CCDC132 (BC017888) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCDC132; VPS54L
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC017888, the custom clone sequence may differ by one or more nucleotides ATGCAAAAAATCAAATCTCTCATGACCCGACAGGGTCTGAAAAGCCCTCAAGAAAGCCTCAGTGATCTTG GTGCCATAGAGAGTCTCCGGGTCCCTGGAAAGGAAGAATTCAGGGAACTTCGAGAACAGCCAAGTGACCC TCAAGCTGAACAAGAGCTTATTAATAGTATTGAACAAGTATATTTTTCTGTGGATTCATTTGATATTGTT AAATATGAGCTGGAGAAGCTTCCACCTGTTCTCAATTTGCAAGAATTAGAGGCGTATAGAGACAAATTGA AACAACAGCAAGCTGCAGTATCTAAAAAAGTGGCAGATTTAATCCTTGAAAAACAGCCTGCTTATGTAAA GGAACTCGAAAGAGTTACCTCATTGCAGACAGGTCTTCAATTAGCTGCTGTTATCTGTACAAATGGGAGA AGACACTTGAATATTGCAAAGGAAGGTTTTACTCAAGCTAGTTTAGGCCTTCTTGCAAATCAAAGGAAAC GTCAGTTGCTGATTGGACTTCTGAAATCTCTGAGAACTATAAAAACATTGCAAAGAACAGATGTACGGTT AAGTGAAATGCTGGAGGAGGAAGATTATCCAGGAGCTATTCAGTTGTGCCTTGAATGTCAAAAAGCTGCC AGCACTTTTAAACATTACAGTTGTATAAGTGAACTGAATTCAAAGCTGCAAGATACTTTGGAACAGATTG AGGAACAGCTGGACGTAGCTCTTTCCAAAATCTGCAAGAATTTTGACATTAACCATTATACCAAGGTTCA ACAAGCTTATCGACTTCTTGGAAAAACACAGACAGCAATGGATCAACTTCATATGCACTTCACCCAAGCC ATTCACAACACCGTGTTTCAAGTTGTTCTTGGTTATGTGGAACTATGTGCAGGAAACACAGACACAAAAT TCCAAAAGCTGCAATATAAGGATCTCTGTACAGTATGTAGTGACTTAATTACTATTCATATATCTCTCCT TTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017888 unedited GGGTACACCAATTTGTNATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGGG CCAGCTGTGTAGTGGCTCGGTGTGATTTGTTAGCTCTTTGAGGCAGGGTACCCTCCTCAG GATTTCGATATGCAAAAAATCAAATCTCTCATGACCCGACAGGGTCTGAAAAGCCCTCAA GAAAGCCTCAGTGATCTTGGTGCCATAGAGAGTCTCCGGGTCCCTGGAAAGGAAGAATTC AGGGAACTTCGAGAACAGCCAAGTGACCCTCAAGCTGAACAAGAGCTTATTAATAGTATT GAACAAGTATATTTTTCTGTGGATTCATTTGATATTGTTAAATATGAGCTGGAGCTTCCA CCTGTTCTCAATTTGCAAGAATTAGAGGCGTATAGAGACAAATTGAAACAACAGCAAGCT GCAGTATCTAAAAAAGTGGCAGATTTAATCCTTGAAAAACAGCCTGCTTATGTAAAGGAA CTTGAAAGAGTTACCTCATTGCAGACAGGTCTTCAATTAGCTGCTGTTATCTGTACAAAT GGGAGAAGGTATTGCTTTTGATGATATTTTGTTCTTTAAGCTGGACAATATTTCAAGAAT AACTATGGAATCTATAATTTATAATTGATTTTTTAAATTATGGATGTGGTTTCTAGAATA TCATTCCTTAAGTGAACATATATCATGATCTAAAGACTACACTAAGAAGGTTTGTGGGAA ATATCTCTAAGTGTACTTATGGGTTTTAAAAAATTACTATTNTTATATTATTATNACTAA TGCTCCACAAACTGTTTGTCGTAAAGCACCTACTTTTTCCTTTCTTAAATTTCCAATCTG TTACAAGCTGTGATTTTTTAAAATGCACTAAAACAAATTCTTTGCAAATGAATTAAGAAG GAAGACATATACAGCCTAATTTTTTTAATATTAGAGCCCA
Restriction Sites	NotI-NotI
ACCN	BC017888
Insert Size	5000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017888.1, AAH17888.1
RefSeq Size	1216 bp
RefSeq ORF	984 bp
Locus ID	55610
Gene Summary	Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane. Within the EARP complex, required to tether the complex to recycling endosomes. Not involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).[UniProtKB/Swiss-Prot Function]

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