VPS54 (NM_016516) Human Untagged Clone
CAT＃: SC108738
VPS54 (untagged)-Human vacuolar protein sorting 54 homolog (S. cerevisiae) (VPS54), transcript variant 1
CNY 7160.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L; WR |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108738 sequence for NM_016516 edited (data generated by NextGen Sequencing)
ATGGCTTCAAGCCACAGTTCTTCACCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTT AAAATAGAGGTAGATCCGTCAAAACACATTCGACCTGTGCCATCACTGCCAGATGTGTGT CCCAAGGAACCCACAGGTGATTCACATAGTTTATATGTTGCCCCATCTCTAGTTACAGAT CAACATAGATGGACTGTATATCATTCCAAAGTAAATCTCCCAGCAGCATTAAACGATCCT AGATTAGCAAAAAGAGAATCTGACTTCTTCACAAAAACATGGGGATTGGACTTTGTGGAC ACTGAAGTCATACCTTCATTCTACCTCCCACAGATCAGCAAGGAACATTTTACAGTATAT CAACAGGAAATCTCTCAGAGAGAGAAGATTCATGAGAGATGCAAGAATATTTGTCCTCCT AAAGATACCTTCGAAAGGACTCTTTTACATACTCATGATAAATCCAGGACAGATCTGGAG CAAGTACCTAAGATTTTTATGAAACCAGATTTTGCCTTGGATGATTCCTTAACTTTTAAT TCAGTTTTACCATGGTCTCATTTTAATACTGCTGGTGGAAAAGGAAATCGTGATGCAGCT TCCTCAAAGTTGCTTCAAGAAAAGCTGAGCCATTATCTGGATATTGTGGAAGTAAACATT GCTCACCAGATCTCTCTACGTTCAGAAGCATTTTTTCATGCAATGACCTCTCAACACGAG TTGCAGGACTACCTCAGGAAAACTTCCCAGGCTGTAAAAATGCTTCGAGATAAAATTGCA CAGATTGATAAAGTAATGTGTGAAGGATCACTCCACATTTTAAGACTGGCACTTACCAGA AATAATTGTGTTAAAGTATACAATAAGCTGAAGTTAATGGCCACTGTACACCAGACTCAG CCTACAGTACAGGTGTTATTATCTACTTCTGAATTTGTTGGAGCATTGGACTTAATAGCA ACAACACAAGAGGTTCTACAGCAGGAACTTCAGGGCATTCACAGTTTCCGGCATTTGGGA TCACAGCTTTGTGAATTAGAAAAACTGATAGATAAAATGATGATTGCAGAATTTTCTACT TATTCTCACAGTGACTTAAATAGACCACTGGAAGATGACTGTCAAGTTTTAGAAGAGGAA AGACTAATATCTCTTGTATTTGGACTTTTAAAACAAAGAAAGCTTAATTTTTTAGAAATC TATGGTGAAAAAATGGTTATTACAGCAAAGAATATCATTAAACAGTGTGTGATTAATAAA GTTTCACAAACAGAAGAAATAGACACAGATGTTGTTGTGAAGCTTGCAGATCAGATGAGA ATGTTGAATTTTCCCCAGTGGTTTGATCTGCTCAAGGATATTTTCTCTAAGTTTACAATT TTCCTACAGAGAGTGAAGGCAACATTAAATATCATTCACAGTGTTGTTCTCTCAGTTCTT GACAAAAACCAAAGGACTAGAGAATTGGAAGAGATTTCACAACAGAAGAATGCTGCAAAA GATAATTCACTGGACACAGAGGTGGCTTATTTAATCCATGAAGGCATGTTTATAAGTGAT GCATTCGGTGAGGGTGAGCTAACACCTATAGCAGTTGACACTACCTCTCAAAGAAATGCA TCTCCAAATAGTGAGCCCTGCAGCAGTGATTCTGTATCCGAGCCAGAATGTACTACTGAT TCTTCATCCAGCAAAGAGCACACATCATCATCTGCTATTCCAGGAGGTGTGGATATTATG GTCAGTGAAGATATGAAATTAACTGACTCAGAGCTAGGAAAGCTGGCAAATAATATCCAG GAATTATTATATAGTGCCTCAGATATATGCCATGATCGAGCTGTCAAATTTCTCATGTCA AGAGCAAAGGATGGTTTTCTTGAGAAGCTAAATTCCATGGAATTCATAACACTTTCTAGA TTAATGGAAACATTCATTTTAGACACCGAACAGATCTGTGGAAGAAAAAGCACGTCATTA CTTGGAGCACTTCAGAGCCAAGCTATTAAGTTTGTAAATAGGTTTCATGAAGAGAGAAAA ACCAAGCTCAGCCTCCTCTTAGACAATGAGCGCTGGAAGCAAGCAGATGTTCCTGCAGAA TTTCAGGATCTTGTTGATTCTCTGTCAGATGGGAAGATTGCTTTACCTGAAAAAAAATCA GGAGCCACAGAAGAAAGGAAACCAGCTGAAGTTCTTATTGTCGAGGGACAACAGTATGCA GTTGTTGGAACCGTATTGCTGTTAATAAGAATTATCCTTGAATATTGCCAGTGTGTGGAT AACATCCCATCTGTTACTACTGACATGCTTACTCGTCTGTCAGATTTATTGAAGTACTTC AATTCAAGAAGTTGCCAGTTAGTTCTTGGAGCTGGTGCACTGCAAGTTGTTGGACTAAAA ACGATAACTACAAAAAATTTGGCTCTTTCTTCACGATGTTTGCAGTTAATTGTGCACTAC ATTCCTGTGATCCGGGCTCATTTTGAAGCTCGACTACCACCTAAGCAATATAGCATGCTT AGGCATTTTGATCATATCACTAAGGACTACCATGATCACATAGCTGAAATATCAGCTAAG CTTGTAGCGATAATGGATAGCTTATTTGACAAGCTGTTATCTAAGTATGAAGTGAAGGCT CCTGTTCCTTCTGCCTGTTTCAGGAATATTTGTAAGCAAATGACAAAAATGCACGAAGCT ATATTTGATCTCCTTCCAGAAGAACAAACACAGATGTTATTTTTAAGAATTAATGCAAGT TATAAACTCCACTTGAAAAAGCAGTTATCTCACTTAAATGTGATAAATGATGGAGGACCT CAAAATGGGTTGGTCACAGCAGATGTAGCTTTTTACACTGGAAATCTTCAAGCCTTAAAA GGCCTTAAAGATTTGGACCTAAATATGGCCGAAATTTGGGAGCAGAAGAGGTGA Clone variation with respect to NM_016516.2 >OriGene 5' read for NM_016516 unedited
NGGTTTTCAGGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGGCCACC TCTCACCCCTGAGTGAAGCTGGGCTCGAGAGGGCGGCCCTGTGCTCCCCGGGCCGACTGG CCAGCGGGCGCGGGGCGGGGGCGGGAACCCGGGCTCGGGCCCGGCCGGGCGCCGGGCGGC GGCGGCCGTGGAGCAGCAGCCTCGGTGCGACGTGGAGGGCTGGAGGCGGCGGCGATGCAC TAGGCCTCGCTCAGGGCGGCTGCCCCGGGACCCGCAGTTGAGTGGTGATTTTATGCAATG GCTTCAAGCCACAGTTCTTCACCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTTAAA ATAGAGGTAGATCCGTCAAAACACATTCGACCTGTGCCATCACTGCCAGATGTGTGTCCC AAGGAACCCACAGGTGATTCACATAGTTTATATGTTGCCCCATCTCTAGTTACAGATCAA CATAGATGGACTGTATATCATTCCAAAGTAAATCTCCCAGCAGCATTAAACGATCCTAGA TTAGCAAAAAGAGAATCTGACTTCTTCACAAAAACATGGGGATTGGACTTTGTGGACACT GAAGTCATACCTTCATTCTACCTCCCACAGATCAGCAAGGAACATTTTACAGTATATCAA CAGGAAATCTCTCAGAGAGAGAAGATTCATGAGAGATGCAAGAATATTTGTCCTCCTAAA GATACCTTCGAAGGACTCTTTTCATACTCATGATAAATCCAGGACAGATCTGGAGCCAGT ACCTAAGATTTTATGAAACCAGATTTGCCTCGGATGATCTCTTAACTTTAATNCAGTTTT CCATGGNCTCATTTTATACTGCTGGNGGNAAAGGAATTCGGAGCAGCTCCTTAAGTTGA >OriGene 3' read for NM_016516 unedited
CTTTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTAATTTT TTACAGCTTTATTTTAGACAGATAGTTTAAGAACCAAAGACATACCTCTGTAATGATAAA GGAAAGAAAACAAGCTTTCCTTTTAAGAAACCAAAGAGCACAAAATAAGACTGTTTCATT ATACATAATCACCACAGGATATTAGGCACTCTGACAGGGTTAGGCAAGATTCTTGGTGTG AGGTGAAGCACAGGCACTTTATTTGTACAGTGCTGCTGATTCTAATTTTGAAGGTAGGTA TTATAAAAGTCTTTACTTGTCACCTTATTTCTGGCCCCAACACAGCAGCCTATAGTTTTA AAAGTTCTGTTTCTCCCTGGTCTTTGTTCGTATACACATCGAAAGTAACTTAAAAACAAG GATCCAAGGGGGCCATACTTCATATGTTATCTAAATGTTAATATGAGAACTCAAAAGTAG GCAGATTATATGAATACATATTCTTACCTCTGCTACAAATAAAAACACCCCAAACCCTTC ATCATACTTTTATAAAAATACAGATATTAAACTGTAGCCTTTTATATACCATTTCTGAAA AGTATTTAGTTGCATAAATAGATGCCAGGATCTTTTTTTTTAAGTATTAATTACTTAAAA AAAGGCTTAAGTCTTTCACGTATTTAGAGAGCTCTGGAAGGCTTTACCCAGTTGACATAC CGTGGTAGTAAAAATATTAAATATGGCACACACAAGCCCTGCAACTTGACACTGGCTCAC TGAAACAAACATTGAATTCCTAAATCCCAACCATCCAAAGATGTTTATTTTTTATAGAAA GATCTGTAAACAAAATATAAATTTCTCAACCGCTTTTACTTTCATAGAAAAAACTTCTAC AGAAGGTGACTAACACTTTAAATGTACCATCATTAATAGGGCGGGACACCTTTGAATTTC TTGCCCTGAAAAATGCAAACATTTTAATTTAAATT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016516 |
| Insert Size | 4640 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016516.2, NP_057600.2 |
| RefSeq Size | 3994 bp |
| RefSeq ORF | 2934 bp |
| Locus ID | 51542 |
| UniProt ID | Q9P1Q0 |
| Gene Summary | This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214704 | VPS54 (Myc-DDK-tagged)-Human vacuolar protein sorting 54 homolog (S. cerevisiae) (VPS54), transcript variant 1 |
CNY 7130.00 |
|
| RC214704L3 | Lenti-ORF clone of VPS54 (Myc-DDK-tagged)-Human vacuolar protein sorting 54 homolog (S. cerevisiae) (VPS54), transcript variant 1 |
CNY 9030.00 |
|
| RC214704L4 | Lenti-ORF clone of VPS54 (mGFP-tagged)-Human vacuolar protein sorting 54 homolog (S. cerevisiae) (VPS54), transcript variant 1 |
CNY 9030.00 |
|
| RG214704 | VPS54 (tGFP-tagged) - Human vacuolar protein sorting 54 homolog (S. cerevisiae) (VPS54), transcript variant 1 |
CNY 7890.00 |
