SF3B5 (NM_031287) Human Untagged Clone
CAT＃: SC107691
SF3B5 (untagged)-Human splicing factor 3b, subunit 5, 10kDa (SF3B5)
CNY 1200.00
CNY 2950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SF3b10; Ysf3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC107691 sequence for NM_031287 edited (data generated by NextGen Sequencing)
ATGACTGACCGCTACACCATCCATAGCCAGCTGGAGCACCTGCAGTCCAAGTACATCGGC ACGGGCCACGCCGACACCACCAAGTGGGAGTGGCTGGKGAACCAACACCGCGACTCGTAC TGCTCCTACATGGGCCACTTCGACCTTCTCAACTACTTCGCCATTGCGGAGAATGAGAGC AAAGCGCGAGTCCGCTTCAACTTGATGGAAAAGATGCTTCAGCCTTGTGGACCGCCAGCC GACAAGCCCGAGGAGAACTGA Clone variation with respect to NM_031287.2 98 t=>k >OriGene 5' read for NM_031287 unedited
CACGAGGGTTACCTGCGTCCTCTGACCTGAGAGCGAAGGGGAAAGCGGCGAGATGACTGA CCGCTACACCATCCATAGCCAGCTGGAGCACCTGCAGTCCAAGTACATCGGCACGGGCCA CGCCGACACCACCAAGTGGGAGTGGCTGGTGAACCAACACCGCGACTCGTACTGCTCCTA CATGGGCCACTTCGACCTTCTCAACTACTTCGCCATTGCGGAGAATGAGAGCAAAGCGCG AGTCCGCTTCAACTTGATGGAAAAGATGCTTCAGCCTTGTGGACCGCCAGCCGACAAGCC CGAGGAGAACTGAGACTCTGCCTTACCACCGCAGTGCGGGGCACCTCTCCCAGCGTTTCT CCGGTTTGCCAATCCTCTTAAGTATTCCTGTCTCCAAAGGACCGGCTCTCCATGGCTCCT GCGCCTCGTGCTTTCCGCGTACAGAAGTGCTTGCCCGGGGAGTCCCGCCTGACCTGCCTT CATGTGGACCCTTAGAACAGCACTGGGAGACCAGCAGGACTCCTGAGAACTGTGCTGGTG GAGAGGTCCTAGAGCCGGCGAGCGTTTGAGAAGAGGGCATGGCGCTGGAGTGAGATGGGA TTTGGCGTCTCGTTTTTGGCTAATTGATTGTCATTGGCTTTTTCCATANAGTT >OriGene 3' read for NM_031287 unedited
CTATGGACGCGGCCGCATCTAAATNCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTTTGGGAAAATTCCTTTGTCTTTTATTTT TTCCAAAATCTATACTCTAATTTCTTTTTTTTTCCAGGGCCAGGCCCTTTTTTAAAAGGC TCGCCGGTTTTAGGAACCTTTCCACAAAAAAAGTTCTAAGGAGTCCTGTTGGTCCCCCAG GGGGGTTTTAAGGGACCACATGAAGGGGGGTAAGGGGGAACCCCCCGGGAAAGCACTTGG GTCCCCGGGAAGGCCCCAGGCGCGGGACCCAGGGAAACCCGGCCCTTTGGAAACGGGAAA CCTTAAGAGGATGGGCAAACCGGAAAAACGCGGGGAAAGGGGCCCCCCCCTGGGGGGGCA GGGCTTAGTCAAAAATCTCCTCGGGCTGGGGGGGGGGGGGCCCAAAAGGGGGAAGAATGT TTCCCACCAGGGGGAAGGGGACTCGCCCTTTGTTTTTATTCTCCGCATGGGGAGAAGGGG TCAAAAAGGGCAAAGGGCCCCTTTTAAAAAAAAAACAAGGCCCGGGGTTGGTTCACCACC CACCCCCACTGGGGGGGGTCGGAGAGGCCCGGGCCGATGTCCTTGAACTGCAAGGCCTCC CCCTGGTTATGGATCGCGAACCGCCCAGGTTTGGGGCCGCTTCCCCCTCCGCTCTAAGGT CAGAGGACGCAGGGACCCCCCGCGCCAGATGGGGGGGCGCCCTAAAAGANNCCNATACAA ACCCTTGCGGGTAACAAAAGAGCTCGGTAAATAACCCCCCACGGCCCCCCTACGCAATTG CGGAAAGGGGCGGGGTATTACAAATTGGGAAGTCCTGAATTCGGGGCAAAAAACTCCATG ATTTATGGGGGGGACTGAAATCCCGGGTGAACCCCTCCCCCCCTGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_031287 |
| Insert Size | 690 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031287.2, NP_112577.1 |
| RefSeq Size | 786 bp |
| RefSeq ORF | 261 bp |
| Locus ID | 83443 |
| UniProt ID | Q9BWJ5 |
| Protein Pathways | Spliceosome |
| Gene Summary | Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:27720643, PubMed:28781166). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200977 | SF3B5 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 5, 10kDa (SF3B5) |
CNY 1200.00 |
|
| RC200977L3 | Lenti ORF clone of Human splicing factor 3b, subunit 5, 10kDa (SF3B5), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC200977L4 | Lenti ORF clone of Human splicing factor 3b, subunit 5, 10kDa (SF3B5), mGFP tagged |
CNY 5890.00 |
|
| RG200977 | SF3B5 (tGFP-tagged) - Human splicing factor 3b, subunit 5, 10kDa (SF3B5) |
CNY 2800.00 |
