ELL (NM_006532) Human Untagged Clone
CAT＃: SC111065
ELL (untagged)-Human elongation factor RNA polymerase II (ELL)
CNY 6944.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C19orf17; ELL1; MEN; PPP1R68 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111065 sequence for NM_006532 edited (data generated by NextGen Sequencing)
ATGGCGGCGCTGAAGGAGGATAGGAGCTACGGGCTGTCGTGCGGGCGGGTTAGCGACGGC AGCAAGGTGTCGGTGTTCCACGTGAAGCTCACCGACAGTGCCCTGAGGGCCTTCGAGAGC TACCGCGCCAGACAGGATTCTGTTTCACTGAGGCCATCTATCCGATTTCAAGGAAGCCAA GGGCACATCTCCATCCCCCAGCCTGACTGCCCCGCAGAGGCGCGGACGTTCTCCTTCTAC CTCTCCAACATCGGCCGCGACAACCCCCAGGGCAGCTTCGACTGCATCCAGCAGTATGTC TCCAGTCATGGGGAAGTTCACCTGGACTGCCTGGGCAGCATACAGGACAAGATCACGGTG TGTGCCACCGACGACTCCTACCAGAAGGCGCGGCAGAGCATGGCCCAGGCGGAGGAGGAG ACGCGGAGCCGAAGTGCCATTGTCATCAAGGCTGGAGGCCGCTACCTGGGCAAGAAGGTT CAGTTTCGGAAACCAGCCCCAGGTGCAACAGACGCGGTGCCCTCCCGGAAGCGGGCAACC CCCATCAACTTGGCGAGTGCCATCAGGAAGAGTGGTGCCAGTGCCGTGAGTGGGGGCAGC GGGGTGTCCCAGAGGCCCTTCCGTGACCGAGTGCTGCACCTCCTGGCACTACGGCCCTAC CGCAAGGCTGAGCTGCTGCTGCGACTGCAGAAGGACGGCCTGACGCAGGCGGACAAGGAC GCGCTGGATGGCCTCCTCCAGCAGGTGGCCAACATGAGTGCTAAGGACGGCACGTGTACA CTGCAGGACTGCATGTACAAGGATGTGCAGAAGGACTGGCCTGGCTACTCGGAGGGGGAC CAGCAGCTGCTGAAGCGGGTGCTCGTCCGGAAGCTGTGCCAGCCACAGAGCACTGGCAGC CTCCTTGGAGACCCTGCTGCCTCCAGCCCCCCAGGCGAGCGTGGGCGCTCGGCCTCGCCC CCACAGAAGCGGCTGCAGCCTCCTGATTTCATCGACCCCCTAGCCAACAAGAAACCCCGG ATATCGCACTTCACTCAGAGAGCTCAGCCTGCCGTCAACGGGAAGCTGGGCGTGCCCAAT GGCCGTGAGGCCTTGCTGCCCACCCCGGGCCCACCAGCCAGCACGGACACCCTCAGCTCC AGCACTCACCTGCCCCCGCGGCTGGAGCCCCCGAGGGCCCACGACCCCCTGGCCGATGTC AGCAATGACCTGGGCCACAGCAGCCGAGACTGTGAGCACGGAGAGGCGGCTGCCCCAGCC CCCACTGTGCGCCTCGGCCTGCCCCTGCTGACGGACTGTGCCCAGCCCAGCAGGCCACAC GGCAGCCCCTCGCGCAGCAAGCCCAAGAAGAAGTCCAAGAAGCACAAAGACAAGGAGAGG GCGGCTGAGGACAAGCCCCGGGCCCAGCTTCCAGACTGTGCACCTGCCACCCATGCCACC CCCGGAGCCCCAGCAGACACCCCAGGTTTAAACGGAACCTGCAGCGTTTCCAGTGTTCCC ACGTCCACGTCGGAGACGCCTGACTACTTGCTGAAGTACGCAGCCATCTCCTCTTCGGAG CAGCGCCAGAGCTACAAGAACGACTTCAATGCCGAGTACAGCGAGTACCGCGACCTGCAC GCCCGCATTGAGCGCATCACGCGGCGGTTCACCCAGCTCGACGCCCAGCTCCGGCAGCTC TCCCAGGGCTCCGAGGAGTATGAGACTACTCGAGGGCAGATTTTGCAGGAATATCGAAAA ATCAAAAAGACCAACACCAACTACAGCCAGGAGAAGCACCGCTGCGAGTACCTGCACAGC AAGCTGGCCCACATCAAGAGGCTCATCGCCGAGTACGACCAGCGGCAGCTGCAGGCTTGG CCCTAG Clone variation with respect to NM_006532.3 1222 g=>a >OriGene 5' read for NM_006532 unedited
CCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTC GGCACGAGGCGCAAGATGGCGGCGCTGAAGGAGGATAGGAGCTACGGGCTGTCGTGCGGG CGGGTTAGCGACGGCAGCAAGGTGTCGGTGTTCCACGTGAAGCTCACCGACAGTGCCCTG AGGGCCTTCGAGAGCTACCGCGCCAGACAGGATTCTGTTTCACTGAGGCCATCTATCCGA TTTCAAGGAAGCCAAGGGCACATCTCCATCCCCCAGCCTGACTGCCCCGCAGAGGCGCGG ACGTTCTCCTTCTACCTCTCCAACATCGGCCGCGACAACCCCCAGGGCAGCTTCGACTGC ATCCAGCAGTATGTCTCCAGTCATGGGGAAGTTCACCTGGACTGCCTGGGCAGCATACAG GACAAGATCACGGTGTGTGCCACCGACGACTCCTACCAGAAGGCGCGGCAGAGCATGGCC CAGGCGGAGGAGGAGACGCGGAGCCGAAGTGCCATTGTCATCAAGGCTGGAGGCCGCTAC CTGGGCAAGAAGGTTCAGTTTCGGAAACCAGCCCCAGGTGCAACAGACGCGGTGCCCTCC CGGAAGCGGGCAACCCCCATCAACTTGGCGAGTGCCATCANGAAGAGTGGTGCCAGTGCC GTGAGTGGGGGCAGCGGGGTGTCCCAGAAGCCCTTCCGTGACCGAGTGCTGCACCTCCTG GCACTACNGCCCTACCGCAAGGCTGAGCTGCTGCTGCGACTGCAGAAGGACNGCCTGACG CANGCNNGACAGGACGCGCTGGATGGCCNTCTCCCAGCAGTGGCCAACATGAGTGCTAAG GACNGCACGTGTACACTGGCAGACTGCATGTACAAGGATGTGCANAAGGACTGGCCCTGN CTACTCGNAGGGGACANNCACTGCTGAANCGGNNTGCTCGTCCGAAGCTGTGCCAGCACA >OriGene 3' read for NM_006532 unedited
CTANGTTCNGTTTTTTTTTTTTTTTTTTTTAACAAAAAATTTAACCCTTTTTAAACAACT TAGAACTTTGGCCCCCAACCCCCAAAACAAAAAAATTTTCTTAACTTCATAATTCCCTAA AAGGGGAACCAATTAACCACCAAAACCACCACCACCACCAAAAGGGCAATTCAACTGGCC ACCCCTTGGACCGGTTCCCAAGGCAAAAGGGCCGGCAATCCCGGGCTGGGACCCCCCGAA ATCATCCCACCCTACCCGGGTTTTGAAGGCTTCCCGGGGGCCAAACAACAAGGGCCAACT TACCTTGGAAGCCCCCTGGAACCCGGTTGGGCCTTTCCCGGGCCGGCCCGGGCCAAAAAA AAAAACCACCACCGCTTTTGGAACCTTGAAGATCCATTTCCCCGGAACCTTGGGAACCCC GGGGGCCCAAAAGAAAAAAACCCTTGGGCCAGGGGGGGATACCCCGGGGGCCCCCCACAA TCAACTCTTTGGAAACCTGCAAGAAGCCCCCCTCCACCCCCCAATTTAAAAAAATAAACA TCTGGTTTTTTGCCTTTTTGGTCCCCCCGGCCGACTGGTTGTGGCAACCATGACCCCCAT GGGATCCCTGGGCCCTGGATTACAAGCCGCATTCAACCCCCAACAACTTTCCAAATCATG GGTTCATTCAACCCTTAATTGGAACCCCCACCCTTAAACCCCACCCTGCCCTGGGGGGGT CCCCAAATAACCCAACTACACAACCACAATTTGGTTGCCCCCCGATGCCTGGGGGGGACC CTAGACTAGGGCCACAGCACCGAACCACCATTGGGGTGGCCTGCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006532 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006532.2, NP_006523.1 |
| RefSeq Size | 4058 bp |
| RefSeq ORF | 1866 bp |
| Locus ID | 8178 |
| UniProt ID | P55199 |
| Protein Families | Transcription Factors |
| Gene Summary | Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed:23932780). ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208258 | ELL (Myc-DDK-tagged)-Human elongation factor RNA polymerase II (ELL) |
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|
| RC208258L1 | Lenti ORF clone of Human elongation factor RNA polymerase II (ELL), Myc-DDK-tagged |
CNY 9336.00 |
|
| RC208258L2 | Lenti ORF clone of Human elongation factor RNA polymerase II (ELL), mGFP tagged |
CNY 9336.00 |
|
| RC208258L3 | Lenti ORF clone of Human elongation factor RNA polymerase II (ELL), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC208258L4 | Lenti ORF clone of Human elongation factor RNA polymerase II (ELL), mGFP tagged |
CNY 5890.00 |
|
| RG208258 | ELL (tGFP-tagged) - Human elongation factor RNA polymerase II (ELL) |
CNY 8536.00 |
