GLE1 (NM_001499) Human Untagged Clone
CAT＃: SC110907
GLE1 (untagged)-Human GLE1 RNA export mediator homolog (yeast) (GLE1), transcript variant 2
CNY 4912.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAAHC; CAAHD; GLE1L; hGLE1; LCCS; LCCS1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001499, the custom clone sequence may differ by one or more nucleotides
ATGCCGTCTGAGGGTCGCTGCTGGGAGACCTTGAAGGCCCTACGCAGTTCCGACAAAGGTCGCCTTTGCT ACTACCGCGACTGGCTGCTGCGGCGCGAGGATGTTTTAGAAGAATGTATGTCTCTTCCCAAGCTATCTTC TTATTCTGGATGGGTGGTAGAGCACGTCCTACCCCATATGCAGGAGAACCAACCTCTGTCTGAGACTTCG CCATCCTCTACGTCAGCTTCAGCCCTAGATCAACCCTCATTTGTTCCCAAATCTCCTGACGCAAGCTCTG CCTTTTCCCCAGCCTCCCCTGCAACACCAAATGGAACCAAGGGCAAAGATGAGTCCCAGCACACAGAATC TATGGTACTTCAGTCCTCACGGGGGATCAAAGTGGAAGGCTGCGTCCGAATGTACGAACTGGTACACAGA ATGAAAGGAACAGAGGGCCTGAGGCTATGGCAGGAGGAGCAGGAGAGGAAGGTGCAAGCCCTCTCGGAGA TGGCATCTGAACAACTGAAGCGGTTTGATGAATGGAAGGAACTGAAGCAGCATAAAGAATTCCAGGACTT GCGGGAAGTAATGGAGAAGAGCTCCAGAGAAGCCTTGGGACACCAAGAGAAGCTAAAAGCTGAGCACCGT CACAGAGCAAAGATTCTCAACCTGAAGCTGCGGGAAGCAGAGCAGCAGCGCGTGAAGCAAGCAGAACAGG AGCGGCTTCGGAAGGAAGAAGGCCAGATCCGCCTGCGGGCCCTCTATGCTCTGCAGGAGGAGATGCTGCA GCTCAGCCAGCAGCTGGATGCCTCTGAGCAGCACAAAGCCCTGCTTAAGGTCGACCTGGCTGCCTTCCAG ACCCGAGGCAACCAGCTGTGCAGCCTCATCTCAGGGATCATCCGGGCCTCTTCAGAGAGCAGCTATCCCA CAGCAGAGAGTCAAGCTGAGGCTGAGCGAGCTCTGCGGGAAATGCGGGACCTCCTGATGAACTTGGGGCA GGAGATCACCAGAGCCTGCGAAGACAAGAGGAGGCAGGATGAAGAAGAGGCCCAGGTAAAGCTGCAAGAG GCACAGATGCAGCAGGGACCAGAGGCCCACAAAGAGCCCCCAGCTCCCAGCCAGGGCCCAGGAGGGAAAC AGAATGAAGACCTCCAGGTGAAGGTACAAGACATTACAATGCAGTGGTACCAGCAGCTGCAGGATGCTTC CATGCAGTGTGTGTTGACCTTTGAGGGCCTGACCAACAGCAAGGACAGTCAGGCCAAAAAGATAAAGATG GACCTCCAGAAGGCTGCTACCATCCCAGTGAGCCAAATCTCTACCATTGCAGGCTCAAAACTGAAGGAGA TCTTTGACAAGATCCACAGCCTGCTCTCTGGAAAACCTGTTCAATCTGGTGGGCGCTCTGTGTCTGTCAC ACTTAACCCACAGGGGCTGGACTTTGTTCAATACAAACTGGCAGAGAAATTTGTGAAACAAGGCGAGGAG GAAGTGGCCTCTCACCATGAAGCAGCATTCCCCATTGCAGTTGTGGCATCCGGGATCTGGGAGCTCCACC CCAGAGTGGGGGACCTCATTCTTGCTCATCTACATAAGAAGTGTCCTTACTCTGTTCCTTTCTATCCCAC TTTCAAGGAGGGAATGGCTTTGGAAGACTATCAGAGGATGCTTGGTTACCAAGTAAAGGATTCCAAAGTG GAGCAGCAAGACAACTTTCTAAAACGCATGTCAGGGATGATCCGTCTCTACGCTGCTATCATCCAGCTCC GGTGGCCATATGGAAACCGACAGGAGATTCACCCTCATGGCTTAAATCATGGATGGCGCTGGTTGGCACA GATCTTAAACATGGAGCCCTTGTCAGATGTGACAGCCACCCTCCTCTTTGACTTCCTGGAGGTGTGTGGG AATGCCCTCATGAAGCAATACCAGGTTCAGTTCTGGAAGATGCTAATTCTCATCAAAGAGGACTACTTTC CCAGGTATCAGGCTTGTTGA >OriGene 5' read for NM_001499 unedited
TATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCCTGTGTGGCCT TCCCGGCGGCTGATTCGAGGGCTTGTTTGGTCAGAAGGGGGGCGTCAGAGAAGCTGCCCC TTAGCCAACCATGCCGTCTGAGGGTCGCTGCTGGGAGACCTTGAAGGCCCTACGCAGTTC CGACAAAGGTCGCCTTTGCTACTACCGCGACTGGCTGCTGCGGCGCGAGGATGTTTTAGA AGAATGTATGTCTCTTCCCAAGCTATCTTCTTATTCTGGATGGGTGGTAGAGCACGTCCT ACCCCATATGCAGGAGAACCAACCTCTGTCTGAGACTTCGCCATCCTCTACGTCAGCTTC AGCCCTAGATCAACCCTCATTTGTTCCCAAATCTCCTGACGCAAGCTCTGCCTTTTCCCC AGCCTCCCCTGCAACACCAAATGGAACCAAGGGCAAAGATGAGTCCCAGCACACAGAATC TATGGTACTTCAGTCCTCACGGGGGATCAAAGTGGAAGGCTGCGTCCGAATGTACGAACT GGTACACAGAATGAAAGGAACAGAGGGCCTGAGGCTATGGCAGGAGGAGCAGGAGAGGAA GGTGCAAGCCCTCTCGGAGATGGCATCTGAACAACTGAAGCGGTTTGATGAATGGAAGGA ACTGAAGCAGCATAAAGAATTCCAGGACTTGCGGGAAGTAATGGAGAAGAGCTCCAGAGA AGCCTTGNGACACCAACTAGAGCTAANAGCTGAGCACCGTCACAGAGCAAAGATTCTCAA CCTGAAGCTGCGGGAAGCAGAGCAGCAGCGCGTGAAGCAAGCAGAACAAGAGCGGGCTTC GAAGGAAGAAAGCCAGTCGCCTGCGGCCCTCTTGCTCTGCAGAGAGATGCTCACTCACCA GCACTGATGCTCTGACACACAAGCTGCTAGGTGACTGGTGCTTCAGACCGAGCACAGTGG CANCTATTTAGGATCATCGGCTCTCAAA >OriGene 3' read for NM_001499 unedited
NNGGGGTAAGACTAGNAACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTTTT CAAAAAACCAAGCTTTATTATTAATTTTTTTTGGCACCTTAATTAACAATTCATAGAATG GGTCACTTCAGGCCACTCAAGAGTACCAGTGAACACTCCCCCACAAACACACCCTGCCAC AAGACATTTAGCACAGAGGAACAGATCCATGGCCACTGCCTCTGCAGTATCAAAGAGAAT TAGTCTTTCCACAAAACAAATTTTAACAGCCAATCTCTGGATTTCTGTAGTGGCTTTAGT CAGGCATATTTATCATCATATTAGCAGTGTTCAGTTCCTGCCCAACATCTTTATTTAATC CCAATTCAATGCTTATGGATGCTCAGCTCATGTTTAATGTTGCAAGCCCCATCTTAGCCC ATCTTAATTCAAACAGAAAAGAAACAAAACAAAACAAAAACAAAAAAGGTACCTGCCTGG TTCATGGATCCCTAGCCATCCAGGGACCAAATTCCAAATTAGGATGACAAAGAGTTTCCC TTAGTTCAAAATGACATGTGTTTCCAGTCCTAATCCCAGATGTTAATCTAGCCATAGTGT CACCTGAGCCTTAGTCCATGTGTATATCACATACTTCCTCTAGCTTTAGAATGACTTCAC AAGCTGAGAAAGGCAAAACAACCATAACCCAGACTTGCATGTAAGTATCTGGCCTCAACT ATTAAATAATACTTAAAAGGGAACCTTATGAGCCTTAGCTCTTTCTGGAAATTCATCTGA CAGGGTGAGGTGNTNANNTGGGGGGTCCAAAAATGGGTGGCCTCCCTCCACCTCAGAGTG CATTTGTTAAGGTATTGCCCACTTTCTGAAAGACACTAGAGACCAGGGAGGAGACCAAGT GCTTAAGAACCTTTATCACTTTTGCAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001499 |
| Insert Size | 3500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001499.2, NP_001490.1 |
| RefSeq Size | 2266 bp |
| RefSeq ORF | 1980 bp |
| Locus ID | 2733 |
| UniProt ID | Q53GS7 |
| Gene Summary | This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) has an alternate 3' sequence, as compared to variant 1. The encoded isoform 2 has a shorter and distinct C-terminus, as compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219925 | GLE1 (Myc-DDK-tagged)-Human GLE1 RNA export mediator homolog (yeast) (GLE1), transcript variant 2 |
CNY 4560.00 |
|
| RC219925L3 | Lenti-ORF clone of GLE1 (Myc-DDK-tagged)-Human GLE1 RNA export mediator homolog (yeast) (GLE1), transcript variant 2 |
CNY 6460.00 |
|
| RC219925L4 | Lenti-ORF clone of GLE1 (mGFP-tagged)-Human GLE1 RNA export mediator homolog (yeast) (GLE1), transcript variant 2 |
CNY 6460.00 |
|
| RG219925 | GLE1 (tGFP-tagged) - Human GLE1 RNA export mediator homolog (yeast) (GLE1), transcript variant 2 |
CNY 5040.00 |
