ANKRD57 (SOWAHC) (AF085927) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HCCS1; hVps53L; PCH2E; pp13624
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF085927, the custom clone sequence may differ by one or more nucleotides GAACGCCCAGGAAGTCCATACTCTCAAACCCCTTTCTGCCCACTCACCTTCAGGTCCACAGCACTTTCCA GCGTCATCTGAAATAGCAAAGGTGATGTGCAGCATCCCAGTGATGTGTTCCTGTGTTATGAGAAGGAGTC TGGGGTTCTGGATGCCTCAGGAACAGAATATGGACCTTAAAGACCAGGCTGAGAGCCTGGCCTGGCACGC AGCCTGCCCGCCCCCGCGCCCTTCCTGTCCACCGCGGGCTCTCTGCAGCAGGGCTTGCCGAAGAGACTGC TCCCGACCTCTCATGTCATCCTGCATCCAGAGAACGGCAGCATATCAAGCCGTTAACCCTGATTCACTCG GTTCCCTTGAAACCAGGCCTCAGTTAACCATGACCACAGCTACAGGCAAACCGCTGCTGTTGCTGCCTGC TTTTGCTGAAGAATGGTATCTTGCCAGGCACGGCGGCTCATGCCTGTAGTCGCAACACTTTGGGAGACCA AGGCAGGAGGATCACTTGAGTCCAGGAGTTTGAGACCAGCCTGGGCAACATACAGATGTCTACAAAAATA AAAATTTGCAAGGTGAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF085927 unedited GCAGGAATAGGTAATACGATTTACTATAGGGNNCGGCCGCGAAATTCGGCACGAGGAAAT ACAATTAAAAAAAAAAAAAAAAGCAGGCAGGTGGNNCGGGTGCCTGTAATCTCAGCCTCC TGAGTATCGGATTACAGGCGTGAGCCACCGAGCCCAGCCCCTTCCTCCATTTCTTTACTC CCACCCTGCTCCTGGTTTCTAGGGAAGGGAAACCAGGAAGTTCACTTGGAAGACGTTTCC TAGGTTTTCTCATCAGATGAAGCCATGGGAACGCCCAGGAAGTCCATACTCTCAAACCCC TTTCTGCCCACTCACCTTCAGGTCCACAGCACTTTCCAGCGTCATCTGAAATAGCAAAGG TGATGTGCAGCATCCCAGTGATGTGTTCCTGTGTTATGAGAAGGAGTCTGGGGTTCTGGA TGCCTCAGGAACAGAATATGGACCTTAAAGACCAGGCTGAGAGCCTGGCCTGGCACGCAG CCTGCCCGCCCCCCCCGCCCTTCCTGTCCACCGCGGGCTCTCTGCAGCAGGGCTTGCCGA AGAGACTGCTCCCGACCTCTCATGTCATCCTGCATCCAGAGAACGGCAGCATATCAAGCC GTTAACCCTGATTCACTCGGTTCCCTTGAAACCAGGCCTCAGTTAACCATGACCACAGCT ACAGGCAAACCGCTGCTGTTGCTGCCTGCTTTTGCTGAAGAATGATATCTTGCCANGCAC GGCGGGTCATGCCTGTAGTCGCAACACTTTGGGAGACCAAAGCACGANGGTCACCCTGAG TTCACGAGTTTGAGACCAACCTGGGCAACATACAGATGTCTACAAAATTAAAAATTGCAA GGGGTGGTGGCATGCACCTGTAGTCCCACTGCTAGGGAAGCTGAGGTGGGCANAATGCTT GAACTCAGGAGTTTGAGACACGCCTGGCCACCTGGCAAAACCACAATACAAAAATGGCTG GTGGGGTGGCTCAG
Restriction Sites	NotI-NotI
ACCN	AF085927
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF085927.1
RefSeq Size	585 bp
RefSeq ORF	585 bp
Locus ID	65124

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