HSCB (BC000004) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DNAJC20; HSC20; JAC1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC000004, the custom clone sequence may differ by one or more nucleotides GGCACGAGGTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGC CAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAA GGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGCGG CCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCC ACTCGAGACTACTTCAGCCTTATGGACTGCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACA GGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTT CTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTG TACCTTCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATTCCTCA TAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATC CATTGTCAAAGCTAAACAGAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAA GAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAGTTAAAGAAGA TTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCT GTAATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCA ACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGGGCATGGTGGCGCGTGCCTGTAATCC CAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGAT CACGCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAA AAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC000004 unedited CGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACGGCTAGATAGGCCGCCGGCC AGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGG TTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCC GCTGTTGGAACTGCGGCGGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCAC AGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACTGCA ACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTC TTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTTCTCAGAGAAGC ATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGT ACCTTGTAAGCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGA CAGGCAATTCCTCATAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGA AGCTGCCATGAAAGAGATTGAATCCATTGTCAAAGCTAAACAGAAAGAATTTACTGACAA TGTGAGCAGTGCTTTTGAACAAGATGACTNTGAAGAAGCCAAGGAAATTTTGANCAAGAT GAGATACTTTTCAATATAGAAGAANAGATCAGTTAAAGAAGATTCCCCTTTAATTGTGGA TAGTTTAAAGTTTAAAAATAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAG CACTTTGGGAAGGCTGAGTGGGTGGATGACAAGGTCANGGAGTCAAGACCAGCTGGCACT TTAANTGAAAACCCGTCTCTGCTGAAATACAAAATAGCN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC000004 unedited CAATCCCCTGCACCGCGGCCCGCAATTTACAGGCGGTTTTTTTTTTTTTTTTTTCATTTA TTTACTTTTTAAGATTACCTCTCACTCTGTTGCCCAAGATGGAGTGCAGCTGCGTGACCT TTGATCACTGAAACCTCCACCTAACGGAATTAAGCGACTCTCCTGCCTCGCCCTACCACG AAGCTGGAAATACAGGCCCGCGCCACCATGCCCGGCTAATTTTTGCATTTTCAGCAGAGC ACGGAGTTAAACTATGCCGCCCAAGTTGGTCTTGCCCTCCCGACCTTGCCATCCACCCAC CTAAGCCTCCCACAGCGCTGGCACCACCGGTGTGAGCCACTTGTGCCCACGCAAGCACCT TATTTTTCTACCTTCTAACCTATTCCCACTTAAAGGGGACCTCTCTTTAACTTGATCTTT CCTTCTATATCTCGCACGCATCCCATCTTTCGCCACAACTCTTCCGCCTTTCTCACGTCC TCCCGCCCAAAAGCACTGCTCACATCGCCCCGACCATTCTTTTTGGTCAGCATCTGCAGC GAAACCAACCTCTTTCATGCTAGCTTCACCTCCACCTCTCGCGCGCTACTCCTCGCCTCC CCATACCCCAACCGAGGAACCCGCCCGCCAATCACCTAACCNGCACTTTAAGGCCACCTA CACTCTAGGACGTCTCTCACCCACCACCATCGATCTTCCGCAAACGTAGGCATCACTCGC CACCCACCCCTATTCCTCACCACCCTCGACTGATCTTTTCCCCATCCCTCTATGCTCCTA ACCCCCATACTTTACCCCTCCGTCCTCCTCTTCCACGCTCTTTCTTTCACTCCACCCTCT AACACTCCACTCTCAATCCCTTCCTATCCTTTCAAACATTCCATTCCTTCTTCCCCCCCC TCTCTCTCCTTTCTCTGCTATTTATTTCTCCATCCCAACCTCTCCCTCCTCATCCTC
Restriction Sites	NotI-NotI
ACCN	BC000004
Insert Size	1080 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC000004.1
RefSeq Size	1138 bp
RefSeq ORF	1138 bp
Locus ID	150274
Domains	DnaJ
Gene Summary	This gene encodes a DnaJ-type co-chaperone and member of the heat shock cognate B (HscB) family of proteins. The encoded protein plays a role in the synthesis of iron-sulfur clusters, protein cofactors that are involved in the redox reactions of mitochondrial electron transport and other processes. Cells in which this gene is knocked down exhibit reduced activity of iron-sulfur cluster-dependent enzymes including succinate dehydrogenase and aconitase. The encoded protein may stimulate the ATPase activity of the mitochondrial stress-70 protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

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