NARG2 (ICE2) (BC020918) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BRCC1; NARG2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC020918, the custom clone sequence may differ by one or more nucleotides AGGGGCGGGAGGAGGGAACATGGCGGCCGGGCCAGGACCCTTTGCTCCTGGCTCTGCGCGGCTCCTACCC CTGCTTTGCTTGCCCTTTTACCTGCTTTTCTGGAACCTTGTTTCCCCTCTCTTTGCGTCCCCTTTCCCTC AGCCGTTTCTTTTGCCTTCCTCGACTATTCCCCCCCAGCTCCACACTGGGTCGAAGTGTGAGGGGTAGAT TAAGTGGGAGCCGGTCGGGCCCGTTCGGTGCCGAGAGACCGACTCCTAAATTTGATTAGGTTCTCGCATG TCACTTTATCAGTCTGCCTGGGGTCACAGGAGAAGTACTGAACATGAGAGATCAAGCCTGATTTGTAGTC CTGATGATGTCTCTGAGTAAGTTCTGGAGGGATCCTGCCTCTTGGAGCCTTCGCAGCCAGGCAGCTGTGA ACTGTGAGCTAGAGTGAAGCAGAAATCTAGGAAGATGAGCTCCAAGATGGTCATAAGTGAACCAGGACTG AATTGGGATATTTCCCCCAAAAATGGCCTTAAGACATTTTTCTCTCGAGAAAATTATAAAGATCATTCCA TGGCTCCAAGTTTAAAAGAACTACGTGTTTTATCCAACAGACGTATAGGAGAAAATTTGAATGCCTCAGC AAGTTCTGTAGAAAATGAGCCGGCAGTTAGTTCAGCAACTCAAGCAAAGGAAAAAGTTAAAACCACAATT GGAATGGTTCTTCTTCCAAAACCAAGAGTTCCTTATCCTCGTTTCTCTCGTTTCTCACAGAGAGAGCAGA GGAGTTATGTGGACTTGTTGGTTAAATACGCAAAGATTCCTGCAAATTCCAAAGCTGTTGGAATAAATAA AAATGACTACTTGCAGTACTTGGATATGAAAAAACATGTGAACGAAGAAGTTACTGAGTTCCTAAAGTTT TTGCAGAATTCTGCAAAGAAATGTGCGCAGGATTATAATATGCTTTCTGATGATGCCCGTCTCTTCACAG AGAAAATTTTAAGAGCTTGCATTGAACAAGTGAAAAAGTATTCAGAATTCTATACTCTCCACGAGGTCAC CAGCTTAATGGGATTCTTCCCATTCAGAGTAGAGATGGGATTAAAGTTAGAAAAAACTCTTCTCGCATTG GGCAGTGTAAAATATGTGAAAACAGTATTTCCCTCAATGCCTATAAAGTTGCAGCTGTCAAAGGACGATA TAGCTACCATTGAAACGTCAGAACAAACAGCTGAAGCTATGCATTATGATATTAGTAAAGATCCAAATGC AGAGAAGCTTGTTTCCAGATATCACCCTCAGATAGCTCTAACTAGTCAGTCATTATTTACCTTATTAAAT AATCATGGACCAACGTACAAGGAACAGTGGGAAATTCCAGTGTGTATTCAAGTAATACCTGTTGCAGGTT CAAAACCAGTTAAAGTAATATATATTAATTCACCACTTCCCCAAAAAAAAAAAAAAAAAAAAAAGAAAAA AA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020918 unedited TCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGCGCGGCC ACATCCTTTAAATATGGTCTTTCTTGGGCGCGCGCGACAATGTGAGGAGTGGGGTGGAGC GTGTGTGGTGTGTGGCTGCGGCCTGGGCAAGAGCCGCCGCGGACCATGAGCTGAGTAAGT TCTGGAGGGATCCTGCCTCTTGGAGCCTTCGCAGCCAGGCAGCTGTGAACTGTGAGCTAG AGTGAAGCAGAAATCTAGGAAGATGAGCTCCAAGATGGTCATAAGTGAACCAGGACTGAA TTGGGATATTTCCCCCAAAAATGGCCTTAAGACATTTTTCTCTCGAGAAAATTATAAAGA TCATTCCATGGCTCCAAGTTTAAAAGAACTACGTGTTTTATCCAACAGACGTATAGGAGA AAATTTGAATGCCTCAGCAAGTTCTGTAGAAAATGAGCCGGCAGTTAGTTCAGCAACTCA AGCAAAGGAAAAAGTTAAAACCACAATTGGAATGGTTCTTCTTCCAAAACCAAGAGTTCC TTATCCTCGTTTCTCTCGTTTCTCACAGAGAGAGCAGAGGAGTTATGTGGACTTGTTGGT TAAATACGCAAAGATTCCTGCAAATTCCAAAGCTGTTGGAATAAATAAAAATGACTACTT GCAGTACTTGGATATGAAAAAACATGTGAACGAAGAAGTTACTGAGTTCCTAAAGTTTTT GCAGAATTCTGCAAAGAAATGTGCGCAGGATTATTATATGCTNTCTGATGATGCCCGTCT CTTCACAGAGAAAAATTTAGAGCTTGCATTGAACAAGTGAAAAAGTATTCAGNATNCTAT ACTCTTCACGAGGTCACCAGCTTATGGNATTCTTCCATTCGAGTTGAATGGGATTAAGTA GAAAAACTCTCTCCATGGGCATGTAAAAATGTGAACAATTTTTCTCAAGCCATAAGTGCA GTGTCAAGACT
Restriction Sites	NotI-NotI
ACCN	BC020918
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020918.1, AAH20918.1
RefSeq Size	1472 bp
RefSeq ORF	1472 bp
Locus ID	79664
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein component of the little elongation complex (LEC), which plays a role in small nuclear RNA (snRNA) transcription. The LEC regulates snRNA transcription by enhancing both RNA Polymerase II occupancy and transcriptional elongation. The encoded protein and other LEC components have been shown to localize to Cajal bodies, which are sites of ribonucleoprotein (RNP) complex assembly. Pseudogenes of this gene have been identified on chromosomes 3 and 4. [provided by RefSeq, May 2017]

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