BMS1P5 (BC020053) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC020053, the custom clone sequence may differ by one or more nucleotides GTGAGCAGGTTCGGTGCCGCGGGCTGGGATTAGAATTACTTGTTATTGGTAAATAGCCACTATGGAGACT AAGGACCAGAAGAAACAAAGAAAGAAAAATAGTGGACCCAAAGCTGCAAAGAAAAAGAAGCGGCATCTGC AGGATCTCCAGCTAGGAGACGAAGAAGATGCCTGGAAGAGAAATCCCAAAGCTTTTGCATTTCAGTCTGC TGTGTGGATGGCTCGATCCTTTCACAGGACTCAGGATTTGAAGACAAAAAAGCATCATATTCCAGTGGTT GATCGAACTCCACTAGAGCCCCCACCAATAGTGGTAGTGGTGATGGGGCCTCCAAAGTTGGAAAGAGCAC TTTGATACAATGCCTCATTCGGAACTTCACCCGGCAGAAGTTGACCGAGATCAGAGGCCCTGTGATGATC GTGTCAGGTAAAAAGCTCCGACTCACCATTATTGAATGTGGGTGTGACATTAACATGATGATTGATCTGG CTGAAGTAGCAGATCTGGTACTGATGCTTATAGATGCCAGCTTTGGGTTTGAAATGGAAACGTTTGAGTT TCTAAACATCTGTCAAGTACATGGCTTTCCTAAAATTATGGGAGTTCTCACCCACCTCGACTTCTTCAAA CACAATAAGCAACTGAAGAAGACAAAGAAGCGATTAAAACACAGGTTCTGGACAGAAGTTTACCTGGTTG CCAAGCTGTTCTGCCTTTCTGGAATGGTGCATGGAGAATATCAAAACCAAGAAATGCACAATCTGGGCCA TTTTATTACAGTTATGAAGTTTAGGCCTCTCACATGGCAGACTTCTCATCCTTATATCCTGGCAGACAGG GTGGAAGATTTGACAAACCCAGAGGATATCCAAACAAACATCAGATGTGACCAGCAGATGTTACTTTACG GTTATTTAAGAGGAGCGCACTTGAAAAATAAAAGCCAAATTCACATGCCAGGACGAGGTGGGGCCCACCC ATGAGCTGGTCCAGAGTCTCATCTCCACCCACTCCACCATTGATGCCAAGATGGCTTCAAGTCGAGTGAT GTTGTTTTCTGATTCCAAGCCACTTGGGTCAGAGGCTATAGATAATCAAGGGGACGAGTTCTCACTATGT TGCCCAGGCTGGCCTCGAACTCCTGGGCTCAAGCAATCCTCCTGCGTTGGCCTCCCAAAGTCCTGGGATT ACAGGTGTAAGCCACTACGGCTGGCCTATTATTGCTGTATTCTAACTTATTCATCTCCTATGTGTCAGAG ATGGCTTTGGATTCCCCGTTCTGTGTGCTGTTGTCTGGCTCCTGAACCCAGCTGTAGAGGTGTGTCAATC CCAACTGGTGAAGTACTGAGAGGAAGCTACACAAAAGGCAGCAAAGTATTAGTGAGTGGTGTCATTGAGC AAACACGAAGTCGGGCTCATCCAAGGATGAGATTTTGCCAGAGAAAGGACGAGCAGCAAGTCAGGGAGCT TAAGCGTCAGCCTCGAGTTTGACCAGCAACAGGGGTCGGTGTGTCCCTCTGAATCTGAGATCTATGAGGC AGGAGCTGGGGACAGGATGGCAGGAGCGCCCATGGCTGCTGCTGTGCAGCCTGCTGAGGTGACCGTTGAA GTTGGTGAGGACCTCCACATGCACCACATTCGTGACCAGGAGATGCCTGAAGCTTTGGAGTTTAGCCTTT CTGCCAATCCAGAGGCAAGCACAATATTCCAGAGGAACTCTCAAACAGATGCTTTGGAGTTTAACCCTTC TGCCAATCCAGAGGCAAGCACAATATTCCAGAGGAACTCTCAAACAGATGTTGTAGAAATAAGAAGAAGC AACTGTACAAACCATGTATCTACTGAGCGTTTCAGTCAACAATACAGCTCGTGTTCGACAATATTCCTTG ATGACAGCACAGCCAGCCAGCATTATCTTACAATGACAATAATATCTGTGACCTTGGAGATACCTCATCA TATCACACAAAGAGATGCAGATAGATCTTTGAGCATACCTGATGAACAGTTACACTCATTTGCGGTTTCC ACCGTGCACATTACGAAGAACAGAAATGGAGGTGGGAGTTTAAATAACTATTCCTCCTCCATTCCATCGA CTCCCAGCACCAGCCAGGAGGACCCTCAGTTCAGTGTTCCTCCCACTGCCAACACACCCACCCCCGTTTG CAAGCGGTCCATGCGCTGGTCCAACCTGTTTACATCTGAGAAAGGGAGTCACCCAGACAAAGAGAGGAAA GCCCCGGAGAATCATGCTGACACCATCGGGAGCGGCAGAGCCATCCCCATTAAACAGGGCATGCTCTTAA AGCGAAGTGGGAAATGGCTGAAGACATGGAAAAAGAAATACGTCACCCTGTGTTCCAATGGCGTGCTCAC CTATTATTCAAGCTTAGGTGATTATATGAAGAATATTCATAAAAAAGAGATTGACCTTCGGACATCTACC ATCAAAGTCCCAGGAAAGTGGCCATCCCTAGCCACATCGGCCTGTGCACCCATCTCCAGCTCTAAAAGCA ATGGCCTATCCAAGGACATGGACACCGGGCTGGGTGACTCCATATGCTTCAGCCCCAGTATCTCCAGCAC CACCAGCCCCAAGCTCAACCCGCCCCCCTCTCCTCATGCCAATAAAAAGAAACACCTAAAGAAGAAAAGC ACCAACAACTTTATGATTGTGTCTGCCACTGGCCAAACATGGCACTTTGAAGCCACGACGTATGAGGAGC GGGATGCCTGGGTCCAAGCCATCCAGAGCCAGATCCTGGCCAGCCTGCAGTCATGCGAGAGCAGTAAAAG CAAGTCCCAGCTGACCAGCCAGAGCGAGGCCATGGCCCTGCAGTCGATCCAAAACATGCGTGGGAACGCC CACTGTGTGGACTGTGAGACCCAGAATCCTAAGTGGGCCAGTTTGAACTTGGGAGTCCTCATGTGTATTG AATGCTCAGGAATCCACCGCAGTCTTGGCACCCGCCTTTCCCGTGTGCGATCTCTGGAGCTGGATGACTG GCCAGTTGAGCTCAGGAAGGTTATGTCATCTATTGGCAATGACCTAGCCAACAGCATCTGGGAAGGGAGC AGCCAGGGGCAGACAAAACCCTCAGTAAAGTCCACGAGGGAAGAGAAGGAACGGTGGATCCGTTCCAAAT ATGAGGAGAAGCTCTTTCTGGCCCCACTACCCTGCACTGAGCTGTCCCTGGGCCAGCACCTGCTGCGGGC CACCGCTGATGAGGACCTGCAGACAGCCATCCTGCTGCTGGCACATGGCTCCCGTGAGGAGGTGAACGAG ACCTGTGGGGAGGGAGACGGCTGCACGGCGCTCCATCTGGCCTGCCGCAAGGGGAATGTGGTCCTGGCAC AGCTCCTGATCTGGTACGGGGTGGACGTCATGGCCCGAGATGCCCACGGGAACACAGCGCTGACCTACGC CCGGCAGGCCTCCAGCCAGGAGTGCATCAACGTGCTTCTGCAGTACGGCTGCCCCGACGAGTGCGTGTAG TATCTGTTTTATTTGACTGCAGTCTCCTTGGTGCAAAAACAAAATGGGAAAAATAAGGATAACTCAGAAT TTCAAAAGGAAATCACAAATTCAGCTAGTAATAGCATTTTCAGTACTTTTCATAAACTAAGTAAATACAC AAAATGTTGATTTTTCTGACCATAAGACATATTTTATGTCCTTTTGCCAAGGTGGATGTGTTAGTCTCAG GCCCTCCTGGCCACATTGCCCAAGTCACACAGGCTTCTGTATTATGTATTTAGATAAAATGTGTGAAAAT ATATTTGAAAAAAAAGTTCATAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020053 unedited AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGTTGTGAGCAAGTT CGGTGCCGCGGGCTGGGGTAGGCGGCAGTGGGGTCCCTGGCTTTGGGGCACTGGGAAGAT CGCGAGTGGGAAGGTCCTCCCGCGGTCCTCCGGGTCATCTTTCCTCCTGAGGAGCAGCAT ATTTCGCATCTGCCGGAGATTAGAATTACTTGTTATTGGTAAATAGCCACTATGGAGACT AAGGACCAGAAGAAACAAAGAAAGAAAAATAGTGGACCCAAAGCTGCAAAGAAAAAGAAG CGGCATCTGCAGGATCTCCAGCTAGGAGACGAAGAAGATGCCTGGAAGAGAAATCCCAAA GCTTTTGCATTTCAGTCTGCTGTGTGGATGGCTCGATCCTTTCACAGGACTCAGGATTTG AAGACAAAAAAGCATCATATTCCAGTGGTTGATCGAACTCCACTAGAGCCCCCACCAATA GTGGTAGTGGTGATGGGGCCCTCCAAAGNTGGAAAGGAGCACTTTGATACAATGCCTCAT TCGGAACCTTCACCCCGCAGATGTTGACCAAGAATCAGAGGCCCTGTGATGATCGTGTCA GGTAAAAAGCTCCGACTCACCATTATTGATGTGGGTGTGACATTACATGATGATTGGTCT GCCTGGAGTAACAAATCTGGACTGATGCTAATAGAAGCCCACTTTGGTTAGAAAAGGGAA CGTTTGATTTCTAAACATCTGGAAGACCTGGGTTTCCTAAATTTGGGAGTCCTCACCACC TCGATTTTTAAACAAATAG
Restriction Sites	NotI-NotI
ACCN	BC020053
Insert Size	4100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020053.1
RefSeq Size	3816 bp
RefSeq ORF	1266 bp
Locus ID	399761

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