TMEM85 (EMC4) (BC002583) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ90746; MGC24415; PIG17; proliferation-inducing gene 17; transmembrane protein 85
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC002583, the custom clone sequence may differ by one or more nucleotides ATGACGGCCCAGGGGGGCCTGGTGGCTAACCGAGGCCGGCGCTTCAAGTGGGCCATTGAGCTAAGCGGGC CTGGAGGAGGCAGCAGGGGTCGAAGTGACCGGGGCAGTGGCCAGGGAGACTCGCTCTACCCAGTCGGTTA CTTGGACAAGCAAGTGCCTGATACCAGCGTGCAAGAGACAGACCGGATCCTGGTGGAGAAGCGCTGCTGG GACATCGCCTTGGGTCCCCTCAAACAGATTCCCATGAATCTCTTCATCATGTACATGGCAGGCAATACTA TCTCCATCTTCCCTACTATGATGGTGTGTATGATGGCCTGGCGACCCATTCAGGCACTTATGGCCATTTC AGCCAAGAATGGAGTTCAGTGGTGGAGGACTGCTTTTGTGAACATGAGAAAGCAGCGCCTGGTCCCTATG TATTTGGGTCTTATTTACATCCTTCTTTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC002583 unedited TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAACGCTGGTGGGCCT GTTGTGGAGTACGCTTTGGACTGAGAAGCATCGAGGCTATAGGACGCAGCTGTTGCCATG ACGGCCCAGGGGGGCCTGGTGGCTAACCGAGGCCGGCGCTTCAAGTGGGCCATTGAGCTA AGCGGGCCTGGAGGAGGCAGCAGGGGTCGAAGTGACCGGGGCAGTGGCCAGGGAGACTCG CTCTACCCAGTCGGTTACTTGGACAAGCAAGTGCCTGATACCAGCGTGCAAGAGACAGAC CGGATCCTGGTGGAGAAGCGCTGCTGGGACATCGCCTTGGGTCCCCTCAAACAGATTCCC ATGAATCTCTTCATCATGTACATGGCAGGCAATACTATCTCCATCTTCCCTACTATGATG GTGTGTATGATGGCCTGGCGACCCATTCAGGCACTTATGGCCATTTCAGCCACTTTCAAG ATGTTAGAAAGTTCAAGCCAGAAGTTTCTTCAGGGTTTGGTCTATCTCATTGGGAACCTG ATGGGTTTGGCATTGGCTGTTTACAAGTGCCAGTCCATGGGACTGTTACCTACACATGCA TCGGATTGGTTAGCCTTCATTGAGCCCCCTGAGAGAATGGAGTTCAGTGGTGGAGGACTG CTTTTGTGAACATGAGAAAGCAGCGCCTGGTCCCTATGTATTTGGGTCTTATTTACATCC TTCTTTAAGCCCAGTGGCTCCTCAGCATACTCTTTAACTAATCACTTCTGTTAAAAAGAC CAANAGACTTTTCTCCATGGTGGGGTGACAGGTCCTANAANGGACATGTGCATATTACGA CAAACACAAGAAACTTACCCATACCCAGGCTGAAAATATGTANA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC002583 unedited NCCGGGTATACCATGTACCGCGCCGCTTCTAGGATCGATTTTTTTTTTTTTTTTTTGCTT GTTCTCAACCATTTAATTATGAAAGGAATGCTGCAACAGTTCTTGATTTAGCAGCAGTTA TTCTCTTGTTTACATAGTTATGTTTTTTTGTTGTTGTTTTGCTCTGTACTGGAAACAAAA ATAAAGTTTTCTACATTATTTTCAGCCTTGGGTTATGGTATAGTTTCTTTGTGTTTGTCG TAATATGCACATTGTCCTTCTAGGACCTGTCACCCCACCATGGAGAAAAGTCTTTTGGTT CTTTTTAACAGAAGTGATTAGTTTAAGAGTATGCTGAGGAGCCACTGGGCTTAAAGAAGG ATGTAAATAAGACCCAAATACATAGGGACCACGCGCTGCTTTCTCATGTTCACAAAAGCA GTCCTCCACCACTGAACTCCATTCTCTCACGGGGCTCAATGAAAGCTAACCAATCCGATG CATGTGTAGGTAACACTCCCATGGACTGGCACTTGTTAACAATCAATGCCCAACCCATTC CTGTTCCCAATGAGATAAACCCAACCCTGAACACAACTTCTGGCTTGAACTTCCTACTTC TTGGCAGGGGCGTGAACTGCCATAAAAGCCTGAACTGGTGGTCCGGCCATTCTACCACCA TTTTAGCCACGGGGAAGATGCATAAATTTTCGCCTGCCCTCCCCCCACACGAACGCATAC CCCGCCACCTGCCTGCGGGCCGCGCACGGCGTGGTCCCCTGTCCTCCTTTTCCCCCCGTA TCCCGGCCTTTTCTTTGCCCACCTTTTTCATGACCCCTCCGCCTTCATTATCCCACACTC GTCTAAACATAGACATCCCTGGCGACTCGTCACGGGTCCTGTCCCCG
Restriction Sites	NotI-NotI
ACCN	BC002583
Insert Size	5000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002583.2, AAH02583.1
RefSeq Size	853 bp
RefSeq ORF	450 bp
Locus ID	51234
Protein Families	Transmembrane
Gene Summary	May mediate anti-apoptotic activity.[UniProtKB/Swiss-Prot Function]

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