RPL28 (BC011582) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	60S ribosomal protein L28; FLJ43307; ribosomal protein L28
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC011582, the custom clone sequence may differ by one or more nucleotides ATGTCTGCGCATCTGCAATGGATGGTCGTGCGGAACTGCTCCAGTTTCCTGATCAAGAGGAATAAGCAGA CCTACAGCACTGAGCCCAATAACTTGAAGGCCCGCAATTCCTTCCGCTACAACGGACTGATTCACCGCAA GACTGTGGGCGTGGAGCCGGCAGCCGACGGCAAAGGTGTCGTGGTGGTCATTAAGCGGAGATCCGGCCAG CGGAAGCCTGCCACCTCCTATGTGCGGACCACCATCAACAAGAATGCTCGCGCCACGCTCAGCAGCATCA GACACATGATCCGCAAGAACAAGTACCGCCCCGACCTGCGCATGGCAGCCATCCGCAGGGCCAGCGCCAT CCTGCGCAGCCAGAAGCCTGTGATGGTGAAGAGGAAGCGGACCCGCCCCACCAAGAGCTCCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC011582 unedited GCCGCGAATTCGGCACCAGCCGCTGCGGAGGGAGCCGCCGCCATGTCTGCGCATCTGCAA TGGATGGTCGTGCGGAACTGCTCCAGTTTCCTGATCAAGAGGAATAAGCAGACCTACAGC ACTGAGCCCAATAACTTGAAGGCCCGCAATTCCTTCCGCTACAACGGACTGATTCACCGC AAGACTGTGGGCGTGGAGCCGGCAGCCGACGGCAAAGGTGTCGTGGTGGTCATTAAGCGG AGATCCGGCCAGCGGAAGCCTGCCACCTCCTATGTGCGGACCACCATCAACAAGAATGCT CGCGCCACGCTCAGCAGCATCAGAACACATGATCCGCAAGAACAAGTACCGCCCCGACCT GCGCATGGCAGCCATCCGCAGGGCCAGCGCCATCCTGCGCAGCCAGAAGCCTGTGATGGT GAGAGGAAACCCGACCCCCCCCACCAAGAGCTCCTGAGCCCCCCTGCCCCCGAGCAATAA AGTCAGCCTGGCTTTCTCNNNNANAAANNNNNNNNNANAAAAAAAAAAAAANAACCTCGA CTCTAGATTGCGCCCGGGTCATAGCTGTTCCTGAAAAATCCCGGGGGGCATTCTTGGAAC CCCTCCCAAGGCCTTTCCTGGCCCTGAAGTTGCCCTCCAGGGCCACCACCCCTTGTCTAA AAAATTAAGTGCATATTTGCTGAAAGGGGTCTCTAATAGGGGGGGGGGGGGGGGGNNA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC011582 unedited NNNCGGTAAAAGCTAGNAACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTGAAAAAACCAGCTGACTTTATTGCTCTGGGGGCAGG GGGCTCAAGAACTCTTGGTGGGGCGGGTCCCCTTCCTCTTCACCATCACAAGCTTTTGGC TGCCCAAGATGGCGCTGGCCCTGCGGATGGCTGCCATGCCCAAGTCGGGGCGGTACTTGT TCTTGCGGATCATGTGTCTGAAGCTGCTGAACGTGGCCCCAGCATTCTTGTTGATGGGGG TCCCCACATAAGAAGTGGCAGGCTTCCCCTGGCCGGATCTCCGCTTAATGACCACCACGA CACCTTTGCCGTCGGCTGCCGGCTCCACGCCCACAGTCTTGCGGTGAATCAATCCCTTGT AACCGAAAGAATTGCGGGCCTTCAAGTTATTGGGCTCAATGCTGTAAGTCTGCTTATTCC TCTTGATCAAGAAACTGGAGCAATTCCCCACGACCATCCATTGCAAATGCCCAAACATGG GGGCGGCTCCCTCCACAACGGCTGGTGCCCAATTCCCGGCCGCCCTATAGTGAGTCCTAT TACAAAATTCTGACGGTTCACTAAACGAACTCTGCTTATATAAACCTCCCACCGTACACG CCTACCGCCCATTTGCGTCAACGGGGGCGGGGTTATTACCACATTTTGGAAAGTCCCGTT GATTTTGGTGCCCAAACAAACTCCCATTGACGTCAATGGGGGTGGAGACTCTGGAAATCC CCGTGAGTCAAACCGCTATCCACGCCCATTTGTTGTACTGCCAAAACCGCATCACCATGG TAATAAGCGATGACTAATACCGTAAATGTACTGCCCAGTATAGAAAGTCCCGGTTAGGTC ATGTTACTGGGCATAATGGCCAGGCGGGGCCATTTACCGTCATTGGACGTCA
Restriction Sites	NotI-NotI
ACCN	BC011582
Insert Size	600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011582.1, AAH11582.1
RefSeq Size	4222 bp
RefSeq ORF	414 bp
Locus ID	6158
Domains	Ribosomal_L28e
Protein Pathways	Ribosome
Gene Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]

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