201567) Human Untagged Clone
CAT#: SC111607
CDC25A (untagged)-Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 2
CNY 7,790.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CDC25A2 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_201567, the custom clone sequence may differ by one or more nucleotides
ATGGAACTGGGCCCGGAGCCCCCGCACCGCCGCCGCCTGCTCTTCGCCTGCAGCCCCCCTCCCGCGTCGC AGCCCGTCGTGAAGGCGCTATTTGGCGCTTCAGCCGCCGGGGGACTGTCGCCTGTCACCAACCTGACCGT CACTATGGACCAGCTGCAGGGTCTGGGCAGTGATTATGAGCAACCACTGGAGGTGAAGAACAACAGTAAT CTGCAGAGAATGGGCTCCTCCGAGTCAACAGATTCAGGTTTCTGTCTAGATTCTCCTGGGCCATTGGACA GTAAAGAAAACCTTGAAAATCCTATGAGAAGAATACATTCCCTACCTCAGAAGCTGTTGGGATGTAGTCC AGCTCTGAAGAGGAGCCATTCTGATTCTCTTGACCATGACATCTTTCAGCTCATCGACCCAGATGAGAAC AAGGAAAATCTTTCCTCAAATGAAAGAGATAGCAGTGAACCAGGGAATTTCATTCCTCTTTTTACACCCC AGTCACCTGTGACAGCCACTTTGTCTGATGAGGATGATGGCTTCGTGGACCTTCTCGATGGAGAGAATCT GAAGAATGAGGAGGAGACCCCCTCGTGCATGGCAAGCCTCTGGACAGCTCCTCTCGTCATGAGAACTACA AACCTTGACAACCGATGCAAGCTGTTTGACTCCCCTTCCCTGTGTAGCTCCAGCACTCGGTCAGTGTTGA AGAGACCAGAACGATCTCAAGAGGAGTCTCCACCTGGAAGTACAAAGAGGAGGAAGAGCATGTCTGGGGC CAGCCCCAAAGAGTCAACTAATCCAGAGAAGGCCCATGAGACTCTTCATCAGTCTTTATCCCTGGCATCT TCCCCCAAAGGAACCATTGAGAACATTTTGGACAATGACCCAAGGGACCTTATAGGAGACTTCTCCAAGG GTTATCTCTTTCATACAGTTGCTGGGAAACATCAGGATTTAAAATACATCTCTCCAGAAATTATGGCATC TGTTTTGAATGGCAAGTTTGCCAACCTCATTAAAGAGTTTGTTATCATCGACTGTCGATACCCATATGAA TACGAGGGAGGCCACATCAAGGGTGCAGTGAACTTGCACATGGAAGAAGAGGTTGAAGACTTCTTATTGA AGAAGCCCATTGTACCTACTGATGGCAAGCGTGTCATTGTTGTGTTTCACTGCGAGTTTTCTTCTGAGAG AGGTCCCCGCATGTGCCGGTATGTGAGAGAGAGAGATCGCCTGGGTAATGAATACCCCAAACTCCACTAC CCTGAGCTGTATGTCCTGAAGGGGGGATACAAGGAGTTCTTTATGAAATGCCAGTCTTACTGTGAGCCCC CTAGCTACCGGCCCATGCACCACGAGGACTTTAAAGAAGACCTGAAGAAGTTCCGCACCAAGAGCCGGAC CTGGGCAGGGGAGAAGAGCAAGAGGGAGATGTACAGTCGTCTGAAGAAGCTCTGA >OriGene 5' read for NM_201567 unedited
ACTTATAGGCGGCCCGCGAATTCGGCACGAAGGCTCTGCGAGGCCGGGCGCCGCCATGNA ACTGGGCCCGGAGCCCCCGCACCGCCGCCGCCTGCTCTTCGCCTGCAGCCCCCCTCCCGC GTCGCAGCCCGTCGTGAAGGCGCTATTTGGCGCTTCAGCCGCCGGGGGACTGTCGCCTGT CACCAACCTGACCGTCACTATGGACCAGCTGCAGGGTCTGGGCAGTGATTATGAGCAACC ACTGGAGGTGAAGAACAACAGTAATCTGCAGAGAATGGGCTCCTCCGAGTCAACAGATTC AGTTTCTGTCTAGATTCTCCTGGGCCATTGGACAGTAAAGAAAACCTTGAAAATCCTATG AGAAGAATACATTCCCTACCTTAAGCTGTTGGGGAATGTAGTCCAGCTCTGAAGAGGAGC CATTCTGATTCTCTTGACCATGACATCTTTCAGCTCATCGACCCAGATGAGAACAAGGAA AATCTTTCCTCAAATGAAAAGAATAGCAGTGACCAGGGAATTTCATTCCTCTTTTTACAC CCCAGTCACCTGTGACGNCACTTTGTCTGAGGAGGATGATGGGTTTCGTGGACCTTCTCG ATGGAGAGATCTGAGATGGAGGGGGAGACCCCTCGTGCTGGAAGCCTCTGACAGTCCTCT CGTATGAGACTACAACCTTGCACCGGCAAAGCGTGTTGATCCTCCCCTGTGACTCCACCC GGCCAGGGTGGGGAGAACCNAACGACTCAAGAGGGTCCCCGGGAATAAAGGAGGAAAAAT ATTGTGGGGGGCACCCCAGAGCACTATCCNAAAGGCCAGAACTTTCTTAANCTTATCTGC TCTCCCAAGCCCTTGGAAAATTGGCAGACAGCCTTTAGGATTCTCCAGGTATCTTTAAAG GGGGAAAAAGGA >OriGene 3' read for NM_201567 unedited
AGCTTTGGACCCGCGGCCGCAATCTAGAAGTCGGTTTTTTTTTTTTTTTTTTTTGGGTTC AAGATCTTTTATTTTCAGAGCTTCCAACAGTTGGTTAGTAATGCTAGCTAAGCTGGTATA ATCTGAAGGCCATCCCACCTTTCTCTTTAGGCAGTCAACCAACAGAATCTCTTTCTTACA AACAATATCATTAACCAGTTGGAATCTGTCTCAATGCGCGTGTAGGAAGAAGTCCTCTCC CCCACATTTTTCCCAACAAGATTGTTTTATAGCCAAATGTAACTCAAACCCGTAACACAG CAACTAGCCATCTCCAGTATCACTGATTCCCACCCCAAGAAGTTGAGGTAAGAGGGGAGA GACTGTCTCCTGTTTAAGAAAAACCCACCTACACCTCAGTGAAGCCGTGATGGTAAGGAG GCTGGCACCAGAGGGCAGGGATGAGTAGGCACTGAAACAGGGCTCTCTAGAATCAATGAA GGCTGCCCCAGCTCCTTGGATGAGGTGGNAGTCTGAAACTCCCAGCCCCCTGGGCTCGCT AGCGCGGACGCCCGCGCCCNNCCCACACCCATTGGGTTTCCCCGCCACCCCCCGACCCAG GGGNAAGCGCGCACCGTGACACCGCCGCGCCCCTACCTCTCGCAGAGCACGGCCGGCCAG AGTCGGGCCGAGGGGCGNNACCCCAGCTACGGTGCCGCCAATGCCCACGGTCTCCGACCC CCCCTCCCCCCCCGCCNCGTCTGCACCCCCGCGCCCCGCCGTCGATGTGCCCGTTGGCGG ACCGCCGCCGCCCGCTCGGNACACCGCCCACCCGCGCCGCGACGCGATTCCACGAGGGCG GCGAGGCCGCCCGTCGCCGCAATGCCCGCGGAGCCACGCGGCCGACGTACGCTCCGCTAT AGCGGGTTGACACAGTCACCGCGCGCCCAGCGTCTNCAGACGAGTCGAGAGGGGCGAGCC TCATGCCGACGCAGCNGACGCGCAGAGGCGCAGTGAGCCGACACACGCGGACACGC |
Restriction Sites | NotI-NotI |
ACCN | NM_201567 |
Insert Size | 3220 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_201567.1, NP_963861.1 |
RefSeq Size | 3597 bp |
RefSeq ORF | 1455 bp |
Locus ID | 993 |
UniProt ID | P30304 |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | Cell cycle, Progesterone-mediated oocyte maturation |
Gene Summary | CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214325 | CDC25A (Myc-DDK-tagged)-Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 2 |
CNY 4,280.00 |
|
RC214325L3 | Lenti-ORF clone of CDC25A (Myc-DDK-tagged)-Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 2 |
CNY 6,180.00 |
|
RC214325L4 | Lenti-ORF clone of CDC25A (mGFP-tagged)-Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 2 |
CNY 6,180.00 |
|
RG214325 | CDC25A (tGFP-tagged) - Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 2 |
CNY 4,750.00 |