FANCI (BC004277) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Fanconi anemia, complementation group I; FLJ10719; KIAA1794
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC004277, the custom clone sequence may differ by one or more nucleotides ATGTTCAAGGATGTCCCTCTGACTGCAGAAGAGGTGGAATTTGTGGTGGAAAAAGCATTGAGCATGTTCT CCAAGATGAATCTTCAAGAAATACCACCTTTGGTCTATCAGCTTCTGGTTCTCTCCTCCAAGGGAAGCAG AAAGAGTGTTTTGGAAGGAATCATAGCCTTCTTCAGTGCACTAGATAAGCAGCACAATGAGGAACAGAGT GGTGACGAGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC004277 unedited NNAAAAGTTCAGGATATTTGTNAATACGCACTCCACTCTTAGGGGCGGGCCGCGCAATTC CGCACGAGGACGGCGTTGGGTTGAGCGGGCTTTTTGGGAAGTTGGGGGGGAGAGTCTCCC TCTGTTGCCAGGCTGGAGTGCAGTGGCGCGATCTCCGCTCACTGCAACCTCCGCCTCCTG AGTTCAAGTGATTCTCCTGCCTCAGCCTCACAAGTAGCTGGGACTACAGTTCTGTGATAT GAGCAACAATGGACCAGAGGAGGGTATCTCTANCANCAGAAAAAACAGCAGACAAACTGC AAGAATTTCTTCAAACCCTGAGAGAAGGTGATTTGACTAATCTCCTTCAGAATCAAGCAG TGAAAGGAAAAGTTGCTGGAGCACTCCTGAGAGCCATCTTCAAAGGTTCCCCCTGCGCTG AGGAAGCTGGAACACTTANGAGACGTAAGATATACACTTGTTGTGTCCAGTTGGTGGAAT CGGGGGATTTGCAGAAAGAAATAGCGTCTGAGATCATAGGATTACTGATGCTGAAGGCTC ACCATTTTCCAGGACCATTATTGGGTGAATTAGCCAATGAGTTTATTAGTGCTGTCANAG AAGGCGGCCTANTGAATGGAAAATCTTTGGAGTTACTACCTATCATTCTCACTGCGCTGG CTACCAAAAAGGAAAATCTGGCTTATGGAAAAGGTGTGCTGAGTGGGGAAGAATGTAAGA AACAGTTGATTAACACCCTGTGTTCTGGCANGTGGGATCCAGCATATGTAATCCAACTCA CCTCCATGTTCAAAGATGTCCCTCTGACTGCAGAAAAGGTGGGATTTGTGGGTGGGAAAA ACTTTGAGCATGTGCTCCAAGATGAATCTTCAAGAAATACAGCTTTGGGGCATCAGCTTC TGGTGCTC
Restriction Sites	NotI-NotI
ACCN	BC004277
Insert Size	4200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004277.1, AAH04277.1
RefSeq Size	1096 bp
RefSeq ORF	222 bp
Locus ID	55215
Gene Summary	The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group I. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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