ZNF383 (NM_152604) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSD17; Zfp383
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC108107 sequence for NM_152604 edited (data generated by NextGen Sequencing) ATGGCTGAGGGATCAGTGATGTTCAGTGATGTGTCCATAGACTTCTCTCAGGAGGAGTGG GACTGCCTGGACCCTGTTCAGAGGGACTTATACAGAGATGTGATGTTGGAGAACTACGGC AATCTGGTTTCAATGGGACTTTACACTCCTAAGCCTCAAGTGATCTCCTTATTGGAACAA GGGAAAGAGCCCTGGATGGTTGGCAGAGAGCTTACAAGAGGCCTGTGTTCAGATCTGGAA TCGATGTGTGAAACCAAGTTATTATCTCTAAAGAAGGAAGTTTATGAAATAGAATTATGC CAGAGGGAGATAATGGGACTTACAAAGCATGGCCTTGAGTACTCCAGTTTTGGAGATGTT TTGGAATATAGAAGCCACCTTGCAAAACAACTGGGATATCCAAATGGGCATTTTAGTCAA GAAATATTCACTCCTGAATACATGCCCACATTTATTCAACAGACATTCCTTACTCTCCAT CAAATAATTAATAATGAAGACAGACCCTATGAATGTAAGAAATGTGGAAAGGCCTTTAGT CAGAACTCACAATTTATTCAACATCAGAGAATTCATATTGGTGAAAAATCTTATGAATGT AAAGAGTGTGGGAAATTCTTTAGTTGTGGTTCACATGTTACTCGGCATCTGAAAATTCAT ACTGGCGAAAAACCCTTTGAATGTAAGGAATGTGGAAAGGCCTTCAGTTGTAGCTCATAC CTTTCTCAACATCAGAGAATCCATACCGGTAAGAAACCCTATGAATGTAAGGAATGTGGG AAGGCCTTTAGTTATTGCTCAAATCTTATTGACCATCAGCGAATTCACACTGGTGAAAAA CCTTATGAATGTAAAGTATGTGGGAAAGCCTTTACTAAGAGCTCACAACTTTTTCAGCAT GCACGAATTCATACAGGTGAGAAACCCTATGAATGTAAGGAATGTGGCAAAGCCTTTACC CAGAGCTCAAAGCTTGTTCAACATCAGAGAATTCATACTGGTGAGAAACCCTATGAGTGC AAGGAATGTGGCAAAGCCTTTAGTAGTGGCTCAGCACTTACTAATCATCAGAGAATTCAC ACTGGTGAGAAACCCTATGATTGTAAGGAATGTGGAAAGGCTTTTACTCAGAGCTCACAG CTTCGTCAACATCAGAGAATTCACGCTGGTGAGAAACCCTTTGAATGTCTTGAATGTGGG AAGGCCTTTACTCAGAACTCACAACTTTTCCAGCATCAGAGAATTCATACAGATGAAAAA CCATATGAATGTAATGAATGTGGAAAGGCCTTTAATAAATGCTCAAACCTTACTCGACAT CTGAGAATTCACACTGGTGAAAAGCCCTATAACTGTAAGGAATGTGGGAAGGCTTTTAGT AGTGGCTCGGATCTCATTCGTCATCAGGGAATTCATACTAATAAATAA Clone variation with respect to NM_152604.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_152604 unedited TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCGGTGAGCGGCCTGG GTCTTGCAGGCTCGGCTGGGGCCCGCAGGAGAACGGCCTCAAGGAAGACTAACCATCTGC AATACTAGAAGCCATGGCTGAGGGATCAGTGATGTTCAGTGATGTGTCCATAGACTTCTC TCAGGAGGAGTGGGACTGCCTGGACCCTGTTCAGAGGGACTTATACAGAGATGTGATGTT GGAGAACTACGGCAATCTGGTTTCAATGGGACTTTACACTCCTAAGCCTCAAGTGATCTC CTTATTGGAACAAGGGAAAGAGCCCTGGATGGTTGGCAGAGAGCTTACAAGAGGCCTGTG TTCAGATCTGGAATCGATGTGTGAAACCAAGTTATTATCTCTAAAGAAGGAAGTTTATGA AATAGAATTATGCCAGAGGGAGATAATGGGACTTACAAAGCATGGCCTTGAGTACTCCAG TTTTGGAGATGTTTTGGAATATAGAAGCCACCTTGCAAAACAACTGGGATATCCAAATGG GCATTNTAGTCAAGAAATATTCACTCCTGAATACATGCCCACATTTATTCAACAGACATT CCTTACTCTCCATCANATAATTAATAATGAAGACAGACCCTATGAATGTAAGAAATGTGG AAAGGGCCTTTAGTCAGAACTCACAATTTATTNCACATCAGAGAATTCATATTGGTGAAA AATCTTATGAAATGTAAGAGTGTGGGAAATTCTTTANTTGTGGNTCACATGNNTACTCGC ATCTGAAANTCATACTGGCGANNNACCCTTTGATGTANNNGATGTGNAAGCCCTCAGTGT AGCTATACCTTCTCACATCAGAGATCCTACCCGTAGAACCTATGATGTAGGGATGTGGAG GCCTTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_152604 unedited CGTTGAACGCGCCCGCTATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATGTCTGAAAAT AATAAAAATCTATATTTACTGAAATGGCACTCATGAAATCTTTGGCATGATAAAAGCAGT TAAGGGTACAGCATGCATATCATAATTACACTTTCTTAAAAAAGCAAAGTATAGCACGAT TTTCTCTACCAAATTGAGAGCAGCCATCTATAAGTATGATTACAATTAATTTTGGCTTTT TAGTTTGTAATTTTTTGCTTTGTGATTTTGGCTTAACAATTAAAAATGTACATTTGTAAA ATAAAAAGACCGGCCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTCGGAGGCCGAGGC GGGTGGATCACCTGAAGTCGGGAGATTGAGACCATCCTGGCTAACATGGTGAAACCCCGT CTCTACTAAAAATACAAAAAATTAGCCGGGTGTTGTGGCACATGCCTGTAATCCCAACTA CTCAAAAGGCTGAGGCAGAAGAACCACTTGAACCTGGGAGGCGAAGGTTGTGACGAGCCG AGATTGCACCATTGCACTCCAGCCTAGGCAACAAGAGCGAAACTCCGTCTCAACAAANAA TAAAGCCAGGTGCAATCGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGT GGATCACCTGAGGTCAGGAGTTCAAGACTATCCTGACCAACATGGAGAAACCCTGTCTCT ACTAAAATAGAAAAATAGCAGGGTGTGGTGGCACACGCCTGTGATCCCAGCTACTCAGGA GTCTGAAGCAGGAGAATTGCCTTGAACCCGCGAGGTGGAGTTGGANCAGGCCGAGATTGC CCCCTGCCTCCAACCGGGCAAAAAATAAAATTCGCCTCNAACCTTTTATATTTGATTAAA TAACCAAAGGCTACCCTACCTCCAAAACAAGTTAACCTCCCAG
Restriction Sites	NotI-NotI
ACCN	NM_152604
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_152604.1, NP_689817.1
RefSeq Size	1694 bp
RefSeq ORF	1428 bp
Locus ID	163087
UniProt ID	Q8NA42
Domains	KRAB, zf-C2H2
Protein Families	Transcription Factors
Gene Summary	The protein encoded by this gene is a KRAB-related zinc finger protein that inhibits the transcription of some MAPK signaling pathway genes. The repressor activity resides in the KRAB domain of the encoded protein. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. All four variants encode the same protein.

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