FBXL17 (BC018548) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Fbl17; Fbx13; FBXO13
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC018548, the custom clone sequence may differ by one or more nucleotides AGGACAAAACCTGAAAGAGCTATATTTGGTGTCCTGTAAAATCACAGATTATGCACTGATAGCCATTGGG CGATACAGCATGACAATAGAGACTGTGGATGTCGGATGGTGTAAAGAAATCACAGACCAAGGAGCCACCC TGATTGCACAGAGCAGCAAGTCTCTGAGATATTTGGGGCTGATGAGATGTGATAAAGTCAACGAAGTGAC GGTGGAACAGCTGGTGCAGCAGTACCCCCACATCACCTTCAGCACCGTCCTGCAGGACTGCAAGAGGACC TTGGAGAGAGCCTATCAGATGGGCTGGACCCCCAACATGTCTGCCGCCTCCTCCTAGCGCTCCTGCCTCG CCTAGTCCACTGGGATCATTCAGCAGAGCAGAGGAGAATTGTACATTTGGGGAGCTGATCTCTCGGAAGG GTTTTAACTGTCACCTGTCTGTGTGTGTTCAAGTATGTGTATTTGTGTCTTGTTTGCATACTGCATACCA AAAGTGCAGTTGTCATTTGTTCCCAAGTGAGCTGGCTTTTTTTCTTAGAAATTAAAGATTATGAAAGCCA CAAACTTTTTAGTTTTTAACTTCAAAGGCTTCTTTCTCTTTAAAAAAAAATTATTTTTAATATAGAGTCA AAAAATTGGATGTATTATTTTGAGCTTCTAATTGCTGCCACTTGGAGATGTCCAAGTAAGAAGGCCTTCT TTTTACATGGGATGAATTGTGCACTTCTACTGATGATGACTACAGCAGAAGTGATGTTATACGTACAGGC ACTTACACTCTCTCACATATTCACACGTGTGCACATCTACACCAGGGCTCTGATCTACTCTAGATCGTTT CCTGACAGAGAAGCCTTCTTCCAGGCCACTAAGGTAGCATGTTATCCACTGTGGCACTGCCTAACTCCGT GTATCCTCGGGTGGCCAACTTGCTATCTGGTGGGTGACTTGGGGACTAGAAAGAATAAAGCCTTTTCTAA ATTTGTTATATAGAGGCAACAGTCTGAACTCTCCTGGCCAGCTCACGAAAGTTAATTATACATTATTCTA CAAATGCATTCTGTTGAGCACTAAATATTTTAGTTGGTTTTATGTTAACATTGTGTTAGCTTTTATTTCC TGGATAGTAACTGGTCAGGATATATTTTAATGATATTTGAAACTAATCTTAGCATTGAAGCCACCAATGT AATTAATGGTGTGCAGTTATTTTAAAAACTACAGATCATTTCATTATTTTCTTAATTTTGGTAATTATTA AAGTCTATTTCTGAGATACGAGAGGGTAGTCACATTAATAGAAGATTCATGAGTGAGATATAAGTAGACA ATCTTTCTACTGTTGTTGCTCTTTTTGCTTTGCTTTGGGTTCTTGAACAGGTTTTTTGGATATATGCATG TTGTCATTTTGGACTAGTTCTCATGCTACTGCAAAGATCTATTGCTAGCTAATATATGAGTTAAGAAAGA AAATTAGAAAATCTGTTCATTGAAATTCTGTTTACACTTGTCAGAAATAAGCATTTCTTTGTTTAATTAA GGATTGCCAGCTTCAGTTAAGACAAGACCTATCAGTAAATGAATATATGTTTTGATTTAACACATCTTTA CAATTAATTCATAATAGGGACTCAGGCTCAAATAACTGTTCCTGTGTCTAGATTTATAATGAGGACATCA GGGAGCCTTTTTAAGCTAAAGAGTGATTATCTTTCACAGTAGAACTAGAAACAAAACCCTAACTTGTGAA GTTGCTATTTATTTTTCCCTGAGTGAATATAACTGTTATTTGGTCTTTAACAGATATACTGAGGTTTGAG TTTCACTTTCTTTAAGTCTCACATTGCTAACATGACATTAGCTGTAAGAAACAAGATAGCTTTACATTTG AATGCCATTTACTGATTTCTAATGATTCTCTTTTTTTGTAAACAACTGTTTCCTTTGATTATGTCAACAT GTTTCTGATGTGGCATTTTTGTTTTAATCCCAATCGGTTAGGAAAATCTAGTCAGTAAGCGCCATGAGTA TTCTTCCCATAACTAAATGGGTTTCTAATTTAATTTGAAGGGAATAAAATTTGTGTAAGAAAAAAGCTGT TTAACATTAATAAACTATATTTCTTCTTACACATTGAACTGTATCATTTTCATATCCACTTCCCCACCAC TGAAATATCTTATTTCACAATATTTTAACTGATTTTTAATCCAAAACTAATTTATAAGACAGTATGTATA GTAATAGTAGCTTGAGTGAATATGCAAAAGTTTAATTTTTATATATGTCATACTATATTTTAAATAGTTA AAAAAGACAAAAGAAGGGAGAGCCATCTATGATATAGATGGTACCATTTCAGTTCAAAGTTGTAATAACC TTTGGAGTGTTACAGTTTGGTTGTCAAATGGTTTCAGAATGTATAATTGATTTTTTTAAATGTTGCATTG TTTGAATCTAAAGTACAGCACTATAACATGCTTTAGCTTGGGGGGGTGGGGGTGGGGTGGGGACTTGCAC TTATTCTCTAAAATGTTGACTAATCCAGAAAGCCTGATGCAACATAATCTGGAAAACTGCTTTGGTACAT TTGTAGAAATCCGTAGAAATACCATATCCAGCCTCAAAGCATTAATCTCAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC018548 unedited GGGGTTCACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCCTCGTG CCGAATTCGGCACGAGGAGGTGTGTGGAGGTCATTGCAAAGGAAGGACAAAACCTGAAAG AGCTATATTTGGTGTCCTGTAAAATCACAGATTATGCACTGATAGCCATTGGGCGATACA GCATGACAATAGAGACTGTGGATGTCGGATGGTGTAAAGAAATCACAGACCAAGGAGCCA CCCTGATTGCACAGAGCAGCAAGTCTCTGAGATATTTGGGGCTGATGAGATGTGATAAAG TCAACGAAGTGACGGTGGAACAGCTGGTGCAGCAGTACCCCCACATCACCTTCAGCACTG TCCTGCAGGACTGCAAGAGGACCTTGGAGAGAGCCTATCAGATGGGCTGGACCCCCAACA TGTCTGCCGCCTCCTCCTAGTGCTCCTGCCTCGCCTAGTCCACTGGGATCATTCAGCAGA GCAGAGGAGAATTGTACATTTGGGGAGCTGATCTCTCGGAAGGGTTTTAACTGTCACCTG TCTGTGTGTGTTCAAGTATGTGTATTTGTGTCTTGTTTGCATACTGCATACCAAAAGTGC AGTTGTCATTTGTTCCCAAGTGAGCTGGCTTTTTTTCTTAGAAATTAAAGATTATGAAAG CCACAAACTTTTTAGTTTTTAACTTCAAAGGCTTCTTTCTCTTTAAAAAAAAATTATTTT TAATATAGAGTCAAAAAATTGGATGTATTATTCTGAGCTTCTAATTGCTGCCACTTGGAG ATGTCCAAGTAAGAAGGCCTTCTTTNTACATGGATGAAATGTGCACTTCTACTGATGATG ACTACAGCAGAAGTGATGTTACACGTACAGGCACTTACACTCTCTCACATATTCACACGT ATGCACATCTACACCAGGNCTCTGATCACTNCTAGATCGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC018548 unedited TTTTTTTTTTTTTTTTTGAGATAATGCTTTGAGGCTGNGAATGGTATTTCTATGGATTTC TACAAATGTACCAAAGCAGTTTTCCAGATTATGTTGCATCAGGCTTTCTGGATTAGTCAA CATTTTAGAGAATAAGTGCAAGTCCCCACCCCACACCCAACCCCCCAAGCTAAAGCATGT TATAGTGCTGTACTTTAGATTCAAACAATGCAACATTTAAAAAAATCAATTATACATTCT GAAACCATTTGACAACCAAACTGTAACACTCCAAAGGTTATTACAACTTTGAACTGAAAT GGTACCATCTATATCATAGATGGCTCTCCCTTCTTTTGTCTTTTTTAACTATTTAAAATA TAGTATGACATATATAAAAATTAAACTTTTGCATATTCACTCAAGCTACTATTACTATAC ATACTGTCTTATAAATTAGTTTTGGATTAAAAATCAGTTAAAATATTGTGAAATAAGATA TTTCAGTGGTGGGGAAGTGGATATGAAAATGATACAGTTCAATGTGTAAGAAGAAATATA GTTTATTAATGTTAAACAGCTTTTTTCTTACACAAATTNTATTCCCTTCAAATTAAATTA GAAACCCATTTAGTTATGGGAAGAATACTCATGGTGCTTACTGACTAGATTTTCCTAACC GATTGGGATTAAAACAAAAATGCCACATCAGAAACATGTTGACATAATCAAAGGAAACAG TCGTTTACAAAAAAA
Restriction Sites	NotI-NotI
ACCN	BC018548
Insert Size	2770 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018548.1
RefSeq Size	2676 bp
RefSeq ORF	1515 bp
Locus ID	64839
Protein Families	Druggable Genome
Gene Summary	Members of the F-box protein family, such as FBXL17, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]

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