PLEKHM2 (BC008002) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KIAA0842; novel RUN and PH domain-containing protein; pleckstrin homology domain containing, family M (with RUN domain) member 2; RP11-169K16.1; SifA (Salmonella-induced filaments A) and kinesin-interacting protein; SKIP
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC008002, the custom clone sequence may differ by one or more nucleotides GATGGTCCATTCCTCGGAGTTCAGAGTAGACAACAATCACCTGCTCCTGCTCATGATCCACGTGTTCCGA GAAAACGAAGAGCAGCTGTTCAAAATGATCCGGATGAGCACCGGGCACATGGAGGGCAACCTGCAGCTGC TGTACGTGCTGCTCACAGACTGCTATGTCTACCTGCTCCGGAAAGGGGCCACAGAGAAGCCATACCTGGT GGAAGAGGCCGTTTCTTACAATGAACTTGACTATGTGTCGGTTGGCCTTGACCAGCAGACGGTGAAGCTG GTGTGCACCAACCGCAGGAAGCAGTTTCTGCTGGACACGGCTGATGTGGCGCTGGCTGAGTTCTTTTTGG CTTCTTTGAAGTCAGCCATGATCAAAGGCTGTCGAGAACCTCCCTACCCCAGCATCCTGACGGATGCCAC CATGGAGAAGCTGGCACTGGCCAAATTTGTGGCCCAAGAATCGAAGTGTGAGGCATCTGCTGTCACCGTG CGCTTCTACGGCCTTGTGCACTGGGAGGACCCCACAGACGAGTCCCTGGGCCCCACGCCCTGCCACTGCT CACCCCCCGAGGGCACCATCACCAAAGAAGGCATGCTGCACTACAAGGCGGGCACCTCCTACCTGGGCAA GGAACACTGGAAGACGTGCTTCGTGGTGCTCAGCAACGGGATCCTCTACCAGTACCCGGACCGCACCGAC GTCATCCCTCTGCTCTCGGTGAACATGGGGGGGGAGCAGTGCGGTGGCTGCCGGAGAGCCAACACCACGG ATCGGCCCCACGCCTTCCAGGTCATTCTCTCCGACCGGCCCTGCCTGGAGCTAAGTGCCGAGAGCGAGGC CGAGATGGCCGAGTGGATGCAGCATCTCTGCCAGGCTGTGTCCAAAGGGGTCATCCCCCAGGGCGTAGCT CCCAGCCCCTGCATACCCTGCTGCCTGGTCCTCACGGATGACCGCCTCTTTACGTGCCATGAGGATTGCC AGACCAGCTTCTTCCGCTCTTTGGGCACAGCCAAGCTGGGCGACATCAGCGCCGTCTCCACCGAGCCGGG CAAGGAGTACTGCGTCTTGGAGTTCTCCCAGGACAGCCAGCAGCTCCTCCCGCCCTGGGTCATCTACCTG AGCTGCACTTCTGAACTGGACCGATTGCTGTCTGCACTGAACTCTGGGTGGAAAACCATCTATCAGGTGG ACCTCCCCCACACGGCGATCCAGGAAGCCTCCAACAAGAAGAAATTCGAGGATGCCTTGAGCCTCATCCA CAGCGCCTGGCAGCGGAGCGACAGTCTCTGCCGCGGCCGAGCCTCCCGAGACCCCTGGTGCTGAGGCAGA GCTGGTTGGCGTCCCTGGTGGGCAGGAAAGGAAGGCACGCCAGCCGGCAGGCACACTGTCACGGCTGTTG TCATGCTGTCGGGAGCCTACAGTCCACCCCTGCCCTGGGCGGCAGAACCACCGAGTGTGGCTTAAGACAG GGTCCCTCCACTCCAGGGATCCAGATCAGGTGCCCGGCACCCCTGGGCATCCTGCCCGACAGGTAGCGAA TGGAGGTCGCTGGGGGCAGAGGGTCCGAGCCCTGTGGGCTCTGCGGATGCACGCCCTCCTCCCGGGCCTC CGCCTCAGTCTGCAGAATTTCTGCCGAGTGGCACCGAGAACACCATCCATCTAAGGACGAACAAAAGAAC CAGGAGGGCGGGACCCCCCTCTTCCTCTCCTGGGTTGGGGGCTGGGGCCCTGAGTGCCCAGCCATCCTTG TTCGTGTTTGAACACTCTCCTGGCCACGTGGGGAAGCGGGAACACGGGGTGTCTGCGCATGTTTCCTCCT CCTAGCTCCATCACTGCGCACACAGCTGCCTGCCTCGCCAGATGCAGGGGGGCGGGCAGCCCTCCCTGGC TGCCAGGAGGCTCTGCATGCCCACAGTCCTGCCCTGCCTGTCCCCTCAACCCGGCAGTGCCTGTAGCACC GAGGAGCAAAGGGGGTGGATGGGGGGCTTGGAGAAGGGCGGAGCCCACCAGCCTGGCATCCATGTTGACA TCTTCTGACTGTCCCCTGCTTGGCTGGAGCCAGGCCCTTCCCTAGAGTTTCGTCAAGAGCCTCCTGGGGA AGGGGTCAGGTGGTTTGGGTTTTGTTTTTTAAAATAAAATAGACATGTTATATTGCAAAAAAAAAAAAAA AAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008002 unedited CACATTTTGTATACGACTACTATAGGCGGCCGCGAAATTCGCACGAGGGGACCCAGGAGG TTCTCTGCCAGCTCAAGCGAGACCAGCCCAGCCCGTGTCTGAGTAGCGCTGAGGATTCTG GGGTGGATGAGGGACAGGGGAGCCCTTCGGAGATGGTCCATTCCTCGGAGTTCAGAGTAG ACAACAATCACCTGCTCCTGCTCATGATCCACGTGTTCCGAGAAAACGAAGAGCAGCTGT TCAAAATGATCCGGATGAGCACCGGGCACATGGAGGGCAACCTGCAGCTGCTGTACGTGC TGCTCACAGACTGCTATGTCTACCTGCTCCGGAAAGGGGCCACAGAGAAGCCATACCTGG TGGAAGAGGCCGTTTCTTACAATGAACTTGACTATGTGTCGGTTGGCCTTGACCAGCAGA CGGTGAAGCTGGTGTGCACCAACCGCAGGAAGCAGTTTCTGCTGGACACGGCTGATGTGG CGCTGGCTGAGTTCTTTTTGGCTTCTTTGAAGTCAGCCATGATCAAAGGCTGTCGAGAAC CTCCCTACCCCAGCATCCTGACGGATGCCACCATGGAGAAGCTGGCACTGGCCCAAATTG TGGCCCAAGAATCGAAGTGTGAGGCATCTGCTGTCACCGTGCGCTTCTACCGNCTTGTGC ACTGGGAGGACCCCACAGACGAGTCCCTGGGCCCCACGCCCTGCCACTGCTCACCCCCCC GAGGGCACCATCACCAAAGAAGGCATGCTGCACTACAAAGCGGGNCACCTTCTACCCTGG GCAAGGGACACTGNGAGACGTGCTTCGTGGTGCTCAGCAACGGGATCCTTTACCAGTACC CGGACGCACCGACGTNATCCTCTGCTCTCGTGAAAATGGGGGGGGAGCATGCGGGGGCTG CCGGAAACAACACCACGGACGGCCCACCCTCCAGTATTCTTTCCACGGCCTGTCTGGACT AATGCCAAAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC008002 unedited TTTATGGTACCGCGGCCGCATTCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTGGCAAAATAACATGTCTATTTTATTTTAAAAAAAAAA ACCCAAACCACCTGACCCCTTTCCCAAGAAGGTTTTGACCAAACTTTTGGGAAGGGCCTG GCTCCCGCCCAACAGGGGACAATCAAAAAAAGTTTACTTTGATGCCCAGCTGGGGGGCTC CCCCCTTTTTCAAACCCCCCATCCACCCCCTTTTGTTCTCGGGGCTACAAGCACTGCCCG GTTGAGGGGACAAGCCAGGCAAGACTGTGGGCATTCCAAACCTCCTGGCAACCAAGGAGG GCTGCCCCCCCCCCTGCATTTTGGGAAGCAAGCAACTTTGTGCCCCCTGATGGAGCTTGG AAGAAGAAACATGCCCCAACACCCCGTGTTTCCCCTTTCCCACGTGGCCCGGAAAGTGTT TAAACACCAACAAAGGTGGGTGGGCACTCAAGGGCCCCGCCCCCCACCCCAGAAAAGAAA AAGGGGGTCCCCCCCCCCCGGGTCTTTTGTTCCTCCCTAAAAGGAAGGGGGTCTCGGGGC CACTCCGCAAAAATTTTTCAAACTTAAGCCGAAGCCCCGGAAGAAGGCGTGCATTCCCAG AACCCACAAGGGTTGGGCCCTCTGGCCCCAACGAACTTCATTTGGCTAACTGTTTGGCAA GAAGCCCCAGGGTGCCCGGGCACTGATTTTGATTCCTGGAGTGGAGGACCCTGTCTTAAA CCACACTCGTGGGTCTGCCCCCCAAGCAAGGTTGGACTGTAGCTTCCCAACACTGAAACC ACCCTGAAATGTGCTGCCGCCTGGGGTGCCTTCTTTCTGCCCCCAGGGACCAACAATTTT GCTTTAACCAGGGTTTTGGAGGCTCCCCCGCAAAAACTTCC
Restriction Sites	NotI-NotI
ACCN	BC008002
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008002.1, AAH08002.1
RefSeq Size	2176 bp
RefSeq ORF	2176 bp
Locus ID	23207
Domains	PH
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein that binds the plus-end directed microtubule motor protein kinesin, together with the lysosomal GTPase Arl8, and is required for lysosomes to distribute away from the microtubule-organizing center. The encoded protein belongs to the multisubunit BLOC-one-related complex that regulates lysosome positioning. It binds a Salmonella effector protein called Salmonella induced filament A and is a critical host determinant in Salmonella pathogenesis. It has a domain architecture consisting of an N-terminal RPIP8, UNC-14, and NESCA (RUN) domain that binds kinesin-1 as well as the lysosomal GTPase Arl8, and a C-terminal pleckstrin homology domain that binds the Salmonella induced filament A effector protein. Naturally occurring mutations in this gene lead to abnormal localization of lysosomes, impaired autophagy flux and are associated with recessive dilated cardiomyopathy and left ventricular noncompaction. [provided by RefSeq, Feb 2017]

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