SRPK2 (NM_182691) Human Untagged Clone
CAT＃: SC107542
SRPK2 (untagged)-Human SRSF protein kinase 2 (SRPK2), transcript variant 2
CNY 5048.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SFRSK2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_182691 edited
ATGTCAGTTAACTCTGAGAAGTCGTCCTCTTCAGAAAGGCCGGAGCCTCAACAGAAAGCT CCTTTAGTTCCTCCTCCTCCACCGCCACCACCACCACCACCGCCACCTTTGCCAGACCCC ACACCCCCGGAGCCAGAGGAGGAGATCCTGGGATCAGATGATGAGGAGCAAGAGGACCCT GCGGACTACTGCAAAGGTGGATATCATCCAGTGAAAATTGGAGACCTCTTCAATGGCCGG TATCATGTTATTAGAAAGCTTGGATGGGGGCACTTCTCTACTGTCTGGCTGTGCTGGGAT ATGCAGGGGAAAAGATTTGTTGCAATGAAAGTTGTAAAAAGTGCCCAGCATTATACGGAG ACAGCCTTGGATGAAATAAAATTGCTCAAATGTGTTCGAGAAAGTGATCCCAGTGACCCA AACAAAGACATGGTGGTCCAGCTCATTGACGACTTCAAGATTTCAGGCATGAATGGGATA CATGTCTGCATGGTCTTCGAAGTACTTGGCCACCATCTCCTCAAGTGGATCATCAAATCC AACTATCAAGGCCTCCCAGTACGTTGTGTGAAGAGTATCATTCGACAGGTCCTTCAAGGG TTAGATTACTTACACAGTAAGTGCAAGATCATTCATACTGACATAAAGCCGGAAAATATC TTGATGTGTGTGGATGATGCATATGTGAGAAGAATGGCAGCTGAGGCCACTGAGTGGCAG AAAGCAGGTGCTCCTCCTCCTTCAGGGTCTGCAGTGAGTACGGCTCCACAGCAGAAACCT ATAGGAAAAATATCTAAAAACAAAAAGAAAAAACTGAAAAAGAAACAGAAGAGGCAGGCT GAGTTATTGGAGAAGCGCCTGCAGGAGATAGAAGAATTGGAGCGAGAAGCTGAAAGGAAA ATAATAGAAGAAAACATCACCTCAGCTGCACCTTCCAATGACCAGGATGGCGAATACTGC CCAGAGGTGAAACTAAAAACAACAGGATTAGAGGAGGCGGCTGAGGCAGAGACTGCAAAG GACAATGGTGAAGCTGAGGACCAGGAAGAGAAAGAAGATGCTGAGAAAGAAAACATTGAA AAAGATGAAGATGATGTAGATCAGGAACTTGCGAACGTAGACCCTACGTGGATAGAATCA CCTAAAACCAATGGCCATATTGAGAATGGCCCATTCTCACTGGAGCAGCAACTGGACGAT GAAGATGATGATGAAGAAGACTGCCCAAATCCTGAGGAATATAATCTTGATGAGCCAAAT GCAGAAAGTGATTACACATATAGCAGCTCCTATGAACAATTCAATGGTGAATTGCCAAAT GGACGACATAAAATTCCCGAGTCACAGTTCCCAGAGTTTTCCACCTCGTTGTTCTCTGGA TCCTTAGAACCTGTGGCCTGCGGCTCTGTGCTTTCTGAGGGATCACCACTTACTGAGCAA GAGGAGAGCAGTCCATCCCATGACAGAAGCAGAACGGTTTCAGCCTCCAGTACTGGGGAT TTGCCAAAAGCAAAAACCCGGGCAGCTGACTTGTTGGTGAATCCCCTGGATCCGCGGAAT GCAGATAAAATTAGAGTAAAAATTGCTGACCTGGGAAATGCTTGTTGGGTGCATAAACAC TTCACGGAAGACATCCAGACGCGTCAGTACCGCTCCATAGAGGTTTTAATAGGAGCGGGG TACAGCACCCCTGCGGACATCTGGAGCACGGCGTGTATGGCATTTGAGCTGGCAACGGGA GATTATTTGTTTGAACCACATTCTGGGGAAGACTATTCCAGAGACGAAGACCACATAGCC CACATCATAGAGCTGCTAGGCAGTATTCCAAGGCACTTTGCTCTATCTGGAAAATATTCT CGGGAATTCTTCAATCGCAGAGGAGAACTGCGACACATCACCAAGCTGAAGCCCTGGAGC CTCTTTGATGTACTTGTGGAAAAGTATGGCTGGCCCCATGAAGATGCTGCACAGTTTACA GATTTCCTGATCCCGATGTTAGAAATGGTTCCAGAAAAACGAGCCTCAGCTGGCGAATGC CTTCGGCATCCTTGGTTGAATTCTTAG >OriGene 5' read for NM_182691 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGAGCGAGTGGAGGC TGCAGCCCAGCTCGTCTCGGCGCCCGCGTCGCCGTCGCGAAGCCCCCCGCCCCGCTTCCG CCGCGTCGGAATGAGCTCCCGGAAAGTGCTGGCCATTCAGGCCCGAAAGCGGAGGCCGAA AAGAGAGAAACACTCCGAAAAAAAATCAAGCAGAAGATTGAGCTGCTGATGTCAGTTAAC TCTGAGAAGTCGTCCTCTTCAGAAAGGCCGGAGCCTCAACAGAAAGCTCCTTTAGTTCCT CCTCCTCCACCGCCACCACCACCACCACCGCCACCTTTGCCAGACCCCACACCCCCGGAG CCAGAGGAGGAGATCCTGGGATCAGATGATGAGGAGCAAGAGGACCCTGCGGACTACTGC AAAGGTGGATATCATCCAGTGAAAATTGGAGACCTCTTCAATGGCCGGTATCATGTTATT AGAAAGCTTGGATGGGGGCACTTCTCTACTGTCTGGCTGTGCTGGGATATGCAGGGGAAA AGATTTGTTGCAATGAAAGTTGTAAAAAGTGCCCAGCATTATACGGAGACAGCCTTGGAT GAAATAAAATTGCTCAAATGTGTTCGAGAAAGTGATCCCAGTGACCCAAACAAAGACATG GTGGTCCAGCTCATTGACGACTTCAAGATTTCAGGCATGAATGGGATACATGTCTGCATG GTCTTCGAAGTACTTGGCCACCATCTCCTCAAGTGGATCATCAAATCCAACTATCAAGGC CTCCCAGTACGTTGTGGTGAAGAGTATCATTCGACAGGTCCTCCAAGGGTAGATTACTTA CACAGNNTAGTGCAGATCATTCATACTGACATANAGCCCGGAAAATATCTTGAGTGTNGT GGATGATGCATATGTGAGAAGAATGGNCAGCTGAGCCACTGAGTGCAAAAAGCAGT >OriGene 3' read for NM_182691 unedited
CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAGCTTTTCTGAAATTTGAGTC CCCCTCCCATATTTAATGGAAAGTAACATTTACAGGGTGCATTTACATACACTATAATAC AGGAATGATGTCCCTTTGCCAGAAGATAAAATTGTACATTTCCTTGCTATTTCTTGGTTC CAACTTGATAATTTCTTAAGATTGTAAATTATATAGTACTCAGCTAAAATATTTCTTCAT AAATAGTTACAAAACCTGCCAAAACACAAAGGGGAAAGTATTTTTCATTCAAAAAAATGA CATTTGAATGTCACACAACACAAGAAACAATGCTTAGAGACATGTTATTGTTTCCAGAAG AAAATGGTCAGTAAACTACTTAGCTGAAGACAAAAGACTACCCTTCCCCAGGATCACAGT GCACAAAAAGCAAAATGTCAAACAACAGTACCTCAAAGCAAAATAAAGGTCTGAGGATGA AGCCAGCTCACTTGTAATCCTGTTAAAGAATGAGAGTCACCGTTTAGGTCCAATGTACTG GGAACATTTGCTAGCTCAGAATGCAATATTGGTAGAATTTGCTAAGAATTCAACCAAGGA TGCCGAAGGCATTCGCCAGCTGAGGCTCGTTTTTCTGGAACCATTTCTAACATCGGGATC AGGAAATCTGTAAACTGTGCAGCATCTTCATGGGGCCAGCCATACTTTTCCACAAGTACA TCAAAGAGGCTCCAGGGCTTCAGCTTGGTGATGTGTCGCAGTTCTCCTCTGCGATTGAAG AATTCCCGAGAATATTNTCCAGATAGAGCAAAGTGCCTTGGAATACTGCCTANCAGCTCT ATGATGTGCGCTACGTGGCCTCCGCCCCTGGAATATCTTCCCCAAATGTGGTTCAAACAA TAATCTCCCGTTGCAGCTCAATGCCTACAGCCCGGCTTCCATGTCGTAGGGCGCTGTACC CGCTCTATTAAACCCTTCGCACGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_182691 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182691.1, NP_872633.1 |
| RefSeq Size | 3737 bp |
| RefSeq ORF | 2067 bp |
| Locus ID | 6733 |
| UniProt ID | P78362 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate first exon compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Paraquat Modulates Alternative Pre-mRNA Splicing by Modifying the Intracellular Distribution of SRPK2
,null,
PLoS ONE
,PubMed ID 23613995
[SRPK2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205134 | SRPK2 (Myc-DDK-tagged)-Human SRSF protein kinase 2 (SRPK2), transcript variant 2 |
CNY 5040.00 |
|
| RC205134L1 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, Myc-DDK-tagged |
CNY 7440.00 |
|
| RC205134L2 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RC205134L3 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC205134L4 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG205134 | SRPK2 (tGFP-tagged) - Human SRSF protein kinase 2 (SRPK2), transcript variant 2 |
CNY 6640.00 |
|
| SC322514 | SRPK2 (untagged)-Human SRSF protein kinase 2 (SRPK2), transcript variant 2 |
CNY 5048.00 |
|
| SC323336 | SRPK2 (untagged)-Kinase deficient mutant (K121M) of Human SFRS protein kinase 2 (SRPK2), transcript variant 2 |
CNY 5048.00 |
