WAC (NM_100264) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BM-016; DESSH; PRO1741; Wwp4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_100264 edited GAATTCGGCACGAGGCTCCCCCCTCCCCGACACACACTCACAGGCCGGGCATTGATGGTA ATGTATGCGAGGAAACAGCAGAGACTCAGTGATGGCTGTCACGACCGGAGGGGGGACTCG CAGCCTTACCAGGCACTTAAGTATTCATCGAAGAGTCACCCCAGTAGCGGTGATCACAGA CATGAAAAGATGCGAGACGCCGGAGATCCTTCACCACCAAATAAAATGTTGCGGAGATCT GATAGTCCTGAAAACAAATACAGTGACAGCACAGGTCACAGTAAGGCCAAAAATGTGCAT ACTCACAGAGTTAGAGAGAGGGATGGTGGGACCAGTTACTCTCCACAAGAAAATTCACAC AACCACAGTGCTCTTCATAGTTCAAATTCACATTCTTCTAATCCAAGCAATAACCCAAGC AAAACTTCAGATGCACCTTATGATTCTGCAGATGACTGGTCTGAGCATATTAGCTCTTCT GGGAAAAAGTACTACTACAATTGTCGAACAGAAGTTTCACAATGGGAAAAACCAAAAGAG TGGCTTGAAAGAGAACAGAGACAAAAAGAAGCAAACAAGATGGCAGTCAACAGCTTCCCA AAAGATAGGGATTACAGAAGAGAGGTGATGCAAGCAACAGCCACTAGTGGGTTTGCCAGT GGAATGGAAGACAAGCATTCCAGTGATGCCAGTAGTTTGCTCCCACAGAATATTTTGTCT CAAACAAGCAGACACAATGACAGAGACTACAGACTGCCAAGAGCAGAGACTCACAGTAGT TCTACGCCAGTACAGCACCCCATCAAACCAGTGGTTCATCCAACTGCTACCCCAAGCACT GTTCCTTCTAGTCCATTTACGCTACAGTCTGATCACCAGCCAAAGAAATCATTTGATGCT AATGGAGCATCTACTTTATCAAAACTGCCTACACCCACATCTTCTGTCCCTGCACAGAAA ACAGAAAGAAAAGAATCTACATCAGGAGACAAACCCGTATCACATTCTTGCACAACTCCT TCCACGTCTTCTGCCTCTGGACTGAACCCCACATCTGCACCTCCAACATCTGCTTCAGCG GTCCCTGTTTCTCCTGTTCCACAGTCGCCAATACCTCCCTTACTTCAGGACCCAAATCTT CTTAGACAATTGCTTCCTGCTTTGCAAGCCACGCTGCAGCTTAATAATTCTAATGTGGAC ATATCTAAAATAAATGAAGTTCTTACAGCAGCTGTGACACAAGCCTCACTGCAGTCTATA ATTCATAAGTTTCTTACTGCTGGACCATCTGCTTTCAACATAACGTCTCTGATTTCTCAA GCTGCTCAGCTCTCTACACAAGCCCAGCCATCTAATCAGTCTCCGATGTCTTTAACATCT GATGCGTCATCCCCAAGATCATATGTTTCTCCAAGAATAAGCACACCTCAAACTAACACA GTCCCTATCAAACCTTTGATCAGTACTCCTCCTGTTTCATCACAGCCAAAGGTTAGTACT CCAGTAGTTAAGCAAGGACCAGTGTCACAGTCAGCCACACAGCAGCCTGTAACTGCTGAC AAGCAGCAAGGTCATGAACCTGTCTCTCCTCGAAGTCTTCAGCGCTCAAGTAGCCAGAGA AGTCCATCACCTGGTCCCAATCATACTTCTAATAGTAGTAATGCATCAAATGCAACAGTT GTACCACAGAATTCTTCTGCCCGATCCACGTGTTCATTAACGCCTGCACTAGCAGCACAC TTCAGTGAAAATCTCATAAAACACGTTCAAGGATGGCCTGCAGATCATGCAGAGAAGCAG GCATCAAGATTACGCGAAGAAGCGCATAACATGGGAACTATTCACATGTCCGAAATTTGT ACTGAATTAAAAAATTTAAGATCTTTAGTCCGAGTATGTGAAATTCAAGCAACTTTGCGA GAGCAAAGGATACTATTTTTGAGACAACAAATTAAGGAACTTGAAAAGCTAAAAAATCAG AATTCCTTCATGGTGTGAAGATGTGAATAATTGCACATGGTTTTGAGAACAGGAACTGTA AATCTGTTGCCCAATCTTAACATTTTTGAGCTGCATTTAAGTAGACTTTGGACCGTTAAG CTGGGCAAAGGAAATGACAAGGGGACGGGGTCTGTGAGAGTCAATTCAGGGGAAAGATAC AAGATTGATTTGTAAAACCCTTGAAATGTAGATTTCTTGTAGATGTATCCTTCACGTTGT AAATATGTTTTGTAGAGTGAAGCCATGGGAAGCCATGTGTAACAGAGCTTAGACATCCAA AACTAATCAATGCTGAGGTGGCTAAATACCTAGCCTTTTACATGTAAACCTGTCTGCAAA ATTAGCTTTTTTAAAAAAAAAAAAAAAAAACTCGAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_100264 unedited GTTGGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTCCCCC CTCCCCGACACACACTCACAGGCCGGGCATTGATGGTAATGTATGCGAGGAAACAGCAGA GACTCAGTGATGGCTGTCACGACCGGAGGGGGGACTCGCAGCCTTACCAGGCACTTAAGT ATTCATCGAAGAGTCACCCCAGTAGCGGTGATCACAGACATGAAAAGATGCGAGACGCCG GAGATCCTTCACCACCAAATAAAATGTTGCGGAGATCTGATAGTCCTGAAAACAAATACA GTGACAGCACAGGTCACAGTAAGGCCAAAAATGTGCATACTCACAGAGTTAGAGAGAGGG ATGGTGGGACCAGTTACTCTCCACAAGAAAATTCACACAACCACAGTGCTCTTCATAGTT CAAATTCACATTCTTCTAATCCAAGCAATAACCCAAGCAAAACTTCAGATGCACCTTATG ATTCTGCAGATGACTGGTCTGAGCATATTAGCTCTTCTGGGAAAAAGTACTACTACAATT GTCGAACAGAAGTTTCACAATGGGAAAAACCAAAAGAGTGGCTTGAAAGAGAACAGAGAC AAAAAGAAGCAAACAAGATGGCAGTCAACAGCTTCCCAAAAGATAGGGATTACAGAAGAG AGGTGATGCAAGCAACAGCCACTAGTGGGTTTTGCCAGTGGAATGGAAAGACAGCATTCC AGTGATGCCAGTAGTTTGCTCCCACAGAATATTNTGTCTCANNACAGCAGACACCATGAC AGAGACTACAGACTGCCAAGAGCAGAGACTCACAGTAGTTCTACGCCAGTACAGCACCCC ATCAAACCAGTGGTNTCATCCAACTGCTACCCCAGCACTGNTCCTTCTAGTCCATTTACG CTACAGTCTGATCACCAGCCAAGAAATCATTTGATGCTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_100264 unedited CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAAAACTAATTTTGCAGACA GGTTTACATGTAAAAGGCTAGGTATTTAGCCACCTCAGCATTGATTAGTTTTGGATGTCT AAGCTCTGTTACACATGGCTTCCCATGGCTTCACTCTACAAAACATATTTACAACGTGAA GGATACATCTACAAGAAATCTACATTTCAAGGGTTTTACAAATCAATCTTGTATCTTTCC CCTGAATTGACTCTCACAGACCCCGTCCCCTTGTCATTTCCTTTGCCCAGCTTAACGGTC CAAAGTCTACTTAAATGCAGCTCAAAAATGTTAAGATTGGGCAACAGATTTACAGTTCCT GTTCTCAAAACCATGTGCAATTATTCACATCTTCACACCATGAAGGAATTCTGATTTTTT AGCTTTTCAAGTTCCTTAATTTGTTGTCTCAAAAATAGTATCCTTTGCTCTCGCAAAGTT GCTTGAATTTCACATACTCGGACTAAAGATCTTAAATTTTTTAATTCAGTACAAATTTCG GACATGTGAATAGTTCCCATGTTATGCGCTTCTTCGCGTAATCTTGATGCCTGCTTCTCT GCATGATCTGCAGGCCATCCTTGAACGTGTTTTATGAGATTTTCACTGAAGTGTGCTGCT AGTGCAGGCGTTAATGAACACGTGGATCGGGCAGAAGAATTCTGTGGTACAACTGTTGCA TTTGATGCATTACTACTATTANAAGTATGATTGGGACCAGGTGATGGACTTCTCTGGGCT ACTGAGCGCTGAAGACTTCGAGGAGAGACAGGTTCATGACCTTGCTGNCTGTCAGCAGNT ACAGGCTGCTGNGTGGCTGACTGTGACACTGGTCCTTGCTTTACTACTGGAGTACTAACC CTTGGCTGGGATGAACGGAAGAGTACTGATCAAGGTTGAAGGGACGGGTTATTTAAGGGT GCTATCTTGAAAACTATGACTG
Restriction Sites	NotI-NotI
ACCN	NM_100264
Insert Size	2440 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_100264.1, NP_567822.1
RefSeq Size	3088 bp
RefSeq ORF	1809 bp
Locus ID	51322
UniProt ID	Q9BTA9
Domains	WW
Gene Summary	The protein encoded by this gene contains a WW domain, which is a protein module found in a wide range of signaling proteins. This domain mediates protein-protein interactions and binds proteins containing short linear peptide motifs that are proline-rich or contain at least one proline. This gene product shares 94% sequence identity with the WAC protein in mouse, however, its exact function is not known. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008] Transcript Variant: This variant (2) contains an alternate 5' terminal exon, and it thus differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, comapared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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