SART3 (BC015627) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DSAP1; P100; p110; p110(nrb); RP11-13G14; TIP110
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC015627, the custom clone sequence may differ by one or more nucleotides CTTCTGTCCGAAGTTGTGTCGGAACGCATGCCATTATAATGAGTTTTTATATCTGGTGGGAGCTTTTACA AGGGTATAAAATAATGTGAATTTAGTGTATGTGGAGATTTTCCTGTTATCTAAGTTCCCAGTTTCTCTTT CAATGGCAAGAAAACGGGTTTTTGATAAGGCGCGCGCCGCGGGCAGCTTCTGCCTCAGCGGGTCTTCGGC GGCCGCGGGAAGACCCGAGAGTCATTAGAAGCGCAAGATGGCGACTGCGGCCGAAACCTCGGCTTCAGAA CCCGAGGCTGAGTCCAAGGCTGGGCCCAAGGCTGACGGAGAGGAGGATGAGGTTAAGGCGGCTAGGACAA GGAGAAAGGTGTTATCGCGGGCTGTGGCCGCTGCGACATACAAGACCATGGGGCCAGCGTGGGATCAGCA GGAGGAAGGCGTGAGCGAGAGCGATGGGGATGAGTACGCCATGGCTTCCTCCGCGGAGAGCTCCCCCGGG GAGTACGAGTGGGAATATGACGAAGAGGAGGAGAAAAACCAGCTGGAGATTGAGAGACTGGAGGAGCAGG TGGGCCCGGGGGTGGGCAGCGGGCATCTTCCAGTCTTTCAGGTCCTCGGGTCCCCATGTCCTGGCCCGCC TCCCTAGTCCTCGGCGGCGATAAAGCCGCCAAGGCTGCAAACCCGTTTTTCGTGACAGTAGCTCCTGTCG GATTTGAGTGCTTACTGCCTGCCCATTGCCTTGCAAACGTTATCTCATTGAATCCTCGCCACGACCCTGT GAAGGAAGCCCTGTTATCTATCCCCATTTTACTGGAGGAAACTGAGGCTCGTGGAGGAAAGTCTCTTGCT CAAGATAACACTTCCTGCAAGTCAGGCCTTGTACTTTGATCCCATTCCAGAACTCCCACTCTTAACCACA CGTCCAACAGTAGAAATAAACTCATTCGCAAGAGAGTGTTATTTTGTTATGTAGGAATGACGGCGATGAT GAAACTTGTGGTAAGAAAAATATTGACCGCGATGGTGATAATAGAAATGGAAAGGTTTCTGGTTCTGGAG GTTAAGGCCTCAGTTCTAGCTGTTCAACTCTTGTATGTGCTTGTAAGAATTTCTTAATTTCTCTCACCCT CTCGACTTCCTTATCTGCAGATTGGGAGCAACAATTTCTGCATAATATAATTGTTGTAACAGTAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC015627 unedited GTTCACATATAGNATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGCGCAAGA TGGCGACTGCGGCCGAAACCTCGGCTTCAGAACCCGAGGCTGAGTCCAAGGCTGGGCCCA AGGCTGACGGAGAGGAGGATGAGGTTAAGGCGGCTAGGACAAGGAGAAAGGTGTTATCGC GGGCTGTGGCCGCTGCGACATACAAGACCATGGGGCCAGCGTGGGATCAGCAGGAGGAAG GCGTGAGCGAGAGCGATGGGGATGAGTACGCCATGGCTTCCTCCGCGGAGAGCTCCCCCG GGGAGTACGAGTGGGAATATGACGAAGAGGAGGAGAAAAACCAGCTGGAGATTGAGAGAC TGGAGGAGCAGTTGTCTATCAACGTCTATGACTACAACTGCCATGTGGACTTGATCAGAC TGCTCAGGCTGGAAGGGGAGCTTACCAAGGTGAGGATGGCCCGCCAGAAGATGAGTGAAA TCTTTCCCTTGACTGAAGAGCTCTGGCTGGAGTGGCTGCATGACGAGATCAGCATGGCCC AGGATGGCCTGGACAGAGAGCACGTGTATGACCTCTTTGAGAAAGCCGTGAAGGATTACA TTTGTCCTAACATTTGGCTAGAGTATGGCCAGTACTCAGTTGGTGGGATTGGTCAGAAAG GTGGCCTTGAGAAAGTTCGCTCCGTGTTTGAAAGGGCTCTCTCGTCTGTTGGTTTACATA TGACCAAAAGACTCGCCCTCNTGGAGGCTTACCGAGAGTTTGAAAGTGCGATTGTGGAAG CTGCTNCGCTTGAGAAAGNCACAGTCTTTTCCCGCGACAGTTGGCGATCCCACTCTATGA TATGGAGGCCACATTTGCACAGTTGAAGAATGGTCAGAAGACCCATACCANNAGTCAGTA TTCAGAACTATACAAAGCCTACAGCAGCTGGAGAATATAACCCTATGAAGAACACTNTG
Restriction Sites	NotI-NotI
ACCN	BC015627
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC015627.1
RefSeq Size	1217 bp
RefSeq ORF	1217 bp
Locus ID	9733
Gene Summary	The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq, Jul 2008]

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