GPSM1 (BC017353) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZP727I051
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC017353, the custom clone sequence may differ by one or more nucleotides ATGGAGGCGTCCTGCCTAGAGCTGGCGCTGGAGGGCGAGCGTCTGTGCAAGGCGGGCGACTTCAAGACAG GCGTGGCCTTCTTTGAGGCTGCTGTGCAGGTGGGCACCGAGGACCTGAAGACACTGAGTGCCATCTACAG CCAGCTGGGCAACGCCTACTTCTACCTGAAGGAGCACGGCCGGGCGCTGGAATACCACAAGCATGACCTC CTGCTGGCGCGGACCATCGGTGACCGCATGGGGGAGGCCAAGGCCAGTGGAAACCTGGGAAACACACTCA AGGTCCTGGGGCGCTTCGACGAGGCTGCCGTCTGCTGCCAGCGGCATCTGAGCATCGCCCAAGAGCAGGG AGACAAGGTTGGGGAGGCGAGGGCCCTCTACAACATCGGGAACGTGTACCACGCCAAAGGCAAGCAACTG TCCTGGAACGCCGCAAACGCCACGCAGGACCCCGGGCACCTGCCGCCCGATGTCCGAGAGACCCTGTGCA AGGCCTCCGAGTTCTACGAGAGGAACCTGTCCCTGGTGAAGGAGCTGGGCGACCGTGCGGCGCAGGGCAG GGCCTACGGCAACCTGGGCAACACCCACTATTTGTTGGGGAACTTCACAGAGGCCACGACCTTCCACAAG GAGCGCCTGGCCATTGCTAAGGAGTTTGGAGACAAGGCAGCCGAGAGGAGGGCCTACAGCAACCTGGGGA ACGCCCACGTCTTCCTGGGGCGCTTTGACGTGGCCGCCGAGTACTACAAGAAGACGCTGCAACTGTCTCG GCAGCTCAGGGACCAGGCAGTGGAGGCGCAGGCCTGCTACAGTCTGGGCAACACCTACACGCTGCTGCAG GACTACGAGCGCGCGGCCGAGTACCACCTGCGGCACCTGCTCATTGCCCAGGAGCTGGCCGACAGAGTGG GCGAGGGCCGGGCGTGCTGGAGCCTGGGAAATGCCTACGTGTCCATGGGGCGCCCAGCGCAGGCCCTGAC CTTCGCCAAGAAGCACCTGCAGATCTCCCAGGAGATCGGGGACCGCCATGGGGAGCTCACGGCCCGCATG AACGTGGCGCAGCTGCAGCTGGTGCTCGGCCGCCTGACCAGCCCGGCAGCCTCAGAGAAGCCTGACCTGG CCGGCTATGAGGCCCAGGGTGAGTTCCAGGGTTGTGGGGGGGTCTTGCTCCCCACAGGCACGGACCGCAT CAGGAGCTGCGGAGGGGTGGGATCGAGGCCAGGCCAGCATGGCGGAGGTGGCAGCCGCCAGAAAATGGCG CCTACAAGCCAGTTCTTCTTGGCCTCAGGGACAGCACAGGCCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017353 unedited TTTCCCCGCCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG AATTCGGCACGAGGCAGCAGGGCGGACAGCAGGGAGGACAGCAGGGCGGGCAGGGGGCGG ACGGCCACGGCGCGGGGGGCGCTCCCGGCTCCCGCTCCCGCGTCCCCGACCCATGGCGGG CCCGGCCCCGCCCGCGGCCGACGAGCTCCCGGGCCCGGCCGCCAGGCGCCTCTACTCCAG GATGGAGGCGTCCTGCCTAGAGCTGGCGCTGGAGGGCGAGCGTCTGTGCAAGGCGGGCGA CTTCAAGACAGGCGTGGCCTTCTTTGAGGCTGCTGTGCAGGTGGGCACCGAGGACCTGAA GACACTGAGTGCCATCTACGGCCAGCTGGGCAACGCCTACTTCTACCTGAAGGAGCACGG CCGGGCGCTGGAATACCACAAGCATGACCTCCTGCTGGCGCGGACCATCGGTGACCGCAT GGGGGAGGCCAAGGCCAGTGGAAACCTGGGAAACACACTCAAGGTCCTGGGGCGCTTCGA CGAGGCTGCCGTCTGCTGTCAGCGGCATCTTGACATCGCCCAAGAGCAGGGAGACAAGGT TGGGGAGGCGAGGGCCCTCTACAACATGGGGAACGTGTCCCACGCCAAGGGCAGCAACTG GCCTGGAACGCCGAAACGCCCCGCAGACCCCTGGCACCTCCGCCCGAGTCCCAAAACCTT GTGCAGGCCTCCAGTTTACCAGAGGACCTGTCCCTGTGAAGGCACTGGCCACCGTCGCGC CAGGTAGGCCATGGCACCTGGCACACCCCTTTTGTTGGGACTCCAAAGCCTCACCTCCCA GGCCCCTGCCTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC017353 unedited TCTANNGATCGATTTTTTTTTTTTTTTTTTATAAAAACATTTAATTATTAAAAATAAGAT AAGAAAAAGAAACTCCGGCAGCCCAGCACCACATCCAAGCCTGGTGTTCCCAGCCTGGTC CTTAGCAGGCCTGGGGGGCACCAGGTACGTTCTGGGAGGCAGGCATCCCTTCTCGGGGGC TGGCAAACTCAAGACCTTTGGGAGTGGGGGACACATGGGTGGGGCAGCTGCTCCCCCCAA GCCTGCCCCTCACCCACCTGCCAGCCACCTCAGGAAGTCGGCCCAACCTGGCACCACCCC CCTGGCACCTATTAGTCTTTTCGCTTTGCAGGGGACGTTCTGTCCTGTGTCTGCAGCACT GCTGACCGGCATGGGACGACCAGCGGTCCATGAATGAGGTCACCACGGTGGCCTCGCATG GCCTTGGGCCTTGCCTGATTACTGCCCCAGGCACATAGGGGTCCCACAGCCCAGCCCAGC CCAGCCCAGCCCTCTGGCTGATGCCCTCTCGCCTCCCAGCAGCCCTCTCCTACTTGGGTC TGAGGACAGAAACCCTCCCTGCACCGAGGGCTCCCAGGGGAAGTGGGGGTGGAAGCCCTT TAACAACCACTGGGGCAAAAAGGGAACACCTCCTGTTCTTTATTCAGCAACCCTGGAGGG CCCCTTCTGATTCCGGTGGGAAAGGGTTAAGGTGAACCCCAAGCCCGGGCTGTCCCCCTG GGCTAATAACCGGCCAAGTCAAGGTTTTTTAAGGTTTCCGGGTTGGTCATGGCGGGCGAA AACCAACTTTGAGGTGGGCCCCCTTTAATGTGTGGCCCGGAGGCTTCCCATGGGGG
Restriction Sites	NotI-NotI
ACCN	BC017353
Insert Size	2200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017353.1, AAH17353.1
RefSeq Size	2296 bp
RefSeq ORF	1305 bp
Locus ID	26086
Domains	TPR
Gene Summary	G-protein signaling modulators (GPSMs) play diverse functional roles through their interaction with G-protein subunits. This gene encodes a receptor-independent activator of G protein signaling, which is one of several factors that influence the basal activity of G-protein signaling systems. The protein contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

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