MOV10 (AL832805) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	fSAP113; gb110
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832805, the custom clone sequence may differ by one or more nucleotides GGCGTAATGGGCAAAGATGAGCGTGAAGGCAACAGCCCATCCTTCTTCAACCCTGAAGAGGCTGCCACAG TGACTTCCTACCTGAAGCTGCTCCTGGCCCCCTCCTCCAAGAAGGGCAAAGCCCGCCTGAGCCCTCGAAG TGTGGGCGTCATCTCCCCGTACCGGAAACAGGTGGAGAAAATCCGTTACTGCATCACCAAACTTGACAGG GAGCTTCGAGGACTGGATGACATCAAGGACTTGAAGAACACTTTGCAGGCCCCAGCCCTCAGCAGGATTC AACCTTCAGGTTTCAGCTTCCACAGCCTACCTCCCATCCCCTTTCCCTGGGCCGTGTCGCAGCACTGGTT CACTCACTGATCTACAATTGCCCAGTGACCTCTCTGTACCCTCCTTGGAAGGCAAGCTCCCTGGGGTAGG GCACCCCTTTGACACCCCTGGCTGGTCTCAGGCCCTGCCTCTTTCCCCACTAGGTGGGTTCAGTAGAAGA ATTCCAAGGCCAAGAACGAAGCGTCATCCTCATCTCCACCGTGCGAAGCAGCCAGAGCTTTGTGCAGCTG GATCTGGACTTTAATCTGGGTTTCCTTAAGAACCCCAAGAGGTTCAATGTAGCTGTGACCCGGGCCAAGG CCCTGCTCATCATCGTGGGGAACCCCCTTCTCCTGGGCCATGACCCTGACTGGAAAGTGTGAGCATTCCC ACCCCATTCTCCCTCTTAGTGGCCACAGCCCCTGCCTAGGGCAGGTGGATCTTTTTTAAGCGCTTGCTTA TCGCTCAGTTAATCCTCAGATGTGTCCATTTTCACAGCATCACTATTGTATTTATAAGTTAAATGACATC CAGAGGTTGAGCAAGTACAGCTCAGATGGGACAGGACCGTGGCTTAGCCTCCAGTCTCTGCTGGCACTCC TCTGTACCCCACAGATTCCTGGAGTTCTGTAAAGAAAACGGAGGGTATACCGGGTGTCCCTTCCCTGCCA AACTGGACCTGCAACAGGGACAGAATTTACTGCAAGGTCTGAGCAAGCTCAGCCCCTCTACCTCAGGGCC CCACAGTCATGACTACCTCCCCCAGGAGCGGGAGGGTGAAGGGGGCCTGTCTCTGCAAGTGGAGCCAGAG TGGAGGAATGAGCTCTGAAGACACAGCACCCAGCCTTCTCGCACCAGCCAAGCCTTAACTGCCTGCCTGA CCCTGAACCAGAACCCAGCTGAACTGCCCCTCCAAGGGACAGGAAGGCTGGGGGAGGGAGTTTACAACCC AAGCCATTCCACCCCCTCCCCTGCTGGGGAGAATGACACATCAAGCTGCTAACAATTGGGGGAAGGGGAA GGAAGAAAACTCTGAAAACAAAATCTTGTTCTATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832805 unedited TAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGCAACTACAGG TCTCATCCCACCATCCTGGACATTCCTAACCAGCTCTATTATGAAGGGGAGCTGCAGGCC TGTGCTGATGTCGTGGATCGAGAACGCTTCTGCCGCTGGGCGGGCCTACCTCGACAGGGC TTTCCCATCATCTTTCACGGCGTAATGGGCAAAGATGAGCGTGAAGGCAACAGCCCATCC TTCTTCAACCCTGAAGAGGCTGCCACAGTGACTTCCTACCTGAAGCTGCTCCTGGCCCCC TCCTCCAAGAAGGGCAAAGCTCGCCTGAGCCCTCGAAGTGTGGGCGTCATCTCCCCGTAC CGGAAACAGGTCAGGTCCTCAGTTACCAGCAAGGGTGGGGCCCCTCCCCCAGATGGTACC TCCTTAATATCCAGACCACTAGGCAGAGGCTCCAGGAGCTTAGGCCTCTGCTGGCTCCGT ATCTCAGAAGAGCACCAAGGTCAGCTGCCGCCTCCCTTTGTTCCCCAGCTCCCTGGCCTC CTGCCAGGCTCCCTCTTGCATTAGGTTAATGGCACGAGAGAAAGGCACCTGTCCCCTCCT TCCAGGTGGAGAAAATCCGTTACTGCATCACCAAACTTGACAGGGAGCTTCGAGGACTGG ATGACATCAANGACTTGAAGGTGACATGCTGTTCCACAGTCACTCCCTGCCTTCCGTGTG CCCCCACTTGCCCACTTNCAGAGACTTNCTCAAGCTTCCACTCCAGCCCACGTNCCCGTN CCACCCCTGCTGNCTTGAATAGAGCTCGAGCTCTCCCTGAGCCTCTCACTCCTGNAGATN CCAACTAANGGTCTGGNATGGGATCACGAAGCCTGCATGTTTAACAACACCTCCTGTCAT TCTTAAACTGTAGCACGGTTGAAGCCTCTGCCCTAAAGATAAAGTCAAATNNCGTGCCTG TCATGTGATGCTGCCCCCCCCACCCCAGTAGCTCTCTCTAAGGTTCTGTCTAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL832805 unedited TCTANGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTCAAAATT TTCTTCCTTCCCCTTCCCCCAATATGTTAACAACTTGATGTGTCATTCTCCCCAACAAGG GAAGGGGTGGAAAGGCTTGGGTTGTAAACTCCCTCCCCCAACCTTCCTGTCCCTTGGAAG GGCAATTCAACTGGGTTCTGGTTCAAGGTCAAGCAGGCAGTTAAAGCTTGGCTGGTGCCA AAAAGCTGGGTGCTGTGTCTTCAAAACTCATTCCTCCACTCTGGCTCCACTTGCAAAAAC AGGCCCCCTTCACCCTCCCGCTCCTGGGGGAAGTAATCATGGCTGTGGGGCCCTGAAGTA AAAGGGCTGAACTTGCTCAAACCTTGCAATAAATTCTGTCCCTGTTGCAAGTCCCATTTG GCAGGGAAAGGACACCCCGTATACCCTCCGTTTTCTTTACAGAACTCCAAGAATACTTTC CAGTCAAGGTCATGGCCCAAGAAAAAGGGGTTCCCCACCATGATGAACAAGGCCTTGGCC CCGGTCACAACTTCCTTGAACCTCTGGAAAGAAGAAATGGTGTGGTCATGAAAGGGAAAA ACCCCCAAAATGGAAGAATTCCTGCCACCCCGAACAAACCTCAAACCTTGGGGTTCTTAA GGAAACCCAGATTAAAGTCCAAATTCAGCTGCACCAAACCTTGGCTGCTTTCCACGGTGG GAGATTAGGATGACCCCTTCCTTCCTTGGCCCTTGAAATTTTTCTACTGAACCCACCTAA TGGGGAAAAAAACCAGGGCCCTGAAACCACCCAAGGGTGTCTAAAGGGGTGCCCCTACCC CAGGAAGCTTTGCCCTTCCAGGAAGGTACAC
Restriction Sites	NotI-NotI
ACCN	AL832805
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL832805.1
RefSeq Size	1399 bp
RefSeq ORF	1399 bp
Locus ID	4343
Gene Summary	5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs (PubMed:24726324). Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:16289642, PubMed:17507929, PubMed:22791714). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (PubMed:25464849). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1 (PubMed:30122351, PubMed:23093941). Facilitates LINE-1 uridylation by TUT4 and TUT7 (PubMed:30122351). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (By similarity). May function as a messenger ribonucleoprotein (mRNP) clearance factor (PubMed:24726324).[UniProtKB/Swiss-Prot Function]

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