STT3B (NM_178862) Human Untagged Clone
CAT＃: SC107172
STT3B (untagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B)
CNY 9088.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDG1X; SIMP; STT3-B |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_178862, the custom clone sequence may differ by one or more nucleotides
ATGGCGGAGCCCTCGGCCCCGGAGAGCAAGCACAAGTCGTCCCTCAACTCGTCCCCGTGGAGTGGCCTCA TGGCCCTGGGAAACAGCCGGCACGGCCACCACGGGCCCGGGGCCCAGTGCGCGCACAAGGCGGCGGGCGG CGCGGCGCCGCCGAAGCCGGCCCCGGCGGGGCTGTCCGGGGGGCTGTCGCAGCCGGCTGGGTGGCAGTCG CTTCTCTCCTTCACCATCCTCTTCCTGGCCTGGCTTGCCGGCTTCAGCTCGCGCCTCTTCGCCGTCATCC GCTTCGAAAGCATCATCCACGAGTTCGACCCGTGGTTTAACTATAGATCAACACATCATCTTGCATCTCA TGGGTTCTATGAATTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCACTAGGAAGAATAGTAGGTGGT ACTGTTTACCCAGGGTTGATGATAACCGCTGGCCTTATTCATTGGATTTTAAATACATTGAACATAACTG TTCACATAAGAGACGTATGTGTGTTCCTTGCACCAACTTTTAGCGGCCTTACATCTATATCTACTTTCCT GCTTACAAGAGAACTTTGGAACCAAGGAGCAGGACTTTTAGCTGCTTGTTTTATTGCTATTGTACCAGGC TACATATCTCGGTCAGTAGCTGGATCCTTTGATAATGAAGGCATTGCTATTTTTGCACTTCAGTTCACAT ACTATTTATGGGTAAAATCTGTAAAAACTGGGTCAGTTTTTTGGACAATGTGCTGCTGCTTATCCTATTT CTATATGGTCTCTGCTTGGGGTGGTTATGTATTTATCATCAATCTTATTCCACTGCATGTATTTGTGTTG TTACTGATGCAGAGATACAGCAAAAGAGTCTACATAGCATATAGCACTTTCTACATTGTGGGTTTAATAT TATCAATGCAGATACCTTTTGTGGGATTCCAGCCAATCAGAACAAGTGAACACATGGCAGCTGCAGGTGT CTTTGCATTGCTGCAAGCTTATGCTTTCTTGCAGTATCTGAGAGACCGATTAACAAAACAAGAGTTCCAG ACCCTTTTCTTTTTGGGTGTATCACTAGCTGCAGGTGCTGTGTTCCTTAGTGTCATCTATTTGACTTATA CAGGTTACATTGCACCATGGAGTGGCAGGTTTTATTCATTGTGGGATACTGGGTATGCAAAAATACACAT TCCAATTATTGCATCAGTGTCTGAGCATCAACCTACGACTTGGGTGTCTTTCTTCTTTGATCTACATATT CTTGTATGTACCTTCCCAGCAGGCCTTTGGTTCTGCATCAAAAATATCAACGATGAAAGAGTATTTGTTG CTCTATATGCAATCAGTGCTGTCTACTTTGCTGGAGTGATGGTGCGACTGATGTTGACTTTGACTCCAGT CGTGTGTATGCTGTCTGCAATTGCCTTTTCAAATGTTTTTGAGCACTATTTGGGGGATGACATGAAAAGG GAAAATCCACCTGTGGAGGACAGCAGTGATGAGGATGACAAAAGAAACCAAGGAAATTTGTATGATAAGG CAGGTAAAGTGAGGAAACATGCAACTGAACAGGAAAAAACTGAAGAGGGATTAGGCCCTAATATAAAAAG CATTGTCACCATGTTGATGCTGATGCTATTGATGATGTTTGCTGTCCACTGTACCTGGGTCACAAGCAAT GCCTACTCTAGTCCAAGTGTAGTCCTGGCCTCATACAATCATGATGGCACCAGGAATATCTTAGATGATT TTAGAGAAGCTTACTTTTGGCTAAGGCAAAATACAGATGAACATGCACGAGTAATGTCTTGGTGGGATTA TGGCTATCAGATAGCTGGAATGGCTAATAGAACTACGTTGGTGGATAATAACACCTGGAATAACAGCCAC ATAGCACTGGTGGGAAAAGCTATGTCTTCTAATGAAACAGCAGCCTATAAAATCATGAGGACTCTAGATG TAGATTATGTTTTGGTTATTTTTGGAGGGGTTATTGGCTATTCTGGTGATGATATCAACAAATTTCTCTG GATGGTTAGGATAGCTGAAGGAGAACATCCCAAAGACATTCGGGAAAGTGACTATTTTACCCCACAGGGA GAATTCCGTGTAGACAAAGCAGGATCCCCTACTTTGTTGAATTGCCTTATGTATAAAATGTCATACTACA GATTTGGAGAAATGCAGCTGGATTTTCGTACACCCCCAGGTTTTGACCGAACACGTAATGCTGAGATTGG AAATAAGGACATTAAATTCAAACATTTGGAAGAAGCCTTTACATCAGAACACTGGCTTGTTAGGATATAT AAAGTAAAAGCACCTGATAACAGGGAGACATTAGATCACAAACCTCGAGTCACCAACATTTTCCCAAAAC AGAAGTATTTGTCAAAGAAGACTACCAAAAGGAAGCGTGGCTACATTAAAAATAAGCTGGTTTTTAAGAA AGGCAAGAAAATATCTAAGAAGACTGTTTAA >OriGene 5' read for NM_178862 unedited
GGCCGCGAAATTCGCACGAGGGGAGAGCTAGACCCGCCGCCGGGGCACAACATGGCGGAG CCCTCGGCCCCGGAGAGCAAGCACAAGTCGTCCCTCAACTCGTCCCCGTGGAGTGGCCTC ATGGCCCTGGGAAACAGCCGGCACGGCCACCACGGGCCCGGGGCCCAGTGCGCGCACAAG GCGGCGGGCGGCGCGGCGCCGCCGAAGCCGGCCCCGGCGGGGCTGTCCGGGGGGCTGTCG CAGCCGGCTGGGTGGCAGTCGCTTCTCTCCTTCACCATCCTCTTCCTGGCCTGGCTTGCC GGCTTCAGCTCGCGCCTCTTCGCCGTCATCCGCTTCGAAAGCATCATCCACGAGTTCGAC CCGTGGTTTAACTATAGATCAACACATCATCTTGCATCTCATGGGTTCTATGAATTTTTA AATTGGTTTGATGAAAGAGCATGGTATCCACTAGGAAGAATAGTAGGTGGTACTGTTTAC CCAGGGTTGATGATAACCGCTGGCCTTATTCATTGGATTTTAAATACATTGAACATAACT GTTCACATAAGAGACGTATGTGTGTTCCTTGCACCAACTTTTAGCGGCCTTACATCTATA TCTACTTTCCTGCTTACAAGAGAACTTTGGAACCAAGGAGCAGGACTTTTAGCTGCTTGT TTTATTGCTATTGTACCAGGCTACATATCTCGGTCAGTAGCTGGGATCCTTTGATAATGA AGGCATTGCTATTTTTGCACTTCAGTTACATACTATNTATGGGNTAAATCTGTAAAACTG GGGTCAGTTTTTGGGACAATGTGCTGCTGCTTATCC >OriGene 3' read for NM_178862 unedited
GCAATGGGGTGATGGGTTCACCAGGGCATGCCCACCCGGGTATCTGTTTCAGGAAAACAG CCTATTGACCGCGGCCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAAATTTG AATTAACTTTATTTCCCCTACAGTCAACAACTTCTCTGCATTGAATTTTAAAGCAACAAA GTAAAATAAAATCCCCCAAATGACAAGGTATCAGAAATCCAGACTTCGTTACACTCTATT TGTGAAAATGTTCAGCCATTTTATTGTTCTGTAAAGGAACCATCATCTGGGTCAAAAATG AATTCTATAAATCAAAGAATATTCTACACCTTGGAAAAGAAAATCAGCAGTAACATTCTC ACTGAATATTGTAAATTACATTTTTCTTCATAAAAGGTACATACTATTCTGCACTTTTCC ACCAAAAGCAGTGGTGTGTTATGCTTGTTATATAAAAAAAAGTTATATCCTGTGGCAGGA AAAACCCTTTCTCTTTCACTTTTACTAAACAACTGGAGAAAATGTTCAAGTCTGTATAAA GTTGCCTATAAGCTGGAAAGTGAACTTGTTCAATCTCCATTTACATTTTAGTGCATTTTT TTGACAATTGTCACATTTTTTTACANAAGTAAGAAAATGCATATAGCACTAAAGAGTGTT TCATCAAATGCTTAAAGGATTAAAAAAAATGGGAGCAGAGAACAAAATCATTGTGAATGG ATGAACCTGTTGTAAATGAAAAAAGTCCAGGCAAAGTTGTTTCCAGTCTTTTGTCCCTTT TATGAGTCCCAAAAATGAACTTTTGGTCCCTGTCCCTTTTGGGGGTTTTTTCCTTAAACT TTTGCGGGAAACCAAGTGCTAAACTTCTTAACAAGATACCAAACTCCCTTCTGGTTAGAC ACTTCTTTTTGTTTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_178862 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178862.1, NP_849193.1 |
| RefSeq Size | 2481 bp |
| RefSeq ORF | 2481 bp |
| Locus ID | 201595 |
| UniProt ID | Q8TCJ2 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | The protein encoded by this gene is a catalytic subunit of a protein complex that transfers oligosaccharides onto asparagine residues. Defects in this gene are a cause of congenital disorder of glycosylation Ix (CDG1X). [provided by RefSeq, Jun 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mutations in STT3A and STT3B cause two congenital disorders of glycosylation
,Shiteshu Shrimal, Bobby G. Ng, Marie-Estelle Losfeld, Reid Gilmore, and Hudson H. Freeze,
Hum. Mol. Genet., Jul 2013; 10.1093/hmg/ddt312.
,PubMed ID 23842455
[STT3B]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218829 | STT3B (Myc-DDK-tagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B). Note: ORF is codon optimized |
CNY 8650.00 |
|
| RC218829L3 | Lenti-ORF clone of STT3B (Myc-DDK-tagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B). Note: ORF is codon optimized |
CNY 10550.00 |
|
| RC218829L4 | Lenti-ORF clone of STT3B (mGFP-tagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B). Note: ORF is codon optimized |
CNY 10550.00 |
|
| RG218829 | STT3B (tGFP-tagged) - Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B). Note: ORF is codon optimized |
CNY 9500.00 |
