MRPL32 (NM_031903) Human Untagged Clone
CAT＃: SC107191
MRPL32 (untagged)-Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein
CNY 2400.00
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bMRP-59b; HSPC283; L32mt; MRP-L32 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC107191 sequence for NM_031903 edited (data generated by NextGen Sequencing)
ATGGCGCTGGCCATGCTGGTCTTGGTGGTTTCGCCGTGGTCTGCGGCCCGGGGAGTGCTT CGAAACTACTGGGAGCGACTGCTACGGAAGCTTCCGCAGAGCCGGCCGGGCTTTCCCAGT CCTCCGTGGGGACCAGCATTAGCAGTACAGGGCCCAGCCATGTTTACAGAGCCAGCAAAT GATACCAGTGGAAGTAAAGAGAATTCCAGCCTTTTGGACAGTATCTTTTGGATGGCAGCT CCCAAAAATAGACGCACCATTGAAGTTAACCGGTGTAGGAGAAGAAATCCGCAGAAGCTT ATTAAAGTTAANAACAACATAGACGTTTGTCCTGAATGTGGTCACCTGAAACAGAAACAT GTCCTTTGTGCCTACTGCTATGAAAAGGTGTGCAAGGAGACTGCAGAAATCAGACGACAG ATAGGGAAGCAAGAAGGGGGCCCTTTTAAGGCTCCCACCATAGAGACTGTGGTGCTGTAC ACAGGAGAGACACCGTCTGAACAAGATCAGGGCAAGAGGATCATTGAACGAGACAGAAAG CGACCATCCTGGTTCACCCAGAATTGA Clone variation with respect to NM_031903.2 312 g=>n;483 g=>a >OriGene 5' read for NM_031903 unedited
AATTAAAAATTGAACACGACTCATATAGGCGGACGCGAATTGGACGAGGGGAAAAGGCGC TGGCCATGCTGGTCTTGGTGGTTTCGCCGTGGTCTGCGGCCCGGGGAGTGCTTCGAACTA CTGGGAGCGACTGCTACGGAAGCTTCCGCAGAGCCGGCCGGGCTTTCCCAGTCCTCCGTG GGGACCAGCATTAGCAGTACAGGGCCCAGCCATGTTTACAGAGCCAGCAAATGATACCAG TGGAAGTAAAGAGAATTCCAGCCTTTTGGACAGAATCTTTTGGATGGCAGCTCCCAAAAA TAGACGCACCATTGAAGTTAACCGGCGTAGGAGAAGAAATCCGCAGAAGCCTTATTAAAG TTAAGGTAATGCATTGATTTTTGTGGGTGTGCTTTTCCTCTAAGTCTCCATTGAATAAGC TCATGGTCCCATCCTATAGCCTAGGAATAAATTACTTCTCAGACTATATGTGCCTACTTT CCTCTGGCCTAAATGAAATACCAGTATTCTCATTTATATACATAATGAGAAGGCTAGAGC CTGCTGTCCTTTAGCCTGAGTATTAGAAACACAGCCCAGGAAGGAGTGAGCTTGGAGACT GGGACCGGAACCAGGCTGTGAAGTGCCTACATGACTTTACGAACTGAGAGCTGGGTCAAC TTTGAGCTGTGTGAGGCCTGAACTACCGAAGAGATACCATCCTACCTATTGCACAACCTT AAAATTAAGGGCTTGAAGCCGTAGGCTATCCTGAAACCTTTCGGTTTCCACACCCCCTTC CCACCACCGGTCTAATTTGTTTAGCATTTTCTACACAAACACCATGTAAGTAGACCTCTT ACACTAGTCCCTCTATTGCTTAACACTCAATACAGGGTCAAAAAGGGAAACTACAGGCGC CTATGGGTTAGATAAACCACACCATAGCCTATAAGCCTCCCCAGCAAAAAAAACA >OriGene 3' read for NM_031903 unedited
CCCAAATCTTGNCCGCGGCCGCAATCTACGAGCCGCTTTTTTTTTTTTTTTTTTGCTATG AATTTTAAATTTTTTATTTTTGTTCTGGAAATTTTATGTTTACTTAAAACCTTGTATATA ATAGAAACATAATCCATTGAGCCTTTGGATAAACAAAATTTTCGGAGTTTACTCATAATA TATTCTTCAAATTATCCCACACAAAACTGCTTAGAAAGAATGTGTTAAACTATACTGATG ATTTAAAAACAAGCACAACAACGTAAAGAATCTTCCTCTATTTCAGGAGAAATGATTTAC TGTGAAGTTATCCTTTTAACATCTTTGGTGTCAATTCTGGGTGAACCAGGATGGTCGCTT TCTGTCTCGTTCAATGATCCTCTTGCCCTGATCTTGTTCAGACGGTGTCTCTCCTGTGTA CAGCACCACAGTCTCTATGGTGGGAGCCTTAAAAGGGCCCCCTTCTTGCTTCCCTATCTG TCGTCTGAATTCTGCAGTCTCCTTGCACACCTTTTCATAGCAGTAGGCACAAAGGACATG TTTCTGTTTCAGGTGACCACATTCAGGACAAACGTCTATGTTGTTCTTTAAAACAATAAA CAAAAACGTATTTTAACATGAGGCAATATAACGAACAAATAACCACTGACAGGAGCAATG CTTACATTTTAACTTTCTTAGGATGTTCAGGCTACATGAGGTCATCATACACCATCCAGT TTCACCTCACTTTAACAGGAAGACTGAAGACCAGCACACTGTGTTTGAATTAGAGAAAAC TAGTACTAGAATCCGCCAACCTGAATCTGGTATCTTTATTAAACACAGCGCTGTCGTTAT GTAAAAAAGAAAAAGAAAACCCTTTTCCCCTACTCCCAAACTAAAATCACAAAGGAAATT GCCACCTAAGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_031903 |
| Insert Size | 3110 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031903.1, NP_114109.1 |
| RefSeq Size | 903 bp |
| RefSeq ORF | 567 bp |
| Locus ID | 64983 |
| UniProt ID | Q9BYC8 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204559 | MRPL32 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein |
CNY 2400.00 |
|
| RC204559L3 | Lenti ORF clone of Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC204559L4 | Lenti ORF clone of Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5890.00 |
|
| RG204559 | MRPL32 (tGFP-tagged) - Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein |
CNY 4370.00 |
|
| SC322462 | MRPL32 (untagged)-Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein |
CNY 2400.00 |
