RAD17 (NM_133339) Human Untagged Clone
CAT＃: SC109696
RAD17 (untagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 2
CNY 5000.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCYC; HRAD17; R24L; RAD17SP; RAD24 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_133339, the custom clone sequence may differ by one or more nucleotides
ATGTCAAAAACTTTTCTTAGACCAAAGGTATCTTCCACAAAGGTAACAGACTGGGTTGACCCATCATTTG ATGATTTTCTAGAGTGTAGTGGCGTCTCTACTATTACTGCCACATCATTAGGTGTGAATAACTCAAGTCA TAGAAGAAAAAATGGGCCTTCTACATTAGAAAGCAGCAGATTTCCAGCGAGAAAAAGAGGAAATCTATCT TCCTTAGAACAGATTTATGGTTTAGAAAATTCAAAAGAATATCTGTCTGAAAATGAACCATGGGTGGATA AATATAAACCAGAAACTCAGCATGAACTTGCTGTGCATAAAAAGAAAATTGAAGAAGTCGAAACCTGGTT AAAAGCTCAAGTTTTAGAAAGGCAACCAAAACAGGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGA TGTGGAAAGACAACGACCTTAAAAATACTATCAAAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATC CAGTTTTACCAGACTTCCAAAAAGATGATTTCAAGGGGATGTTTAATACTGAATCAAGCTTCCATATGTT TCCCTATCAGTCTCAGATAGCAGTTTTCAAAGAGTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAA ATGCTTGGAGATGATCTGAGAACTGATAAGAAGATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATC GGGATTCTCATACTTTACATGAAGTTCTAAGGAAGTATGTGAGGATTGGTCGATGTCCTCTTATATTTAT AATCTCGGACAGTCTCAGTGGAGATAATAATCAAAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGT TCTATCTCAAATATTAGTTTCAACCCTGTGGCACCAACAATTATGATGAAATTTCTTAATCGAATAGTGA CTATAGAAGCTAACAAGAATGGAGGAAAAATTACTGTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCA GGGATGTTCTGGTGATATCAGAAGTGCAATAAACAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAAC TTACGGCCAAGGAAAAAAGGAATGTCTTTAAAATCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAAA AACCTGATAGGGTTTTTGAAAATCAAGAGGTCCAAGCTATTGGTGGCAAAGATGTTTCTCTGTTTCTCTT CAGAGCTTTGGGGAAAATTCTATATTGTAAAAGAGCATCTTTAACAGAATTAGACTCACCTCGGTTGCCC TCTCATTTATCAGAATATGAACGGGATACATTACTTGTTGAACCTGAGGAGGTAGTAGAAATGTCACACA TGCCTGGAGACTTATTTAATTTATATCTTCACCAAAACTACATAGATTTCTTCATGGAAATTGATGATAT TGTGAGAGCCAGTGAATTTCTGAGTTTTGCAGATATCCTCAGTGGTGACTGGAATACACGCTCTTTACTC AGGGAATATAGCACATCTATAGCTACGAGAGGTGTGATGCATTCCAACAAAGCCCGAGGATATGCTCATT GCCAAGGAGGAGGATCAAGTTTTCGACCCTTGCACAAACCTCAGTGGTTTCTAATAAATAAAAAGTATCG GGAAAATTGCCTGGCAGCAAAAGCACTTTTTCCTGACTTCTGCCTACCAGCTTTATGCCTCCAAACTCAG CTATTGCCATACCTTGCTCTACTAACCATTCCAATGAGAAATCAAGCTCAGATTTCTTTTATCCAAGATA TTGGAAGGCTCCCTCTGAAGCGACACTTTGGAAGATTGAAAATGGAAGCCCTGACTGACAGGGAACATGG AATGATAGACCCTGACAGCGGAGATGAAGCCCAGCTTAATGGAGGACATTCTGCAGAGGAATCTCTGGGT GAACCCACTCAAGCCACTGTGCCGGAAACCTGGTCTCTTCCTTTGAGTCAGAATAGTGCCAGTGAACTGC CTGCTAGCCAGCCCCAGCCCTTTTCAGCCCAAGGAGACATGGAAGAAAACATAATAATAGAAGACTACGA GAGTGATGGGACATAG >OriGene 5' read for NM_133339 unedited
NGCGCTTGTATTGTAAACGACTTACTATAGGCGGCCGCGAATTCGCACCAGGCGTTGAGC CCGGGTAGGGCCAGGTGGCTGCCCTTTCACCTAGGGTAGTCCCTGGTCGCCTCCGCTCTT CGCCCAAAAGGGATGCAGCTCCGGGAAACAAGTGAATTCATGGTATTTTACTTTTTTGGG AAATACTGGAAATGAAGACCTGCAACTGTTATTTGAAATAAGGAAAACTTTAATTTTCAG TATAAAAATTGCTCAAATAGAATTGCCTGATTTTAATGACAAAAGGTGAATTATAGTTTA ATGTACTGCAAGTCCTAAACTACGGATGGGAACTATTACAGTTTATAATGTCAAAAACTT TTCTTAGACCAAAGGTATCTTCCACAAAGTATATGGGAGTCCACATTTATGTAAGAAATG AAACTATAAAATGTATAAATAATTTGCAAATCAGAATTGCTGTCGAAAGTTTTACTATAA TGAAAGATATTTTCATACTCTCAAAAATATAGAGGAAAGGGGCCAAGATTATAGTACCAG TCACAATCTTTTGATGAGGACGAAATGAATCAGGTAACAGACTGGGTTGACCCATCATTT GATGATTTTCTAGAGTGTAGTGGCGTCTCTACTATTACTGCCACATCATTAGGTGTGAAT AACTCAAGTCATAGAAGAAAAAATGGGCCTTCTACATTAGAAAGCAGCAGATTTCCAGCG AGAAAAAGAGGAATCTATCTTCCTTAAAACAGATTTATGGTTTTAGAAAATCAAAAGAAT ATCTGTCTGAAAATGAACCATGGGTGGATAAATATAAACCAGAAACTCCGCATGAACTTG CTGTGCATAAAAAGAAATTGGAGAAGTCGAAACCTGGTTAAAGCTCAAGTTTAGAAAGCA ACC >OriGene 3' read for NM_133339 unedited
CNAGTAAAAACCTTTTTTTTTTAAATTCAACATTTTAATTGTGATATAACTTATGTAAAT ACTTTCCAAAAGTATCTAAGATATTCCACTGTACAACACATCAAAACTATCTGAGAAGCG GGAGACAATAAAGTGTAAATAAGGCACACAGTTTACACTCGTAATTGCATCCTAAACTGT TGAATAGTTGATATTTACAACCCCCCAATAACGTTTTTGCATCCTGAATTAGTCCTTCAC ATCTTGATATTTGGCATAATGTTCAAACACACTTTATACACACTGACCGCTCCTTAAACC ACTCACAGATTTAATAAATACCGAAAAAATTAAGAGATTTGAAGGCTTGATCTTCTATTT ACTTCAAGATGACAGAAGAGTAGGTTTCTTGTGATGAAATACTACAAGAATGAAGAATTA ACAAACTGTTGTGTAATTCATCAGAAAAGCATATTAACTCTGCTGAAGTACCACTGAATG AAACAAAAATGAAGCTGTGAAGTAGCAATCTGATTAGCAGGCTGGCTTCTATGTCCCATC ACTCTCGTAGTCTTCTATTATTATGTTTTCTTCCATGTCTCCTTGGGCTGAAAAGGGCTG GGGCTGGCTAGCAGGCAGTTCACTGGCACTATTCTGACTCAAAGGAAGAGACCAGGTTTC CGGCACAGTGGCTTGAGTGGGTTCACCCAGAGATTCCTCTGCAGAATGTCCTCCATTAAG CTGGGCTTCATCTCCGCTGTCAGGGTCTATCATTCCATGTTCCCTGTCAGTCAGGGCTTC CATTTTCAAATCTTCCAAAGTGTCGCTTCAGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_133339 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133339.1, NP_579917.1 |
| RefSeq Size | 2633 bp |
| RefSeq ORF | 2046 bp |
| Locus ID | 5884 |
| UniProt ID | O75943 |
| Domains | Rad17 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) lacks an internal exon in the 5' region, which results in an alternate upstream translation start codon, when compared to variant 1. The resulting protein (isoform 2) is longer and has a distinct N-terminus, as compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Efficient Herpes Simplex Virus 1 Replication Requires Cellular ATR Pathway Proteins
,Kareem N. Mohni, Alexander R. Dee, Samantha Smith, April J. Schumacher, and Sandra K. Weller,
J. Virol., Jan 2013; 87: 531 - 542.
[RAD17]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216025 | RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 2 |
CNY 7220.00 |
|
| RC216025L3 | Lenti-ORF clone of RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 2 |
CNY 9120.00 |
|
| RC216025L4 | Lenti-ORF clone of RAD17 (mGFP-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 2 |
CNY 9120.00 |
|
| RG216025 | RAD17 (tGFP-tagged) - Human RAD17 homolog (S. pombe) (RAD17), transcript variant 2 |
CNY 7980.00 |
