PDE2A (NM_002599) Human Untagged Clone

Name: PDE2A (NM_002599) Human Untagged Clone
SKU: SC110970
Price: 6896.00

CAT＃: SC110970

PDE2A (untagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1

Datasheet

SDS

"NM_002599" in other vectors (6)

CNY 6896.00

货期*

现货

规格

Cited in 1 publication.

Product images

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PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

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Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CGS-PDE; cGSPDE; PDE2A1; PED2A4
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for NM_002599 edited ATGGGGCAGGCATGCGGCCACTCCATCCTCTGCAGGAGCCAGCAGTACCCGGCAGCGCGA CCGGCTGAGCCGCGGGGCCAGCAGGTCTTCCTCAAGCCGGACGAGCCGCCGCCGCCGCCG CAGCCATGCGCCGACAGCCTGCAGGACGCCTTGCTGAGTCTGGGCTCTGTCATCGACATT TCAGGCCTGCAACGTGCTGTCAAGGAGGCCCTGTCAGCTGTGCTCCCCCGAGTGGAAACT GTCTACACCTACCTACTGGATGGTGAGTCCCAGCTGGTGTGTGAGGACCCCCCACATGAG CTGCCCCAGGAGGGGAAAGTCCGGGAGGCTATCATCTCCCAGAAGCGGCTGGGCTGCAAT GGGCTGGGCTTCTCAGACCTGCCAGGGAAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCT CCTGATACCCAAGTGCTGGTCATGCCGCTAGCGGACAAGGAGGCTGGGGCCGTGGCAGCT GTCATCTTGGTGCACTGTGGCCAGCTGAGTGATAATGAGGAATGGAGCCTGCAGGCGGTG GAGAAGCATACCCTGGTCGCCCTGCGGAGGGTGCAGGTCCTGCAGCAGCGCGGGCCCAGG GAGGCTCCCCGAGCCGTCCAGAACCCCCCGGAGGGGACGGCGGAAGACCAGAAGGGCGGG GCGGCGTACACCGACCGCGACCGCAAGATCCTCCAACTGTGCGGGGAACTCTACGACCTG GATGCCTCTTCCCTGCAGCTCAAAGTGCTCCAATACCTGCAGCAGGAGACCCGGGCATCC CGCTGCTGCCTCCTGCTGGTGTCGGAGGACAATCTCCAGCTTTCTTGCAAGGTCATCGGA GACAAAGTGCTCGGGGAAGAGGTCAGCTTTCCCTTGACAGGATGCCTGGGCCAGGTGGTG GAAGACAAGAAGTCCATCCAGCTGAAGGACCTCACCTCCGAGGATGTACAACAGCTGCAG AGCATGTTGGGCTGTGAGCTGCAGGCCATGCTCTGTGTCCCTGTCATCAGCCGGGCCACT GACCAGGTGGTGGCCTTGGCCTGCGCCTTCAACAAGCTAGAAGGAGACTTGTTCACCGAC GAGGACGAGCATGTGATCCAGCACTGCTTCCACTACACCAGCACCGTGCTCACCAGCACC CTGGCCTTCCAGAAGGAACAGAAACTCAAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGCA AAGAACCTCTTCACCCACCTGGATGACGTCTCTGTCCTGCTCCAGGAGATCATCACGGAG GCCAGAAACCTCAGCAACGCAGAGATCTGCTCTGTGTTCCTGCTGGATCAGAATGAGCTG GTGGCCAAGGTGTTCGACGGGGGCGTGGTGGATGATGAGAGCTATGAGATCCGCATCCCG GCCGATCAGGGCATCGCGGGACACGTGGCGACCACGGGCCAGATCCTGAACATCCCTGAC GCATATGCCCATCCGCTTTTCTACCGCGGCGTGGACGACAGCACCGGCTTCCGCACGCGC AACATCCTCTGCTTCCCCATCAAGAACGAGAACCAGGAGGTCATCGGTGTGGCCGAGCTG GTGAACAAGATCAATGGGCCATGGTTCAGCAAGTTCGACGAGGACCTGGCGACGGCCTTC TCCATCTACTGCGGCATCAGCATCGCCCATTCTCTCCTATACAAAAAAGTGAATGAGGCT CAGTATCGCAGCCACCTGGCCAATGAGATGATGATGTACCACATGAAGGTCTCCGACGAT GAGTATACCAAACTTCTCCATGATGGGATCCAGCCTGTGGCTGCCATTGACTCCAATTTT GCAAGTTTCACCTATACCCCTCGTTCCCTGCCCGAGGATGACACGTCCATGGCCATCCTG AGCATGCTGCAGGACATGAATTTCATCAACAACTACAAAATTGACTGCCCGACCCTGGCC CGGTTCTGTTTGATGGTGAAGAAGGGCTACCGGGATCCCCCCTACCACAACTGGATGCAC GCCTTTTCTGTCTCCCACTTCTGCTACCTGCTCTACAAGAACCTGGAGCTCACCAACTAC CTCGAGGACATCGAGATCTTTGCCTTGTTTATTTCCTGCATGTGTCATGACCTGGACCAC AGAGGCACAAACAACTCTTTCCAGGTGGCCTCGAAATCTGTGCTGGCTGCGCTCTACAGC TCTGAGGGCTCCGTCATGGAGAGGCACCACTTTGCTCAGGCCATCGCCATCCTCAACACC CACGGCTGCAACATCTTTGATCATTTCTCCCGGAAGGACTATCAGCGCATGCTGGATCTG ATGCGGGACATCATCTTGGCCACAGACCTGGCCCACCATCTCCGCATCTTCAAGGACCTC CAGAAGATGGCTGAGGTGGGCTACGACCGAAACAACAAGCAGCACCACAGACTTCTCCTC TGCCTCCTCATGACCTCCTGTGACCTCTCTGACCAGACCAAGGGCTGGAAGACTACGAGA AAGATCGCGGAGCTGATCTACAAAGAATTCTTCTCCCAGGGAGACCTGGAGAAGGCCATG GGCAACAGGCCGATGGAGATGATGGACCGGGAGAAGGCCTATATCCCTGAGCTGCAAATC AGCTTCATGGAGCACATTGCAATGCCCATCTACAAGCTGTTGCAGGACCTGTTCCCCAAA GCGGCAGAGCTGTACGAGCGCGTGGCCTCCAACCGTGAGCACTGGACCAAGGTGTCCCAC AAGTTCACCATCCGCGGCCTCCCAAGTAACAACTCGCTGGACTTCCTGGATGAGGAGTAC GAGGTGCCTGATCTGGATGGCACTAGGGCCCCCATCAATGGCTGCTGCAGCCTTGATGCT GAGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_002599 unedited TCGCTGAGGAGCGCATTTCAGTGGCAGCGGGNAGAGGCGGGAGCAGCGGGGCAGCATAGC TGGATTGGGGTGTTGAGTCCAGGCTGAGTAGGGGGCAGCCCACTGCTCTTGGTCCCTGTG CCTGCTGGGGGTGCCCTGCCCTGAACTCCAGGCAGCGGGGACAGGGCGATGTGCCACCTT AGTCTGGCTGGGGAGGCGGACGATGAGGAGTGATGGGGCAGGCATGCGGCCACTCCATCC TCTGCAGGAGCCAGCAGTACCCGGCAGCGCGACCGGCTGAGCCGCGGGGCCAGCAGGTCT TCCTCAAGCCGGACGAGCCGCCGCCGCCGCCGCAGCCATGCGCCGACAGCCTGCAGGACG CCTTGCTGAGTCTGGGCTCTGTCATCGACATTTCAGGCCTGCAACGTGCTGTCAAGGAGG CCCTGTCAGCTGTGCTCCCCCGAGTGGAAACTGTCTACACCTACCTACTGGATGGTGAGT CCCAGCTGGTGTGTGAGGACCCCCCACATGAGCTGCCCCAGGAGGGGAAAGTCCGGGAGG CTATCATCTCCCATAAGCGGCTGGGCTGCAATGGGCTGGGCTTCTCAGACCTGCCAGGGA AGCCCTTGGCCAGGCTGGTGGCTCCACTGGCTCCTGATACCCAAGTGCTGGTCATGCCGC TAGCGGACAAGGAGGCTGGGGCCGTGGCAGCTGTCATCCTTGTGCACTGTGGCCAGCTGG ATGATAATGAGGAATGGAGCCCTGCAGCGGTGGAGAAGCATACCCTGGTCGCCCTTGCGG AGGTGCAGGTCCTGCAGCACCGCGGCCCCAGGGAGGTCTCCCGAACCGTCCAGAACCCCC GAAAGGGACT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_002599 unedited ATGGCCGCGGCCGCATCTATATCGAGTTTTTTTTTTTTTTTTTTGGTAGAAAGAAAAGAT TTATTCTCATGTACAAAGCGGTCAGCCCACGGGACCATATACGACAGTTGCACAGAGTCC TATAAAAACGCATCTCTCTAAAGGCAACTCAGAAAGGTAAGGCAGGTGGACCCCCTCCCC CACCCCACAACGCACACAGAATGAAACGGAGAAAAAGAGAGAAGCCAGTGGCCGGGCTGA CCCAAGAGTCCCGGCCCTATGGGGTCTCCCAAGCCCCAGGGCACAGGTGGATATGGCCTT GAAGAGAGAGCCCTGCCAGGGCTGAAGCCAGGTCTCTCACTGGCTGCAGGAATGGGTAAG GGGCTCAGGCCAAGGGGAACACTCAGGGGGCCTCTGAGACACTCAAGGTTGGGCTCTCTG TGCCTTAAGGTTGCAGAAACATTCCATAGCCTCTGCTGGGACTCCGCAGAGCGATAGAGC TATTTTAGACATTGATCTAAAATGGATCTTATAGCATGGATTGTCTTACCCGCTCCCTGT TTGGCCCAGACAGGGGAAGAGACTCGCCCAGCGTCACACAGGCAGGAGGAAGCATGGCTG GCTCATTCATACCTGNGCCTGTCTCCAGTGCGGNGATCCCTNCTGCCCTCAATCCATCCT GCTTCTCCCAGGGCCCCTCTCTGCAGACACTGAGCTGCCCTCTGCAGCTCTTCCCTGGGC CCCTGATCCCCCAGTCCCATCCACATATTTAATAAGTCCCTCAAAGGGGCTTCGCCCCGG GTGTTTTTAGTCTCCCAGATAAGGAATGTTTCCAGATGCCACTTGTACTCAAATGCTACC TCCTCCTAAAGATCCGAACCCCAGGGGGGGAACCCAAAGGATGACAATATTGGGGGCAAA TGGGCAGAAACCTCACGCAAAAAAGCTGGNAATGCCAGGGGCCAGAGCCAATAGC
Restriction Sites	NotI-NotI
ACCN	NM_002599
Insert Size	4600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_002599.1, NP_002590.1
RefSeq Size	4240 bp
RefSeq ORF	2826 bp
Locus ID	5138
UniProt ID	O00408
Domains	PDEase, GAF, HDc
Protein Families	Druggable Genome
Protein Pathways	Progesterone-mediated oocyte maturation, Purine metabolism
Gene Summary	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line) (PubMed:24705027).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform, PDE2A3 (see PMID:9210593). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Optical Mapping of cAMP Signaling at the Nanometer Scale. ,null, Cell ,PubMed ID 32846156 [PDE2A]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC207219	PDE2A (Myc-DDK-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 6896.00 CNY 7130.00
RC207219L1	Lenti ORF clone of Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9296.00
RC207219L2	Lenti ORF clone of Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9030.00
RC207219L3	Lenti ORF clone of Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9030.00
RC207219L4	Lenti ORF clone of Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9030.00
RG207219	PDE2A (tGFP-tagged) - Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 8496.00

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