NNT (NM_012343) Human Untagged Clone
CAT＃: SC108450
NNT (untagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 7568.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GCCD4 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC108450 representing NM_012343.
Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTCGTGTCCTCTACTTAGCAATTTGGGGTCCTGT AAGGGTCTACGTGTGAAGAAGGATTTTTTACGAACATTTTATACTCACCAAGAACTGTGGTGTAAAGCG CCTGTAAAACCAGGAATTCCATATAAGCAACTGACTGTTGGAGTCCCCAAAGAGATATTCCAAAATGAG AAGCGAGTGGCATTGTCTCCTGCTGGTGTTCAGAACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAA TCGGGTGCGGGCGAAGCTTCCAAGTTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAATCCAAGGG GCAAAGGAAGTGCTGGCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGTTAATCCAACATTAGGT GTTCATGAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAGTTTTATTTACCCAGCCCAAAATCCA GAGTTGCTAAATAAACTTTCCCAAAGAAAAACTACAGTTCTGGCAATGGACCAGGTTCCAAGAGTCACA ATTGCTCAGGGATATGATGCGCTAAGCTCCATGGCCAACATTGCGGGTTATAAGGCTGTTGTCCTAGCA GCAAATCATTTTGGACGTTTTTTTACTGGTCAGATCACAGCTGCTGGAAAAGTTCCTCCAGCTAAGATT CTGATAGTTGGTGGTGGTGTTGCTGGGCTTGCTTCTGCAGGCGCAGCAAAGTCGATGGGTGCAATTGTT CGAGGATTTGACACAAGAGCTGCAGCTTTGGAACAGTTCAAGTCTCTTGGTGCTGAGCCCTTGGAGGTG GACTTGAAGGAATCTGGTGAGGGACAAGGAGGATATGCAAAAGAGATGTCCAAAGAGTTCATTGAAGCT GAAATGAAACTCTTTGCTCAACAATGCAAGGAGGTAGACATCCTTATCAGCACAGCACTTATTCCAGGT AAAAAAGCTCCAGTTTTATTTAATAAAGAAATGATTGAGTCAATGAAGGAAGGTTCAGTTGTTGTGGAT TTAGCTGCTGAGGCTGGTGGAAACTTTGAAACCACTAAGCCAGGAGAACTCTACATTCATAAGGGAATT ACTCACATAGGCTACACAGACCTGCCCAGCCGAATGGCCACTCAGGCCAGCACCCTATATTCCAACAAC ATCACCAAACTCCTGAAGGCCATCAGCCCGGACAAAGATAATTTTTATTTTGATGTGAAAGATGACTTT GACTTTGGTACGATGGGTCATGTCATTAGAGGAACTGTAGTGATGAAAGATGGTAAAGTGAATTTCCCA GCTCCCACACCGAAAAATATTCCTCAAGGTGCCCCAGTAAAACAGAAGACAGTGGCTGAGCTGGAAGCT GAAAAAGCAGCTACCATTACACCCTTCAGGAAGACAATGTCAACGGCTTCTGCATATACAGCAGGTCTC ACAGGGATACTGGGTTTGGGCATTGCGGCTCCCAATCTAGCCTTTTCTCAGATGGTGACCACTTTTGGC TTGGCTGGCATTGTGGGGTATCATACCGTCTGGGGAGTGACCCCTGCTCTCCACTCACCACTGATGTCT GTGACAAATGCAATCTCAGGGCTGACTGCAGTTGGTGGGTTGGCACTGATGGGAGGACATTTGTATCCT TCCACAACTTCTCAGGGCCTTGCTGCTCTTGCTGCATTCATATCCTCTGTCAACATTGCAGGTGGCTTT CTGGTGACTCAGAGAATGCTGGACATGTTCAAGCGTCCCACTGACCCCCCAGAATACAACTACCTGTAC CTGCTCCCTGCCGGCACCTTTGTTGGTGGATATTTAGCTGCCCTCTACAGTGGTTATAACATTGAACAG ATCATGTACCTAGGCTCGGGTTTGTGCTGTGTCGGTGCCTTGGCTGGCCTCTCCACCCAGGGAACAGCA CGTCTTGGCAATGCACTGGGCATGATTGGGGTTGCTGGAGGACTGGCAGCCACCCTCGGAGTCCTAAAA CCGGGCCCAGAATTACTAGCTCAGATGTCTGGAGCGATGGCTTTGGGTGGTACCATTGGATTGACAATT GCCAAACGCATCCAGATTTCTGATTTACCTCAATTAGTTGCTGCTTTTCACAGTTTAGTGGGTTTGGCA GCTGTACTTACTTGCATAGCTGAGTACATTATAGAATATCCACATTTTGCTACGGATGCAGCAGCAAAT CTCACCAAGATTGTGGCCTACCTCGGCACTTACATTGGTGGCGTCACCTTTAGTGGGTCTCTCATTGCC TATGGAAAATTGCAGGGTCTCCTGAAATCTGCCCCTCTCCTACTGCCTGGAAGGCACTTACTCAATGCA GGCTTACTGGCTGCTAGTGTGGGCGGGATAATCCCATTCATGGTGGACCCAAGCTTTACTACTGGCATC ACCTGTCTGGGTTCAGTGTCTGCTCTCTCTGCTGTCATGGGTGTGACTTTGACAGCTGCTATTGGGGGT GCTGACATGCCCGTCGTTATCACTGTGCTGAACAGCTACTCAGGCTGGGCCCTGTGTGCAGAGGGCTTC CTGCTCAACAACAATCTGCTGACCATCGTGGGTGCACTCATAGGCTCGTCTGGTGCTATCCTGTCATAC ATCATGTGTGTGGCAATGAATCGCTCCCTGGCTAATGTGATTCTTGGAGGCTATGGCACCACTTCAACA GCTGGTGGAAAACCCATGGAAATTTCTGGCACACATACGGAAATCAACCTTGACAATGCAATTGACATG ATTCGAGAAGCTAATAGCATTATTATTACACCAGGCTATGGTCTCTGTGCAGCCAAAGCTCAATACCCC ATTGCTGATTTGGTAAAGATGCTCACTGAGCAAGGCAAAAAAGTCAGGTTTGGAATTCACCCAGTTGCA GGCCGAATGCCTGGTCAGCTTAATGTGCTGCTGGCTGAGGCTGGTGTGCCATATGACATTGTGTTGGAA ATGGATGAGATCAACCATGATTTTCCAGATACTGATTTGGTCCTTGTAATTGGAGCTAATGACACTGTT AATTCAGCAGCTCAAGAAGATCCCAACTCTATTATTGCAGGCATGCCAGTCCTTGAGGTCTGGAAATCA AAGCAGGTGATTGTTATGAAGAGGTCTTTGGGTGTTGGCTATGCTGCAGTGGACAATCCAATCTTCTAC AAACCTAACACGGCCATGCTTCTAGGTGATGCCAAGAAAACATGTGACGCGCTCCAGGCGAAAGTTAGA GAATCCTATCAGAAGTAA >OriGene 5' read for NM_012343 unedited
GGTTTTAGGATTTGTNATCCGACTTCATATAGGGCGGCCGCGCAATTCGCACGAGGGGCA CAGCCCAGGCTGTCAGCCTCCCGGCCCAGTGATTTGCCTTCAAGGAAACTGGGGAGTCAG AAAATTGGGAACTCATATCAACATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTC GTGTCCTCTACTTAGCAATTTGGGGTCCTGTAAGGGTCTACGTGTGAAGAAGGATTTTTT ACGAACATTTTATACTCACCAAGAACTGTGGTGTAAAGCGCCTGTAAAACCAGGAATTCC ATATAAGCAACTGACTGTTGGAGTCCCCAAAGAGATATTCCAAAATGAGAAGCGAGTGGC ATTGTCTCCTGCTGGTGTTCAGAACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATC GGGTGCGGGCGAAGCTTCCAAGTTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAAT CCAAGGGGCAAAGGAAGTGCTGGCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGT TAATCCAACATTAGGTGTTCATGAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAG TTTTATTTACCCAGCCCAAAATCCAGAGTTGCTAAATAAACTTTCCCAAAGAAAAACTAC AGTTCTGGCAATGGACCAGGTTCCAAGAGTCACAATTGCTCAGGGATATGATGCGCTAAG CTCCATGGCCAACATTGCGGGTTATAAAGCTGTTGTCCTAGCAGCANATCATTTTGGACG TTTTTTTACTGGTCAGATCACAGCTGCTGGGAAAGTTCCTCCAGCTAAGATCTGAAGTTG GTGGTGGGTGTTGCTGGGCTTGCTTCTGCAGCGCAGCANATCGAGGGTGCAATTGTCGAG GATTGACACAGACTGCAGCTTGGAACAGTTC >OriGene 3' read for NM_012343 unedited
CCTTCGTTCTATTTCCCCACCTTCCACACCAGGNTTTGTGTGNANGAAAACCGCAATCCG AGGCCNGGTTTTTTTCCTTTTTTTTTTTTTGCCAACTGTTTTACTCTTTTATAGCCAAAG TTGAATTCCACTCAAGTGCACTTAATATAGTACTACACATTATAAGTTGATCGAAATTAA AGAGATCTCCAATTAGAAATGGGATCTTCAAAAATACTATTACTAATTTTCAAGCTGGAT GTTTCATTTTTTTTCTAATGAAACATATAAAGCAAATGATTTGTTCCCAGCCTCTCTGAA AGATTATTTAGCCCTTTAATGTATTAAAATAGTAGTGAGAAAATGTGCCCTTGAAATATA GACTTTGAAGTACAATTTAAAAAATTAAAACTGACTTGATATGATTAATTCAAACTGCGG TTGAATTGCAAGGGGACAGCTCATAAGCTCATAAAAGGTTCAAATTAGCCACATTTGTTG AAAAAAAAAACATAAAATGCAGGTGCCTGCAATGGTTACATTGTGTCAGATATTTCAGAA GCATGTATAACGCTTGCCTTGAAATCCTAAACACTTACCTTTTTTAAACATTTTTTGGCT ACTGAGGAGGTATCAAAAATTCAACAGGACTACCTCTAACATGACGGCTGTTCTTTCTCC CTTTATTCAACACCTTTTGACTTCCTACACAAAAAAAATATGGCCTAGAAAGACATTTAG AATCTTTCCATTCCTTTTTAAAAATTGAGGGATTACCGTATTTTGAAAATTTTTTTCTAT ACCGCTCTTTGCCTTTGCTTTTTTCCACCCTTTATTTCCTTCCACAAGTTCTCTTTCCCA ATGGTCATTCCCCTTGTTTACGGGACCTCTCCTCGCGTTGTTCCCAAACATTGCAAGGTG GGTCTCTTAACTTAACGCTGGGTCCTCTATAATAAATCCCGGGAGGAATTTGTTAATCCC CGCCCCGAAAGGGTAAAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012343 |
| Insert Size | 3261 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012343.2 |
| RefSeq Size | 4600 bp |
| RefSeq ORF | 3261 bp |
| Locus ID | 23530 |
| UniProt ID | Q13423 |
| Domains | PNTB, AlaDh_PNT_C, AlaDh_PNT_N |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism |
| MW | 113.9 kDa |
| Gene Summary | This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same isoform. |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224002 | NNT (Myc-DDK-tagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 7560.00 |
|
| RC224002L1 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 9960.00 |
|
| RC224002L2 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 10260.00 |
|
| RC224002L3 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 10260.00 |
|
| RC224002L4 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 10260.00 |
|
| RG224002 | NNT (tGFP-tagged) - Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 9160.00 |
