NISCH (NM_007184) Human Untagged Clone
CAT＃: SC108496
NISCH (untagged)-Human nischarin (NISCH)
CNY 10296.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hIRAS; I-1; IR1; IRAS |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_007184 edited
GAATTCGGCACGAGGGTGGCGGCGGAGACCCGAACATGGCGACCGCGCGCACCTTCGGGC CCGAGCGGGAAGCCGAGCCGGCCAAGGAAGCGCGCGTCGTGGGCTCGGAGCTTGTGGACA CTTATACGGTTTACATCATCCAGGTCACTGATGGCAGCCATGAGTGGACAGTAAAGCACC GCTACAGCGACTTCCATGACCTGCATGAAAAGCTCGTTGCAGAGAGAAAGATTGATAAAA ACCTGCTTCCGCCCAAAAAGATAATTGGGAAAAACTCAAGAAGCTTGGTGGAGAAGAGGG AGAAGGATCTGGAGGTCTACCTCCAGAAGCTCCTGGCTGCCTTCCCTGGCGTGACCCCCA GAGTACTGGCCCACTTCTTGCATTTTCACTTCTATGAGATAAATGGCATCACCGCGGCAC TGGCTGAAGAGCTCTTTGAGAAAGGAGAACAGCTCCTGGGGGCCGGCGAGGTCTTTGCCA TTGGACCCCTGCAGCTGTATGCCGTCACGGAGCAGCTGCAGCAGGGAAAGCCCACGTGCG CCAGTGGGGATGCCAAGACCGACCTCGGGCACATCCTGGACTTCACCTGTCGCCTTAAGT ACCTTAAGGTTTCTGGCACAGAAGGACCTTTTGGGACCAGCAACATTCAGGAGCAGCTCC TGCCGTTCGACCTATCAATATTCAAGTCCCTGCATCAGGTGGAGATAAGTCACTGTGATG CTAAGCACATCAGAGGGCTGGTCGCATCGAAGCCCACCTTAGCCACGCTGAGTGTCCGCT TCTCAGCAACCTCGATGAAGGAAGTCCTTGTTCCTGAAGCCTCAGAATTTGATGAGTGGG AGCCTGAAGGCACAACCCTAGAAGGCCCTGTGACTGCCGTCATCCCCACTTGGCAGGCAT TGACCACGCTTGACCTGAGCCACAACAGCATCTCCGAGATCGACGAGTCTGTGAAACTGA TCCCAAAGATTGAGTTCCTGGACCTGAGTCACAATGGATTGCTGGTTGTGGACAATCTGC AGCACCTGTATAACCTTGTGCATCTGGACCTGTCCTACAACAAGCTCTCCTCCTTGGAAG GGCTTCACACCAAGCTGGGGAACATCAAGACCTTAAACCTGGCAGGCAACCTCCTAGAGA GTCTGAGTGGCCTGCACAAGCTCTACTCACTGGTCAACCTGGATCTCCGGGACAACAGGA TCGAACAGATGGAGGAGGTCCGGAGCATAGGCAGCCTCCCGTGTCTGGAGCACGTGTCTC TGCTGAACAACCCTCTGAGCATCATCCCCGACTACCGGACCAAGGTGCTGGCTCAGTTCG GAGAGAGGGCCTCAGAGGTCTGTCTGGATGACACAGTGACCACAGAGAAGGAGCTGGACA CTGTGGAAGTGCTGAAAGCAATTCAGAAAGCCAAGGAGGTCAAGTCCAAACTGAGCAACC CAGAGAAGAAGGGTGGTGAAGACTCCCGGCTCTCAGCTGCCCCCTGCATCAGACCCAGCA GCTCCCCTCCCACTGTGGCTCCCGCATCTGCCTCCCTGCCCCAGCCCATCCTCTCTAACC AAGGAATCATGTTCGTTCAGGAGGAGGCCCTGGCCAGCAGCCTCTCGTCCACTGACAGTC TGACTCCCGAGCACCAGCCCATTGCCCAGGGATGTTCTGATTCCTTGGAGTCCATCCCTG CGGGACAGGCAGCTTCCGATGATTTAAGGGACGTGCCAGGAGCTGTTGGTGGTGCAAGCC CAGAACATGCCGAGCCGGAGGTCCAGGTGGTGCCGGGGTCTGGCCAGATCATCTTCCTGC CCTTCACCTGCATTGGCTACACGGCCACCAATCAGGACTTCATCCAGCGCCTGAGCACAC TGATCCGGCAGGCCATCGAGCGGCAGCTGCCTGCCTGGATCGAGGCTGCCAACCAGCGGG AGGAGGGCCAGGGTGAACAGGGCGAGGAGGAGGATGAGGAGGAGGAAGAAGAGGAGGACG TGGCTGAGAACCGCTACTTTGAAATGGGGCCCCCAGACGTGGAGGAGGAGGAGGGAGGAG GCCAGGGGGAGGAAGAGGAGGAGGAAGAGGAGGATGAAGAGGCCGAGGAGGAGCGCCTGG CTCTGGAATGGGCCCTGGGCGCGGACGAGGACTTCCTGCTGGAGCACATCCGCATCCTCA AGGTGCTGTGGTGCTTCCTGATCCATGTGCAGGGCAGTATCCGCCAGTTCGCCGCCTGCC TTGTGCTCACCGACTTCGGCATCGCAGTCTTCGAGATCCCGCACCAGGAGTCTCGGGGCA GCAGCCAGCACATCCTCTCCTCCCTGCGCTTTGTCTTTTGCTTCCCGCATGGCGACCTCA CCGAGTTTGGCTTCCTCATGCCGGAGCTGTGTCTGGTGCTCAAGGTACGGCACAGTGAGA ACACGCTCTTCATTATCTCGGACGCCGCCAACCTGCACGAGTTCCACGCGGACCTGCGCT CATGCTTTGCACCCCAGCACATGGCCATGCTGTGTAGCCCCATCCTCTACGGCAGCCACA CCAGCCTGCAGGAGTTCCTGCGCCAGCTGCTCACCTTCTACAAGGTGGCTGGCGGCTGCC AGGAGCGCAGCCAGGGCTGCTTCCCCGTCTACCTGGTCTACAGTGACAAGCGCATGGTGC AGACGGCCGCCGGGGACTACTCAGGCAACATCGAGTGGGCCAGCTGCACACTCTGTTCAG CCGTGCGGCGCTCCTGCTGCGCGCCCTCTGAGGCCGTCAAGTCCGCCGCCATCCCCTACT GGCTGTTGCTCACGCCCCAGCACCTCAACGTCATCAAGGCCGACTTCAACCCCATGCCCA ACCGTGGCACCCACAACTGTCGCAACCGCAACAGCTTCAAGCTCAGCCGTGTGCCGCTCT CCACCGTGCTGCTGGACCCCACACGCAGCTGTACCCAGCCTCGGGGCGCCTTTGCTGATG GCCACGTGCTAGAGCTGCTCGTGGGGTACCGCTTTGTCACTGCCATCTTCGTGCTGCCCC ACGAGAAGTTCCACTTCCTGCGCGTCTACAACCAGCTGCGGGCCTCGCTGCAGGACCTGA AGACTGTGGTCATCGCCAAGACCCCCGGGACGGGAGGCAGCCCCCAGGGCTCCTTTGCGG ATGGCCAGCCTGCCGAGCGCAGGGCCAGCAATGACCAGCGTCCCCAGGAGGTCCCAGCAG AGGCTCTGGCCCCGGCCCCAGTGGAAGTCCCAGCTCCAGCCCCTGCAGCAGCCTCAGCCT CAGGCCCAGCGAAGACTCCGGCCCCAGCAGAGGCCTCAACTTCAGCTTTGGTCCCAGAGG AGACGCCAGTGGAAGCTCCAGCCCCACCCCCAGCCGAGGCCCCTGCCCAGTACCCGAGTG AGCACCTCATCCAGGCCACCTCGGAGGAGAATCAGATCCCCTCGCACTTGCCTGCCTGCC CGTCGCTCCGGCACGTCGCCAGCCTGCGGGGCAGCGCCATCATCGAGCTCTTCCACAGCA GCATTGCTGAGGTTGAAAACGAGGAGCTGAGGCACCTCATGTGGTCCTCGGTGGTGTTCT ACCAGACCCCAGGGCTGGAGGTGACTGCCTGCGTGCTGCTCTCCACCAAGGCTGTGTACT TTGTGCTCCACGACGGCCTCCGCCGCTACTTCTCAGAGCCACTGCAGGATTTCTGGCATC AGAAAAACACCGACTACAACAACAGCCCTTTCCACATCTCCCAGTGCTTCGTGCTAAAGC TTAGTGACCTGCAGTCAGTCAATGTGGGGCTTTTCGACCAGCATTTCCGGCTGACGGGTT CCACCCCGATGCAGGTGGTCACGTGCTTGACGCGGGACAGCTACCTGACGCACTGCTTCC TCCAGCACCTCATGGTCGTGCTGTCCTCTCTGGAACGCACGCCCTCGCCGGAGCCTGTTG ACAAGGACTTCTACTCCGAGTTTGGGAACAAGACCACAGGGAAGATGGAGAACTACGAGC TGATCCACTCTAGTCGCGTCAAGTTTACCTACCCCAGTGAGGAGGAGATTGGGGACCTGA CGTTCACTGTGGCCCAAAAGATGGCTGAGCCAGAGAAGGCCCCAGCCCTCAGCATCCTGC TGTACGTGCAGGCCTTCCAGGTGGGCATGCCACCCCCTGGGTGCTGCAGGGGCCCCCTGC GCCCCAAGACACTCCTGCTCACCAGCTCCGAGATCTTCCTCCTGGATGAGGACTGTGTCC ACTACCCACTGCCCGAGTTTGCCAAAGAGCCGCCGCAGAGAGACAGGTACCGGCTGGACG ATGGCCGCCGCGTCCGGGACCTGGACCGAGTGCTCATGGGCTACCAGACCTACCCGCAGG CCCTCACCCTCGTCTTCGATGACGTGCAAGGTCATGACCTCATGGGCAGTGTCACCCTGG ACCACTTTGGGGAGGTGCCAGGTGGCCCGGCTAGAGCCAGCCAGGGCCGTGAAGTCCAGT GGCAGGTGTTTGTCCCCAGTGCTGAGAGCAGAGAGAAGCTCATCTCGCTGTTGGCTCGCC AGTGGGAGGCCCTGTGTGGCCGTGAGCTGCCTGTCGAGCTCACCGGCTAGCCCAGGCCAC AGCCAGCCTGTCGTGTCCAGCCTGACGCCTACTGGGGCAGGGCAGCAGGCTTTTGTGTTC TCTAAAAATGTTTTATCCTCCCTTTGGTACCTTAATTTGACTGTCCTCGCAGAGAATGTG AACATGTGTGTGTGTTGTGTTAATTCTTTCTCATGTTGGGAGTGAGAATGCCGGGCCCCT CAGGGCTGTCGGTGTGCTGTCAGCCTCCCACAGGTGGTACAGCCGTGCACACCAGTGTCG TGTCTGCTGTTGTGGGACCGTTGTTAACACGTGACACTGTGGGTCTGACTTTCTCTTCTA CACGTCCTTTCCTGAAGTGTCGAGTCCAGTCCTTTGTTGCTGTTGCTGTTGCTGTTGCTG TTGCTGTTGGCATCTTGCTGCTAATCCTGAGGCTGGTAGCAGAATGCACATTGGAAGCTC CCACCCCATATTGTTCTTCAAAGTGGAGGTCTCCCCTGATCCAGACAAGTGGGAGAGCCC GTGGGGGCAGGGGACCTGGAGCTGCCAGCACCAAGCGTGATTCCTGCTGCCTGTATTTTT TATTCCAATAAAGCAGAGTTTGACACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAACTCGAC >OriGene 5' read for NM_007184 unedited
AATTTTCCCGCCCGTTGCCGCTTTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC GAATTCGGCACGAGGGTGGCGGCGGAGACCCGAACATGGCGACCGCGCGCACCTTCGGGC CCGAGCGGGAAGCCGAGCCGGCCAAGGAAGCGCGCGTCGTGGGCTCGGAGCTTGTGGACA CTTATACGGTTTACATCATCCAGGTCACTGATGGCAGCCATGAGTGGACAGTAAAGCACC GCTACAGCGACTTCCATGACCTGCATGAAAAGCTCGTTGCAGAGAGAAAGATTGATAAAA ACCTGCTTCCGCCCAAAAAGATAATTGGGAAAAACTCAAGAAGCTTGGTGGAGAAGAGGG AGAAGGATCTGGAGGTCTACCTCCAGAAGCTCCTGGCTGCCTTCCCTGGCGTGACCCCCA GAGTACTGGCCCACTTCTTGCATTTTCACTTCTATGAGATAAATGGCATCACCGCGGCAC TGGCTGAAGAGCTCTTTGAGAAAGGAGAACAGCTCCTGGGGGCCGGCGAGGTCTTTGCCA TTGGACCCCTGCAGCTGTATGCCGTCACGGAGCAGCTGCAGCAGGGAAAGCCCACGTGCG CCAGTGNGGATGCCAAGACCGACCTCGGGCACATCCTGGACTTCACCTGTCGCCTTTAGT ACCTTAAGGTTTCTGGCACAGAAGGACCTTTTGGNGACCAGCACATTCAGGAGCAGCTCC TGCCGTTCGACCTATCATATTCAAGTCCCTGCATCAGGTGGNAGATAGTCACTGTGATGC TAAGCACATCAGAGGGCTGGTCGCATCGAAGCCACCTTAGCACGCTGAGTGTCCCGCTCT CAGCACCTCGATGAGGAAGTCCNTGNTNNCTGAGCC >OriGene 3' read for NM_007184 unedited
NGGGGGGTACCACTATGNNACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTCGGGGTCAAACTCTGCTTTATTGGAATAAAAAATACAG GCAGCAGGAATCACGCTTGGTGCTGGCAGCTCCAGGTCCCCTGCCCCCACGGGCTCTCCC ACTTGTCTGGATCAGGGGAAACCTCCACTTTGAAAAACAATATGGGGTGGGAGCTTCCAA TGTGCATTCTGCTACCAGCCTCAGGATTAGCAGCAAGATGCCAACAGCAACAGCAACAGC AACAGCAACAGCAACAAAGGACTGGACTCGACACTTCAGGAAAGGACGTGTAAAAAAAAA AGTCAGACCCCCAGTGTCACGTGTTAACAACGGTCCCACAACAGCAAACACGAAACTGGT GTGCACGGCTGTACCACCTGGGGGAGGCTGAAAGCACACCGACAGCCCTGAGGGGCCCGG CATTCTTACTCCCCACATGAGAAAGAATTAACACAACCCCCCCCCCTGTTCACATTTTCT GCGAGGACAGTCAAATTAAGGTACCCAAGGGGGGATAAAACATTTTTAGAGAAAAAAAAA ACCTGGTGCCCTGCCCCGAAGGCCTTAGGCTGGACACCAAAGGCTGGCTTTGGCCCGGGC TAACCCGGGAAACCTGAAAGGGAGTTCACGGGCCCCCCGGGGCCCCCCCCTGGGGAAGCC CACCAGGAGAAAGAAGCTTTTCTTTGGCTCTTAAAACCTGGGGACAAAAACCTTGCCACT GGGGATTAAACGGCCCTGGGGGTGCTTTTACCCGGGGCACCATGGGCCTCTCCCCAAAAG GGGCCAGGTGAAAACTCGCCCCGAAAGACAATAACCTTCCCTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_007184 |
| Insert Size | 5500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007184.2, NP_009115.2 |
| RefSeq Size | 5139 bp |
| RefSeq ORF | 4515 bp |
| Locus ID | 11188 |
| UniProt ID | Q9Y2I1 |
| Domains | LRR, PX, LRR_SD22 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as IRAS-M). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206026 | NISCH (Myc-DDK-tagged)-Human nischarin (NISCH) |
CNY 10288.00 |
|
| RC206026L1 | Lenti ORF clone of Human nischarin (NISCH), Myc-DDK-tagged |
CNY 12688.00 |
|
| RC206026L2 | Lenti ORF clone of Human nischarin (NISCH), mGFP tagged |
CNY 14730.00 |
|
| RC206026L3 | Lenti ORF clone of Human nischarin (NISCH), Myc-DDK-tagged |
CNY 14730.00 |
|
| RC206026L4 | Lenti ORF clone of Human nischarin (NISCH), mGFP tagged |
CNY 14730.00 |
|
| RG206026 | NISCH (tGFP-tagged) - Human nischarin (NISCH) |
CNY 11888.00 |
