F115A (TCAF1) (AL832802) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832802, the custom clone sequence may differ by one or more nucleotides TTCTGTTTCCAGGTGCCTGAGGAACATCTAAATCATCAGGTTATGTCTGAAATAAAGGGAGAGAGTATGC AGATTATTGCCTCTACGGGTCTGGATCTATTGATAAAGGAGATTTGGGCGGGTGGGGGGGCAAGAAGGAG TGCAGAGAAGAAAACCTGGGGAACGCATATCGCTGGGGAGGGACAGGCCATGAGGAGAACTAGGAGCAAG TGCTTTAGGGAAGAGGAATTTACCAGAAGCCACAGAGGGCCTGAGCATGAGGGCTCACACCTGTAGTCCC AGAGCTTTGGGAGGCCGAGGCAGGAGGATTGCTTGAGGCCACATGCTCTAGACTAGTCTGGGCAACATAG TGAGGCCCCATCTCTAGAAAAAGTTTTAAGTTAGTTGGGAGCAGTGGTGTGTGCCTATAGTCCTAGCTAC TTGGGAGGCTGAGGTGGAAAGATAACTTTAGCCAAGGAGTTCTAGCCTGCAGTGAACTATGATAGTACCA CTGCACTCCAGCCTGGGCAACAGAGCAAGGCTGTCTCTTAAAAAAAAAAAGGAAGCCACAGAGAGAGGCC AGGTAAGGACCCAGGGTCTCGTGGATTTTGCAATGGAGGTAAACACAGTGTGGGCTGATGCAATCAGTGT TTGCTGCCCTTGGGCGCTTCGCTTCACCTCTAAGGGGCTCTGCAACGTGATAGAGTTGGGCCAGTGAATT TGTCAGGTGTCTTCTGGCTGTTACTTTTGTCTGTGTGTGAACCTTTGTTTTAGGCCATTTCTGTAGTTAG TGAACAGAGCAGATAGAGTCCAGTCATTTTGCAGAAATGTTTCCGTTTGGATGATGAAACAAGCTTGATT GCTGAAGTTGGTGCACTCATCCCATTGGCATGGTGCTAGCCGAGACCTTTGAAATACCTGAGGACACCTC TTGCCTGTTTATAGAGGTTGCTTCTGTCTGCTGAATAGGGCTTTGTAATTAACCAGGAGGTGCATTTAGC GACACTGGCCATTTTTCTCTGCATACCAAGTCTACAACATCAAAGAAGAAATTTAGGATGAAAGTTCTTT AAAATCTTAGGCCAGGTGCTGCAGCTATTGCCATTGCAAGGACCTGCGGCAGTCCTGTTGGAAAATGTTT GTTCTGCATGTGTTCCATGCCAGTTGTTGGCAATAAGATTCTAAGGTTTTGGAACTAGACTACTGCAATG AAACAACTTTCTCTGTTAGGGAAGTGTCCTGGGCTGTCAAGAATTATCATCAAGAGCAGCAGGTTTTATA AGATACATGGACCTATTTGGCATTTACCAGCCCCTGCCGCTAGGATAGAGGGACAGGGCTGGGCCCCAAG TGTGGGCTTCCCGGAAGAGCCAAGCACCGGCTGCTCATATGGGATGGGTGGGGGTGGTGCAGGTGCCCTG CATGGGTTCCACTGCCCTAAAGAGGGTACAAAGGCCACACCTCCGTGTGTCTGCCAGGGCTGGGTTCAGA GCCTCCCTGGCTTCCTGGGCACTCTGCCTATCACACAGCGATTCCAATATGATCACATGTTCAGAACAGC CACACTTGTCAAAGCAAGGAGAGAACACTTCAGCAACGTTCAAAAACATGTCTCCAGGGATTAAAAAAAA AACACGGAAAGCTTCATTTTCCTCCCTAGTGGGGAAGTCTTCCTTACTCACAGCCTCTTCCTGAGTTGGT GTCTGTCGTGAAGCATTCTTTGGAAGCATAGTAAGCGGAGGGTTTAATTTACCATAGTCGTACACATTTG CACAGAATCTAAAGTTTGCAGTGCGTTTCATCATCTTTATGTGAATCTCATGCAGCTCTTACAGGGAAAA CAGGAAGAACTGGCCCCATTTTCTAAATGTGGAGACAGGAGGAGGTGAGATGGCTCGCCTCTGCCCAGCC GACTGGGAAATGCAGAGCTCAGAGAGACAGCAAGTCAGGAGATGTTGATCCAGGGAGCTTTTCACTCTGC CACCCACCCCATCGATGGAAGCACCGACATTATCAAGGCTGCATTTAGATTTCAAAACAAAAGCAAGCAA ACATGCCGGGCTCGTGGTATGCTGTTGTTTTAACCGAAATGATACAGCTCAAAGGGTGAGCAGCCATCAG TGCTGTGAGCGAAGCGTGATCACACATCTTGTATGTTTAGCAACTCAGGAGGTAGCTGAGCCTGGAAGTG ACTTTCCTGGTATGTAGCAATTCAGAGAATACAAAACCACATTCATTATCTGAAATGGGCTCAGCTGCTT CTGTGTTTTATCATATAGAGCTGGGAACTTTGATGTGTGTGGTGTGTGCGTGTTTTGCTGCATATACAGA TAATCACACAACTGGAGGCTTCAGCTTGGCTGTTTACACACACACACACACACACACACACCCCAGCAGA TACATCACACACAGACTTCTCACCTGTTACGTTACTAACAGTGGTGCTGGTTTGTTTGAACAGTGTTTGA ACTTATCAATAACCTTCCTCCAGAAGGCTCAGCATAGCTATAGCATATGCTGTGTGTGGAAAATATAACC TAAGAAACAGAATGGAATTGAATCATGACCACTATTGCTATGAGACAGAATCCACAGCGATGGAGTGGGT GCTGGGGTCAGACTGCCTGAGTGTGTACCCTAGGTGAGTCACATAATAGCTGGGTGAATGGCCAAGTTTT CACCTTTTTTCCCTCATTTGGAAAATGGGATGATAATAGGAGCTATCCTGAAGGGTGATGATGAGGGTTA AATTAACAATATAGGTAAAGGCTTAGAACAGTGTGTGGCAGATGGTAGGTAAGCCCTTAATAAATATAAA ATATCATTATTTGTGTCCATCATTTGAGATATATCTCACATCTTATCCAGAGGACTCCCCAAACACTCGT GTTGTCACTTCTCTTTCCTGTCTCTGGGTTCCTCTACAAGTCAGTTTTGTACCATGCAGTCTCGCATTTG ATGACATGCAGCATTACTTAGTTCTGTGAATGATTCGTGTGTCTTAATGTCACTTCCCCAACAGACTGTA AATTTCTTGATGGCAAGAACCATGTAACTGGTTTTTGTTAGATTTCTGCAACTCAAGACCCAATCCTGGG CAACTGTTGGACACTTAAACATCCTTCATCACTAGCTGCGTTCATCACTAGGAAAAACCAAAAACCAGGA ACAACCCGGATGTCCTTCAACAGGGGAGGGGTGACCCAGCCCGTGATGCGTCCGTCACACTGTGGATTGC TGCTCAGCAACGAGGAGGCACAGACTCGGTGCGGGAGCCAGCCTGGGTGACGCTCCGGAGAGCTACCCTG AGTGGAGAGGGGCAGTCCCACCGTGTGATTCCATTATTATCGCATTCTTGAAATGACAGAGTTGTGGAGG GGAGAACAGAGGAGTGGCTGCCGGAGTTTGGGGAGGGAATGGGGAAAGGGGGAAGAGCGGCATGCGGGAT CCTTGTGACGGAAGCGTTTTGTGTCGTGTGGGTGTCTGTCGGTTTCCTGGCTGTGATGCTGTGCCATTGT CTTGTGGGATGCTGCCATCGGTGGAAACTGGGTGGAGCATGTAGGGGACCTCTCTGTATGATTTCTTACA ACTGCATGTGAATTTACAGTGGTCTCAAAATAAACGATTTAATTAAACAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832802 unedited TCAGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGTAAAAAG AGATTGCTTGAGGCCACATGTTCTAGACTAGTCTGGGCAACATAGTGAGGCCCCATCTCT AGAAAAAGTTTTAAGTTAGTTGGGAGCAGTGGTGTGTGCCTATAGTCCTAGCTACTTGGG AGGCTGAGGTGGAAAGATAACTTTAGCCAAGGAGTTCTAGCCTGCAGTGAACTATGATAG TACCACTGCACTCCAGCCTGGGCAACAGAGCAAGGCTGTCTCTTAAAAAAAAAAAGGAAG CCACAGAGAGAGGCCAGGTAAGGACCCAGGGTCTCGTGGATTTTGCAATGGAGGTAAACA CAGTGTGGGCTGATGCAATCAGTGTTTGCTGCCCTTGGGCGCTTCGCTTCACCTCTAAGG GGCTCTGCAACGTGATAGAGTTGGGCCAGTGAATTTGTCAGGTGTCTTCTGGCTGTTACT TTTGTCTGTGTGTGAACCTTTGTTTTAGGCCATTTCTGTAGTTAGTGAACAGAGCAGATA GAGTCCAGTCATTTTGCAGAAATGTTTCCGTTTGGATGATGAAACAAGCTTGATTGCTGA AGCTGGTGCACTCATCCCATTGGCATGGTGCTAGCCCAGACCTTTGAAATACCCTGAGAC ACCTCTTGCCTGTTTATAGAAGTTGCTTCTGTCTGCCTGATAAGGGCTTGTTATTAACCA NGGAGGTGCATTTTAGCGACACTGGGCCATTTTTCTCTGCATACCAAGTCCTCCACATCA AAGAAGAAATTTAGGATGAAAGGTTCTTTTAAAACTTAGGCCACGTGCTGCAGCTATTGC CACTGCAAGGACCTGCGGCAGTTCTGTTGGAAATGGTTGTTCTCGATGTGTTCCATGCCA CATGTTGGGATAAGAATCTAAGTTTTGGAACTAACTACTGCATGAGACACTTTTTTTGTT GGGAATGCCCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL832802 unedited AACTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTAATTAAAT GCTTTATTTTGAGACCACTGTAAATTCACATGCAGTTGTAAGAAATCATACAGAGAGGTC CCCTATATGCTTTACCCAGTTTCCACCGATGGCAGCATCTTACAAGACAATGGTACAGTA TCACAGCTAGGAAACTGACAGACACCCACACGACACAAAACGCTTCCGTCACAAGGATCC CGCATGCTGCTCTTCCCCCTTTCCCCATTCCCTCCTTAAACTCTGGCAGCCACTCCTCTG TTCTCCTTTCTATAATTCTGTCATTTCAAGAATGTGATAATAATGGAATCACACGGTGGG ACTGCCCTTCTCCACTCAGGGTAGTTCTCTGGAGCGTCACCCAGGCTGGCTCCCGTATCG AGTCTGTGCCTCCTCGTTGCTGAGCAGCAATCCACAGTGTGACGGATGCATCACGGGCTG GGTCACCCTTCCCTTGTTGAAGGACATCCGGGTTGTTCCTGGTTTTTGGCTATTATCAAT AAAGCTGCTATAAACATTTTGTACGCACATGTGTGTCAATATACATCTTCACATCTTAAG TTTCAGCTGTTGGATCTAATACCCACTTACAATCCCATACCATCAGATTTCTCTTACTTT TTCCTAGTGATGAACGCAGCTAGTGATGAAGGATGTTTAAGTGTCCAACAGTTGCCCAGG ATTGGGTCTTGAGTTGCAGAAATCTAACAAAACCAGTTACATGGTTCTTGCCATCAAGAA AATTACAGGCTGTTGGGGAAGTGACATTAGACACCGAATCATTCCCGAACTAAGTAATGC TGCATGTCATCACAAGCGAGACTGCATGGTACAAAACTGACTTTGTGAGGAACCCCNAAA CCGGAAAGAGAATGACACNCCCATGGTTTGGGAGTTT
Restriction Sites	NotI-NotI
ACCN	AL832802
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL832802.1
RefSeq Size	3630 bp
RefSeq ORF	3630 bp
Locus ID	9747
Gene Summary	Positively regulates the plasma membrane cation channel TRPM8 activity. Involved in the recruitment of TRPM8 to the cell surface. Promotes prostate cancer cell migration inhibition in a TRPM8-dependent manner.[UniProtKB/Swiss-Prot Function]

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