MRPS35 (NM_021821) Human Untagged Clone
CAT＃: SC111387
MRPS35 (untagged)-Human mitochondrial ribosomal protein S35 (MRPS35), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 2400.00
CNY 6270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HDCMD11P; MDS023; MRP-S28; MRPS28 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_021821, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCCGCCGCGCTCCCAGCATGGCTGTCTCTGCAGTCGAGGGCAAGGACTCTGCGTGCATTCTCCA CTGCCGTCTACTCGGCCACTCCGGTCCCGACACCTAGCCTGCCGGAAAGAACACCCGGAAATGAAAGGCC ACCAAGAAGAAAGGCACTACCTCCTAGGACAGAGAAAATGGCTGTTGACCAGGACTGGCCTAGTGTTTAC CCAGTTGCAGCACCATTTAAACCCTCTGCAGTACCTCTTCCTGTTCGAATGGGTTATCCAGTAAAAAAGG GCGTGCCCATGGCAAAGGAGGGAAATCTAGAACTTTTAAAGATTCCCAATTTTCTGCATTTGACTCCTGT AGCAATTAAAAAGCACTGTGAAGCCCTTAAAGATTTTTGCACTGAGTGGCCAGCCGCACTGGACAGTGAC GAGAAATGTGAGAAGCATTTTCCAATTGAAATTGACAGCACTGATTATGTTTCATCAGGACCATCTGTTC GGAACCCCAGAGCACGAGTAGTAGTCTTAAGAGTAAAGCTTTCCAGTTTGAATTTAGATGATCACGCAAA GAAGAAATTAATTAAACTTGTAGGAGAGCGATACTGCAAGACCACAGATGTGCTTACCATCAAAACAGAT AGGTGCCCTTTAAGGAGGCAGAATTACGATTATGCAGTGTATCTACTAACAGTGTTATACCATGAGTCTT GGAATACTGAAGAATGGGAAAAAAGTAAGACTGAAGCAGACATGGAAGAGTATATATGGGAAAATAGCTC ATCAGAAAGAAATATCCTGGAAACGCTTCTCCAGATGAAAGCTGCTGAGAAAAATATGGAAATAAATAAA GAAGAGCTCCTTGGTACTAAAGAAATTGAAGAGTACAAAAAGTCTGTTGTTAGTCTTAAAAATGAGGAGG AAAATGAAAATTCCATTTCTCAGTACAAAGAATCCGTGAAGAGACTATTAAATGTGACATGA >OriGene 5' read for NM_021821 unedited
GTTCACTATTTGTAAACGACTCACTATAGGCGGCCGCGAATCGACACGAGGGGCCGCCGC GCTCCCAGCATGGCTGTCTCTGCAGTCGAGGGCAAGGACTCTGCGTGCATTCTCCACTGC CGTCTACTCGGCCACTCCGGTCCCGACACCTAGCCTGCCGGAAAGAACACCCGGAAATGA AAGGCCACCAAGAAGAAAGGCACTACCTCCTAGGACAGAGAAAATGGCTGTTGACCAGGA CTGGCCTAGTGTTTACCCAGTTGCAGCACCATTTAAACCCTCTGCAGTACCTCTTCCTGT TCGAATCGGTTATCCAGTAAAAAAGGGCGTGCCCATGGCAAAGGAGGGAAATCTAGAACT TTTAAAGATTCCCAATTTTCTGCATTTGACTCCTGTAGCAATTAAAAAGCACTGTGAAGC CCTTAAAGATTTTTGCACTGAGTGGCCAGCCGCACTGGACAGTGACGAGAAATGTGAGAA GCATTTTCCAATTGAAATTGACAGCACTGATTATGTTTCATCAGGACCATCTGTTCGGAA CCCCAGAGCACGAGTAGTAGTCTTAAGAGTGCCCTTTAAGGAGGCAGAATTACCATTATG CAGTGTATCTACTAACAGTGTTATACCATGAGTCTTGGAATACTGAAGAATGGGAAAAAA GTAAGACTGAAGCAGACCTGGAAGAGTATATATGGGAAAATAGCTCATCACAAAGAAATA TCCTGGGAACCGCTTCTCCAGATGAAAGCTGCTGAGAAAAATTTGGAAATAAATTAAGAA GAGCTTCCTTGGTACTAAAGAAAACGAAGAGTACAAAAGGCCCGTCGCTAGCCTTAAAAA TGAGGAGGAAATGACAATTCCTTTTCTCAGTCCAAAGAATCCGCGAAAAGACTATTAAAT GTGACTGGATTAGGGGGTTAAAAN >OriGene 3' read for NM_021821 unedited
ACCGCGGCCGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAACTCAGTAATTCCTT TATTTTGATTTTAAATTAATCACAACCATCGAAGTGGTAAGAAACAGCTGACAGGTGTAC TTAAAAATACTGAATTGACAGCTACATTGAATGCAGGGTTTCCCAGGGTAGTTCTTATTT TGTCACTTACTCCAATTACATTCAAGGTCGTTATGCCTCATCTTTTTCCTGAGCTGTGGC AGCTCTAACTGGGGCACCCAGAGAGATACATACCAGGTAATCTCCACTTCTACTTTTCTG GTAGCTTGGCCCTGGCAAAATGAGCCCCACAATCTAGAAAGTAGGATGCTAAACAAAGTT GAATCAACATATCTTTTTAGAAAATATCAGGTTAGAGAATACTCCTGAGGACCTGTTTCT AACCAGAGTTGACAAATGTGAAAAATGCATCAGCTAGACAGCAGTCATGTGAACACAGCC CGGAACTGCAAGTCAAGGAAATGGGTTCTGGGCCTGCCTTCCCAGGTAACCAAAACCATT TATTCAAAAGCAACTACTGAAATGCCAGCTTGTGGGGAAAGAAATGGGAAAGGGGATAAA AATCCCACATTAATGCATGTATTTACATGAAAACCAAAGCTATTTATGTAGAAATTAAAT CAAAACCCTTTAGACCAAGAAGAAATCAGAGACCTCCCAATCTATTGCCCCACTGTAAAA CATTAAAAACTCTGGAGATATCCTTGCCATTAAGCACTACCATGAAGGAAATAAATTTAA CCTTTGAGGAAAAAAACAGCATCTTAACATTTTCACATTTAATTCAATTTCAACCTGCTC CACTTTTCCTAAATTAATAATCCAGCCCATTTTCTCACCCCAAATTATGGCCATTACAAA GCCTTCCACGCATCTTTGAACG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021821 |
| Insert Size | 1790 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021821.1, NP_068593.1 |
| RefSeq Size | 1919 bp |
| RefSeq ORF | 684 bp |
| Locus ID | 60488 |
| UniProt ID | P82673 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq, Jul 2010] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205632 | MRPS35 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S35 (MRPS35), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 2400.00 |
|
| RC205632L3 | Lenti ORF clone of Human mitochondrial ribosomal protein S35 (MRPS35), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC205632L4 | Lenti ORF clone of Human mitochondrial ribosomal protein S35 (MRPS35), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG205632 | MRPS35 (tGFP-tagged) - Human mitochondrial ribosomal protein S35 (MRPS35), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4000.00 |
|
| SC317492 | MRPS35 (untagged)-Human mitochondrial ribosomal protein S35 (MRPS35), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 6270.00 |
|
| SC320954 | MRPS35 (untagged)-Human mitochondrial ribosomal protein S35 (MRPS35), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 2400.00 |
