PDPK1 (NM_031268) Human Untagged Clone
CAT＃: SC107892
PDPK1 (untagged)-Human 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 2
CNY 7220.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PDK1; PDPK2; PDPK2P; PRO0461 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_031268, the custom clone sequence may differ by one or more nucleotides
ATGGCCAGGACCACCAGCCAGCTGTATGACGCCGTGCCCATCCAGTCCAGCGTGGTGTTATGTTCCTGCC CATCCCCATCAATGGTGAGGACCCAGACTGAGTCCAGCACGCCCCCTGGCATTCCTGGTGGCAGCAGGCA GGGCCCCGCCATGGACGGCACTGCAGCCGAGCCTCGGCCCGGCGCCGGCTCCCTGCAGCATGCCCAGCCT CCGCCGCAGCCTCGGAAGAAGCGGCCTGAGGACTTCAAGTTTGGGAAAATCCTTGGGGAAGGCTCTTTTT CCACGGTTGTCCTGGCTCGAGAACTGGCAACCTCCAGAGAATATGCGACCAGGGCCAACTCATTCGTGGG AACAGCGCAGTACGTTTCTCCAGAGCTGCTCACGGAGAAGTCCGCCTGTAAGAGTTCAGACCTTTGGGCT CTTGGATGCATAATATACCAGCTTGTGGCAGGACTCCCACCATTCCGAGCTGGAAACGAGTATCTTATAT TTCAGAAGATCATTAAGTTGGAATATGACTTTCCAGAAAAATTCTTCCCTAAGGCAAGAGACCTCGTGGA GAAACTTTTGGTTTTAGATGCCACAAAGCGGTTAGGCTGTGAGGAAATGGAAGGATACGGACCTCTTAAA GCACACCCGTTCTTCGAGTCCGTCACGTGGGAGAACCTGCACCAGCAGACGCCTCCGAAGCTCACCGCTT ACCTGCCGGCTATGTCGGAAGACGACGAGGACTGCTATGGCAATTATGACAATCTCCTGAGCCAGTTTGG CTGCATGCAGGTGTCTTCGTCCTCCTCCTCACACTCCCTGTCAGCCTCCGACACGGGCCTGCCCCAGAGG TCAGGCAGCAACATAGAGCAGTACATTCACGATCTGGACTCGAACTCCTTTGAACTGGACTTACAGTTTT CCGAAGATGAGAAGAGGTTGTTGTTGGAGAAGCAGGCTGGCGGAAACCCTTGGCACCAGTTTGTAGAAAA TAATTTAATACTAAAGATGGGCCCAGTGGATAAGCGGAAGGGTTTATTTGCAAGACGACGACAGCTGTTG CTCACAGAAGGACCACATTTATATTATGTGGATCCTGTCAACAAAGTTCTGAAAGGTGAAATTCCTTGGT CACAAGAACTTCGACCAGAGGCCAAGAATTTTAAAACTTTCTTTGTCCACACGCCTAACAGGACGTATTA TCTGATGGACCCCAGCGGGAACGCACACAAGTGGTGCAGGAAGATCCAGGAGGTTTGGAGGCAGCGATAC CAGAGCCACCCGGACGCCGCTGTGCAGTGA >OriGene 5' read for NM_031268 unedited
CAAATTTTGTATACGAACTCACTATAGGGCGGCCGCGACTTCGGCACGAGGCACAGGAGG GCGAGAACAGCACACTTCAACCCCAGCTTGCTGGTCGGCTTTCCTCTAGAGAGAGCCGGT TTTGGGGCCATTTCCCTTTGATGCTTTGGTGGCCTTGCCCCGCTCTGCAGCACAGACAGG CCAGATGCATTTGTCCTTTGCCTAGCTACTCCCCAGGTAGAGAGTGCTCCTGGTGGCCTG GCAGGTCTGGGCCCTTCTCTCCCTGCCCAGGTTGTCCCTGGAGGGCAGCCCTCACTCCCT TTNGGGGGAGAGGCAGACATTGCTGCCCACAGACCTGCCTCTGACTCAACTGTGTCCACC CTCCCTGGTCCCTACCCCCAAGTCACAGGTGACTCAGCAGTGACCCTGTGTGCCAGGCCA GATCCAAACTGAGAGGGAAGGTGTCGTTTTTACACTGCTAATGACGAGAGTGGCTCTTTT TAGCTAGGCGAGTACAGACGGGGCCTGGGAGGGGGCAGAGATGTTCCCCAGGCCCTGCCT GTGGTTCCTGCCTGGGCCTTGGCTGCTGCTGTGTGAGAGCTGCATGTGAGCCTGTGACCG TGAGCTGGGGTGAGCTGGGCCGCACCTCCCCTGGGGCCCCAGGGAGCAGGACGCTTCCGG GGCCCAGCACGTTGCCCTGGGCCTGTGGCCGGAGTCGGAGTCCTCTCTCCTCCTCCTGGC TTTTGGAAGGCTTGGCTGTGTGGGGGAGTCTCTCTTACCCTTTCAGGATTTCTGGTCAGG CTTCCTNCTCTCANAGCTATTTACCCATTCAGACGTNCTGTGCTCCTGTAGGAGAGTGCT CTCTCGATCTCCAGGGCGTCTGTTATAAGAAACAAGTGACAAGGCGTGGCTTTATTGAGC CTCGCTGGCTGCTGGGGGACTCTTCCGGCGTCTTCTTTGAAGAACACACTTCTGGCGTAC TGA >OriGene 3' read for NM_031268 unedited
CGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTACACTCTACATGCT TTTTATTACAAGACTACTGACCATACGAGGAAAATTCATCATCTGGTAACTACCCCTAAA GCACAAGTATTTGGAAAAAAAAAAAGGATAAACAGATTCATTAAATTATATGATTAAATC ACTGACCCAATAGAAAATTTATATAATAAAGCCAGAAAAAAAGTTGTAAAATATAGTTTA CAATAATTATTCACATTCAAGGCTGTACATCAATACATACAACAACGTGGCCCCGAACAT GAATTCAATTCAAATAATTCATATATTAGAATTTAAATAACACAGACTGAACTAGAGGCC AACAATAGCCAGCAAATAACCTTATATAAGAATAAAAATACAAAAGTGTATATCCTAGTA TCATTGCATTTGTTTTCATAAGTTCTTTTAATTTTTGCTTTGTCTGTACATCACAGTTAC AGCTACAGACCAGGTAGAGAATATTACGCATGTTCTGCTTGCCACCCACCTGAAAGACGT ACTAGTCATTTATCCTATTTTATGTTGTGAAGGAACAGCTACAGCAGCTCTAAATACTGT AACAATTTAATTTTGCCAACATCCAACTTACTTACAGTCGTAATAGAGGCTTTTACCAGC ACAATGACCCCTATTCGTAGTCATTCTGCCAGGTGGTTAGGCTATGTGGGTTACACAGCT TAAAAAAGGTCTGCCTTTTGTAAAAAGTTCTCTGTCCATAGTTTGGGGTTTCTTCCTATG GAAGAAAAGANTTACGGGGAAAATTCCGGTACCCCCTAGGCTTTGTAAATTGCCTACCAC AGGCCTCTCAACAAATGAAGTGCTCTTTGGTGAAACACTTTCTTCCTCACCCAGTCTTCC CAGGAAGCCCATTTAACAACTTGGTTTTTTGGCCACAAGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_031268 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031268.4, NP_112558.2 |
| RefSeq Size | 6873 bp |
| RefSeq ORF | 1290 bp |
| Locus ID | 5170 |
| UniProt ID | O15530 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Endometrial cancer, Focal adhesion, Insulin signaling pathway, mTOR signaling pathway, Non-small cell lung cancer, PPAR signaling pathway, Prostate cancer |
| Gene Summary | Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks three consecutive exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206746 | PDPK1 (Myc-DDK-tagged)-Human 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 2 |
CNY 3656.00 |
|
| RC206746L1 | Lenti ORF clone of Human 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 2, Myc-DDK-tagged |
CNY 6056.00 |
|
| RC206746L2 | Lenti ORF clone of Human 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RC206746L3 | Lenti ORF clone of Human 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC206746L4 | Lenti ORF clone of Human 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG206746 | PDPK1 (tGFP-tagged) - Human 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 2 |
CNY 5256.00 |
