LIN37 (BC009071) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	F25965; lin-37; lin-37 homolog; lin-37 homolog (C. elegans); MGC9505; protein F25965; ZK418.4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC009071, the custom clone sequence may differ by one or more nucleotides ATGTTCCCTGTGAAGGTGAAAGTGGAGAAATCAGAGCTGGAGATGGCCAAAGCCCGGAACCAACTGGATG CTGTCTTGCAGTGTCTGCTGGAGAAGAGTCACATGGACAGGGAGCGTCTGGATGAGGAAGCTGGGAAAAC ACCCTCAGACACCCACAATAAGGACTGCTCCATCGCAGCCACTGGCAAAAGGCCATCTGCCCGCTTCCCC CACCAGCGGAGGAAGAAGAGGAGGGAGATGGATGATGGGCTGGCTGAGGGAGGGCCGCAGCGATCCAACA CATATGTGATCAAGCTGTTCGACCGGAGCGTGGACTTGGCCCAGTTCAGCGAGAACACGCCACTGTACCC AATCTGCCGCGCCTGGATGCGCAACAGCCCCTCTGTGCGCGAGCGTGAATGCTCTCCCAGCTCACCCCTG CCCCCGCTGCCTGAGGATGAGGAGGGCTCAGAGGTAACCAACAGCAAGAGTCGTGATGTGTACAAGCTGC CGCCACCCACACCCCCGGGGCCACCCGGAGATGCCTGCAGATCCCGCATCCCATCTCCACTGCAGCCTGA GATGCAGGGCACCCCTGACGATGAGCCCTCTGAGCCCGAGCCCTCACCCTCCACACTCATCTATCGCAAC ATGCAGCGCTGGAAACGCATCCGCCAGAGGTGGAAGGAGGCCTCTCATCGGAACCAGCTTCGTTACTCAG AAAGCATGAAGATCCTACGAGAGATGTACGAACGACAGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009071 unedited TTGTATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTGGACACAACCAAAG GCGGAGGACCCGTGGCCCACGAAGCTCATCTTTGAACTGTCCCCGCCTTCTCCCGCCTTG ACTTGTGACCCTAGGCCCTTTGGGGCGCCTCTGACCCAGCTAGCCAGATCCCGGACCCAA ACCATGTTCCCTGTGAAGGTGAAAGTGGAGAAATCAGAGCTGGAGATGGCCAAAGCCCGG AACCAACTGGATGCTGTCTTGCAGTGTCTGCTGGAGAAGAGTCACATGGACAGGGAGCGT CTGGATGAGGAAGCTGGGAAAACACCCTCAGACACCCACAATAAGGACTGCTCCATCGCA GCCACTGGCAAAAGGCCATCTGCCCGCTTCCCCCACCAGCGGAGGAAGAAGAGGAGGGAG ATGGATGATGGGCTGGCTGAGGGAGGGCCGCAGCGATCCAACACATATGTGATCAAGCTG TTCGACCGGAGCGTGGACTTGGCCCAGTTCAGCGAGAACACGCCACTGTACCCAATCTGC CGCGCCTGGATGCGCAACAGCCCCTCTGTGCGCGAGCGTGAATGCTCTCCCAGCTCACCC CTGCCCCCGCTGCCTGAGGATGANGAGGGCTCAGAGGTAACCAACAGNCAGAGTCGTGAT GTGTACAAGCTGCCGCCACCCACACCCCCGGGGCCACCCGGAGATGCCTGCAGATCCCGC ATCCCATCTCCACTGCAGCCTGAGATGCANGGCACCCCTGACGATGAGCCCTCTGAGCCC GAGCCCTTACCCTTCACACTTATNTATCGCANCATGCAGCGCTGGAAACGCCATCGCCAG AGTGGAAAGGAGCCTCTCATCGGAACCAGCTTCGTTCTCAAAAGCTGAAGATCTCGAGAG AGTACGAC
Restriction Sites	NotI-NotI
ACCN	BC009071
Insert Size	7000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009071.1, AAH09071.1
RefSeq Size	898 bp
RefSeq ORF	741 bp
Locus ID	55957
Gene Summary	This gene encodes a protein expressed in the eye. [provided by RefSeq, Jul 2008]

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