RASAL3 (AL832868) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832868, the custom clone sequence may differ by one or more nucleotides GTCCCGGTCCGGCGTCCTGCCCGCCGCCGCCAATCTGCGGGGCCCTGGCCGCGACCCAAAGGCTCCCTGA GCATGGGACCAGCGCCCCGCGCCCGGCCTTGGACCCGGGACTCCGCCTCGCTGCCTCGGAAGCCGTCGGT ACCCTGGCAGCGCCAAATGGACCAGCCGCAAGACCGAAACCAGGCACTGGGCACGCACCGACCTGTGAAC AAGTTGGCAGAGCTGCAGTGCGAGGTGGCCGCTCTGCGTGAGGAGCAGAAAGTGCTGTCCCGCCTCGTGG AGTCGCTGAGCACCCAAATCCGGGCCTTGACGGAGCAGCAGGAGCAGCTGCGGGGCCAGCTGCAGGATCT GGACTCCAGGCTCCGTGCTGGGAGCTCAGAGTTTGATTCAGAGCACAACCTAACAAGCAATGAAGGGCAC AGTCTGAAAAACCTGGAGCACCGCCTAAATGAGATGGAGAGAACTCAGGCTCAGCTGAGGGATGCTGTCC AGAGCCTGCAGCTTTCTCCAAGGACGCGGGGGTCTTGGAGTCAACCCCAGCCCCTCAAAGCACCCTGCCT CAATGGAGACACCACCTGAGCTGCCCATCCTGCCTCATCACACGTGGTCTGGGAGCAGAGAGATAGCCAT CTTAGGGGGGGTGTCTGACTTTGCCTTAGCCCTACTTGGCCTACAGTGGGGAGTGGAGCTGCTGGTCCCA ACCACTCTGGCAGTATGAAGTTGCCCAGTAAAATCTTGATTTCAGTGAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832868 unedited GGATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACCAGCCCAAGCACAC CCCTCTCATCTCCAANAGCCAGTCTCTGCGCAGCGTTCGCCGCTCAGAGAGTTGGGCCCG GCCACGGCCGGACGAAGAGCGGCCCCTGCGGCGGCCCCGGCCGGTGCAGCGCACGCAGAG TGTCCCGGTCCGGCGTCCTGCCCGCCGCCGCCAATCTGCGGGGCCCTGGCCGCGACCCAA AGGCTCCCTGAGCATGGGACCAGCGCCCCGCGCCCGGCCTTGGACCCGGGACTCCGCCTC GCTGCCTCGGAAGCCGTCGGTACCCTGGCAGCGCCAAATGGACCAGCCGCAAGACCGAAA CCAGGCACTGGGCACGCACCGACCTGTGAACAAGTTGGCAGAGCTGCAGTGCGAGGTGGC CGCTCTGCGTGAGGAGCAGAAAGTGCTGTCCCGCCTCGTGGAGTCGCTGAGCACCCAAAT CCGGGCCTTGACGGAGCAGCAGGAGCAGCTGCGGGGCCAGCTGCAGGATCTGGACTCCAG GCTCCGTGCTGGGTGAGCCCAGCCCTCTCCATTAGCTCCGCCCCCAGGTGGGCCCGATCC AATTAGATCTGACCAGGCCCTCCCCCCCCCCCAAACCACGCCTAGCACGGCCAAGCCCCA CCCTGGCCCCGCCCCAATAAGTCTGTCCAGGCCCTGCCTCTTGGCAAGCCCCGCCCCCAG GTGGACCCACCCAACGAAGGTCGGCCTAGGACCCTGGCAGATTCTCTCATCCAGGCCCCA CCCCTCCATCAGCCCCTCCCAACCTGGCTGGGCCGGGCACTGAGATATCTCAGCCTGGGC ACTCCCTCCAGCTCAGTCACGACCCCACCCAGGACTGGCCAAACCCCACTNCAAGGGATG ATTGATAGGTCCTGTCATTCCACCATTNCCGTCTCTGGTCCGACCCTTCTCAAGCATATT AGCCCCGCCCCTCATCTGGACAGCTCGCCTCCATCCAAGCTCGCCCC
Restriction Sites	NotI-NotI
ACCN	AL832868
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL832868.1
RefSeq Size	780 bp
RefSeq ORF	780 bp
Locus ID	64926
Gene Summary	This gene belongs to the Ras GTPase-activating proteins (RasGAP) family and encodes a protein with pleckstrin homology (PH), C2, and Ras GTPase-activation protein (RasGAP) domains. This protein is localized near or at the plasma membrane when expressed exogenously. Reduced expression of this gene in some cell lines resulted in increased levels of the active form of Ras (Ras-GTP), suggesting that this gene may play a role in negatively regulating the Ras signaling pathway. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

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