SPSB3 (AF403028) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C16orf31; SSB3
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF403028, the custom clone sequence may differ by one or more nucleotides ATGGCCAGACGCCCCCGGAACAGCAGGGCCTGGCACTTCGTCCTGAGTGCAGCCCGCCGAGACGCAGATG CCCGGGCCGTGGCTCTAGCAGGCTCCACTAACTGGGGCTACGACTCTGATGGGCAGCACAGCGACTCGGA CTCCGACCCCGAGTACTCCACGCTGCCGCCATCCATCCCCAGTGCGGTGCCCGTGACCGGCGAGTCCTTC TGTGACTGTGCTGGGCAGAGCGAGGCCTCCTTCTGTAGCAGCCTGCACTCGGCCCACCGGGGCAGGGACT GCCGCTGCGGAGAGGAAGACGAGTATTTCGACTGGGTCTGGGATGACTTAAATAAGTCATCAGCCACCCT GCTGAGCTGTGACAACCGTAAGGTCAGCTTCCACATGGAGTACAGCTGCGGCACAGCGGCCATCCGGGGC ACCAAGGAGCTGGGGGAGGGCCAGCACTTCTGGGAGATCAAGATGACCTCTCCCGTCTACGGCACCGACA TGATGGTGGGCATCGGGACGTCGGATGTGGACCTGGACAAATACCGCCACACGTTCTGCAGCCTGCTGGG CAGGGATGAGGACAGCTGGGGCCTCTCCTACACGGGCCTCCTCCACCACAAGGGCGACAAGACCAGCTTC TCGTCGCGGTTCGGCCAGGGCTCCATCATTGGCGTGCACCTGGACACCTGGCACGGCACACTCACCTTTT TCAAGAACAGGAAGTGTATAGGTGTGGCAGCCACCAAGCTGCAGAACAAGAGATTCTACCCGATGGTGTG CTCCACGGCGGCCCGGAGCAGCATGAAGGTCACCCGCTCCTGTGCCAGCGCCACTTCCCTCCAGTACCTG TGCTGCCACCGCCTGCGCCAGCTGCGGCCAGACTCGGGAGACACGCTGGAGGGTCTGCCGCTGCCGCCGG GCCTCAAGCAGGTGCTACACAACAAGCTGGGCTGGGTCCTGAGCATGAGTTGCAGCCGCCGCAAGGCTCC AGTGTCCGATCCCCAGGCAGCGACCTCCGCCCACCCCAGCAGTCGCGAGCCTCGGCCCTGCCAGAGGAAG CGCTGCCGCCGGACCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF403028 unedited GTTAGTCTTGTAACAGATTCATATAGGCGGCCGCCGAATTCGCACGAGGGCCGGCGGAGC GCGCAGCGGGGGCTGCAGATTCTTTCCACCATGGCCAGACGCCCCCGGAACAGCAGGGCC TGGCACTTCGTCCTGAGTGCAGCCCGCCGAGACGCAGATGCCCGGGCCGTGGCTCTAGCA GGCTCCACTAACTGGGGCTACGACTCTGATGGGCAGCACAGCGACTCGGACTCCGACCCC GAGTACTCCACGCTGCCGCCATCCATCCCCAGTGCGGTGCCCGTGACCGGCGAGTCCTTC TGTGACTGTGCTGGGCAGAGCGAGGCCTCCTTCTGTAGCAGCCTGCACTCGGCCCACCGG GGCAGGGACTGCCGCTGCGGAGAGGAAGACGAGTGTGAGCGTGGGGCCCAGCCCCTGCTG GGACTGCACTGCGGGCGGGCCTGGGGGTGGGCAGGGCAGAGCCCCATGGAGCTCTCAGGG CTGCCCCAGCCTGGGCCTCTCTTGCAGATTTCGACTGGGTCTGGGATGACTTAAATAAGT CATCAGCCACCCTGCTGAGCTGTGACAACCGTAAGGTCAGCTTCCACATGGAGTACAGCT GCGGCACAGCGGCCATCCGGGGCACCAGGGAGCTGGGGGGAGGGCCAGCACTTCTGGGAG ATCAAGATGACCTCTCCCGTCTACGGCACCGACATGATGGTGGGCATCGGGACGTCGGAT GTGGACCTGGACAAATACCGCCACACGTTCTGCAGCCTGCTGGGCAGGNATGANGACAGC TGGGGCCTCTCCTACACGGGCCTCCTCCACCACAAGGGCGANCAGACCAGCTTCTCCGTC GCGGTTCGGCCAGGGCTCCATCATTGGGCGTGCACCTGGACACCTGGCACGGGACACTCA CCCTTTTTCAGANCAGGGAGTGTTATAGTTGTGGCAGCCACCAAGCTGCAGACCAGAGAT TCTACCCGATGTGA
Restriction Sites	NotI-NotI
ACCN	AF403028
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF403028.1, AAL57347.1
RefSeq Size	1068 bp
RefSeq ORF	1068 bp
Locus ID	90864
Domains	SPRY
Protein Families	Druggable Genome
Gene Summary	May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.[UniProtKB/Swiss-Prot Function]

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