PARP6 (NM_020213) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MGC131971; poly (ADP-ribose) polymerase family, member 6
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_020213, the custom clone sequence may differ by one or more nucleotides ATGTTTACATCCCAACAATGGAAACATCTGAGCAATGATTTCTTGAAGACCCAGCAGGAGAAGAGGCACA GTTGGTTCAAGGCAAGTGGTACCATCAAGAAGTTCCGAGCTGGCCTCAGCATCTTTTCACCCATCCCCAA GTCTCCCAGTTTCCCTATCATACAGGACTCCATGCTGAAAGGCAAACTAGGTGTACCAGAGCTTCGGGTT GGGCGCCTCATGAACCGTTCCATCTCCTGTACCATGAAGAACCCCAAAGTGGAAGTGTTTGGCTACCCTC CCAGCCCCCAGGTCAGTGGTCACTGCAAGAACATTCCCACTCTGGAGTATGGATTCCTCGTTCAGATCAT GAAGTATGCAGAACAGAGGATTCCAACATTGAATGAGTACTGTGTGGTGTGTGATGAGCAGCATGTCTTC CAAAATGGATCTATGCTGAAGCCAGCTGTCTGTACTCGTGAACTATGCGTTTTCTCCTTCTACACACTGG GCGTCATGTCTGGAGCTGCAGAGGAGGTGGCCACTGGAGCAGAGGTGGTGGATCTGCTGGTGGCCATGTG TAGGGCAGCTTTAGAGTCCCCTAGAAAGAGCATCATCTTTGAGCCTTATCCCTCTGTGGTGGACCCCACT GATCCCAAGACTCTGGCCTTTAACCCTAAGAAGAAGAATTATGAGCGGCTTCAGAAAGCTCTGGATAGTG TGATGTCTATTCGGGAGATGACCCAGGGCTGTAGGAATTGCATAAAATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_020213 unedited GGTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAGAGAGAGA GAACTTTAAAGGAAGAAAGAAAATATGAGACAAGAGAGTTTAGGTGTGCTTGGGAACTGT CTTAGGTAATGATCCTGGAAGAGGCCAGCTTGTACTGGAACCCAGATATGCTTAGGAGTC AACCTTGACATTGAAGTCATTTGCATTTCTTTCCTACTGGCTACCAGAGCCTCTCAGTCA TCATACTGAGACTTCAGAAGGCCAAAATTCCCTAGATGTTTTCCTCTGTCCCACTAAGAG CTAGTTTATGGATATGATCATATCAGGAAGAGACTGAGCCTCTCACAAAGGGTGACATGA AAGGTGTAAAGGGATCAGGGCTTCAGTTATTCTATATTTCCCAATCTTTGTGGGAATCTG TTCCTCACCATATCATCCCACGCCTTTCCATGGGATAATAGGGACCTAACAAAGCATGAT ATCCTTATTTCTCACCACTAGGACATCAAAGGCCAGTTCTGGAATGATGACGACTCGGAG GGAGATAATGAATCAGAGGAATTTCTCTATGGCGTTCAGGGGAGCTGTGCAGCTGACCTG TATCGACACCCACAGCTTGATGCAGACATTGAAGCCGTGAAGGAGATCTACAGTGAGAAC TCTGTATCCATCAGAGAATATGGAACTATCGATGACGTGGACATTGACCTCCACATCAAC ATCAGCTTCCTCGATGAGGAAGTCTCTACAGCCTGGGAAGTCCTCCGGACAGAACCTATT GTGTTGAGGCTGCGNATTTCTCTCTCCCAGTACCTAGATGGACCAGAACCATCCATTGAG GGTTTCCAGCCATCAATAAGGAAGGATTTNNGGCTGGTCTTCAGTGAAAAAGACCTGGGT ATGTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_020213 unedited CCGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCCAGTCCCAGCACCTTCC ATTTTATTGTTTTATTTACAAACAGGGTGAAGTCAAAGGGAAAGTCAGGGGATGGAGTCA GTCTGGGCCATCCGAATGTGGAGTAGTTCTTGGGTCAAGCTCTACCATGAAGGCCACCTC TTACATATCCTTTTCCTGCCCCTTGGTAATGGCCCCTTGATTCCTCAGGGCAGCCTCAGC TGCTGTACCTGAACACTTTTATGAGGGCAGATGGATCCTGGGGTAACAGGGGTGGTACGA GGGCTGGGGCCCCCTCAGTTTGTGTAAACCTGAGTTCCGATCACACGCATGATTTCCTTC TGTATCTTGGGGTCCTGAGTATTAATGTTGGCATCGCCCACCTGACCATCCTCATATACA AAGAAGAATCTTGTGCAGACATGGTCGGACACAGGGCACACCCAGATGTTCCCATGCTTC TGGAGGTCCTTAGATGTAATCACTTCACAAAGTGCTATACAGTTTAGATTCCGACTCTGC AGGAACCGTGACTGAATGGATCGGGTCTGGGGGATGGTATTCATCCTGTTGTATCTCTGG ACCAGCTCATCCTTGGAGGGCATCCTGTGCTGTCCTTTTCCCATTCCTGAGTATCCAAAG GAAATACTGGAGATGGGGCTCAGGTAGATGCCTTTGCCATAGGCTGCTCCATGCAGCTGC AGTTTGGTGTAGGATGCATTGACCAGCCCATTGCGCANGATCGAATGCCAGTTCTCAATG TGGGACCCATGGAAGGCAAAGGTGCTGCCATAGAGCTTCTGGGCGGTCCGNAACCGAGCC TCTTGCAGGAGGGCTGCTCANCAGGAGAACTGNTGTAAGGGTGCATGAACTCACCTGCCT GCTGAAAGGTAATTGACATGGGTGAACTGTGCTAAAATGATN
Restriction Sites	NotI-NotI
ACCN	NM_020213
Insert Size	2820 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_020213.1, NP_064598.1
RefSeq Size	2359 bp
RefSeq ORF	750 bp
Locus ID	56965
Gene Summary	Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.[UniProtKB/Swiss-Prot Function]

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