RCN1 (AF318337) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF318337, the custom clone sequence may differ by one or more nucleotides ATGGAGGTTTCATATACACCACTTACAGTTTTCAGCTCCTCAGTAGTGACAAAGCCATACCCATCATTGT CGATTCGATCAACAATCTTCCCTAGCCTCTCCTTGCTCTCGTCCGGGGTGAGCTCGTCGAAGTTCTTGGA GTCCTCCTTGCCCAGGAAGGCCTCGTGGTCGTACTGGAAGCTCTGGTTGTCCTCAGGGGGCCGCTCGCCC AGCTCCGAGTCGGGCCGCACCACGCGCTCTTTGCGCACCGTGGGCTTGGCCCGCAGAACCCGCGGCGCCA GCACCAGCGCCAGCAGCAGCCCCAGGGCTAACCCCAGGCGGCCACCGCGCGCCATCGTCCCGAGGAGAGG GCGGCCGGGAGGGAGACGCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF318337 unedited CTCATTAGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGTCTTGGACCGCTG TCGGATAGTAAATCCCAAGTAAGGTACCTGCCGTCGGCAGATTTGAGCTTTCTTCTTGGA CACCTAATACCCAGAGTCCTCCAGGCTCCGGTAGATTGCAAATGACCTGCTTTCTTTCTG TTCCCGGGCGGCATCGGACCCGTCGGAGAGTAAATCCCAAGTAAGGTACCTGCCGTTGGC AGATTTGAGCTTTCTTCTTGGACACCTAATACCCACAGTCCTCCAGGCTCCGGTAGATTG CAAATGACCTGCTTTCTTTCTGTTCCCGGGTGGCATCGACCCGTCGGAGAGTAAATCCCA AGTAAGGTACCTGCCGTTGGCAGATTTGAGCTTTCTTCTTGGACACCTAATACCCACAGT CCTCCAGTAAATCCCAAGCAAGGTACCTGCCGTCAGCAGATTTGAGCTTTCTTCTTGGAC ACCTAATACCCACAGTCCTCCAGGCTCCGGTAGATTGCAAATGACCTGCTTTCTTTCTGT TCCCGGGCTGCGTTCGGACCCCTGTGGGATAGTAACTCCCAAGTAAGGTACCTGCCGTCG GCAGATTGGAGCTTTCTTCTTGGAGACCTAATACCCACAGTCCTCCAGAAAAACAAACAA AGACATGGATTTACTGTGCATATTAGCAGATCCATACTGGAAAATGCATGGAGGTTTCAT ATACACCACTTACAGTTTTTAGCTCCTCAGTAGTGACAAAGCCATACCCCATCATGCCGA TTCGATCAACAAACTTCCCTAGCCTCTCCTTGCTCTCGTCCGGGGTGAGCTCGTCCAAAT TCTTGGAGCCTTTCTGCCCAGGAAGCCTCATGTCCTACTGAAGCTCTGGTGTTCTCAGGG GCCGTCGCCAGCTCGATTGGCCGCACANCGCTCTTGCGCCCGGGGCTTGCCCGCAAACCG C
Restriction Sites	NotI-NotI
ACCN	AF318337
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF318337.1, AAL55844.1
RefSeq Size	1581 bp
RefSeq ORF	1581 bp
Locus ID	5954
Gene Summary	Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.[provided by RefSeq, Jan 2009]

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