ANKHD1 (AK074173) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MASK; MASK1; PP2500; VBARP
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK074173, the custom clone sequence may differ by one or more nucleotides GCTGCTGGGACGGGGGAAAGGAGACGCTTCTTCCTCTTGCTGCTCTTCTCGTTCCCGAGATCAGCGGCGG CGGTGACCGCGAGTGGGTCGGCACCGTCTCCGGCTCCGGGTGCGAACAATGCTGACTGATAGCGGAGGCG GCGGCACCTCCTTTGAGGAGGACCTGGACTCTGTGGCTCCGCGATCCGCCCCAGCTGGGGCCTCGGAGCC GCCTCCGCCGGGAGGGGTCGGTCTGGGGATCCGCACCGTGAGGCTCTTTGGGGAGGCCGGGCCAGCGTCG GGAGTCGGCAGCAGCGGCGGCGGCGGCAGCGGCAGCGGTACGGGCGGAGGGGACGCGGCGCTGGATTTCA AGTTGGCGGCTGCCGTGCTGAGGACCGGGGGTGGAGGTGGTGCCTCTGGCAGTGACGAGGACGAAGTGTC CGAGGTTGAATCATTTATTTTGGACCAAGAAGATCTGGATAACCCAGTGCTTAAAACAACATCAGAGATA TTCTTATCAAGTACTGCAGAAGGAGCAGACTTACGCACTGTGGATCCAGAGACACAGGCACGACTAGAAG CATTGCTAGAAGCAGCAGGAATTGGCAAATTGTCAACTGCTGATGGTAAAGCTTTTGCAGATCCTGAGGT ACTCCGGAGACTGACATCCTCAGTTAGTTGTGCACTGGATGAAGCTGCTGCTGCACTGACACGGATGAAA GCAGAAAACAGCCACAATGCAGGACAAGTGGACACTCGCAGTCTAGCAGAAGCTTGTTCAGATGGGGATG TTAATGCTGTTCGTAAATTGCTAGATGAAGGCAGAAGTGTAAATGAACATACAGAAGAAGGAGAAAGCCT GCTGTGTTTGGCTTGTTCAGCAGGGTATTATGAATTAGCACAAGTATTGCTTGCTATGCATGCTAATGTT GAAGATCGAGGGAATAAAGGAGACATAACTCCCCTGATGGCAGCTTCCAGTGGAGGTTACTTAGATATTG TGAAATTATTACTTCTTCATGATGCTGATGTCAACTCCCAGTCTGCAACAGGAAACACTGCGCTAACTTA TGCATGTGCTGGAGGATTTGTTGACATTGTTAAAGTGCTCCTTAATGAAGGTGCAAATATAGAAGATCAT AATGAAAATGGACATACTCCCTTAATGGAAGCAGCCAGTGCAGGTCATGTGGAAGTTGCAAGAGTTCTTT TAGATCATGGTGCAGGCATCAACACTCATTCTAATGAATTCAAAGAAAGTGCTCTAACACTTGCTTGCTA CAAAGGCCATTTGGATATGGTTCGCTTTCTACTTGAAGCTGGTGCAGATCAAGAGCACAAAACAGATGAG ATGCACACTGCCTTAATGGAGGCCTGCATGGATGGACATGTAGAGGTGGCACGTTTGCTTTTGGATAGTG GTGCTCAAGTGAACATGCCTGCAGATTCATTTGAATCTCCATTGACGCTAGCTGCCTGTGGAGGACATGT TGAATTGGCAGCTCTACTTATTGAAAGGGGAGCAAATCTTGAAGAAGTTAATGATGAAGGATACACTCCC TTGATGGAAGCTGCCCGGGAAGGACATGAAGAAATGGTGGCACTACTCTTAGCACAAGGAGCAAATATAA ATGCCCAGACAGAAGAAACTCAAGAAACTGCTCTTACTTTGGCTTGCTGTGGAGGATTTTCTGAAGTTGC AGACTTTCTTATTAAGGCAGGGGCTGATATAGAACTTGGCTGCTCCACACCTCTGATGGAGGCATCTCAG GAGGGACACCTGGAATTGGTTAAATATTTGCTGGCTTCTGGCGCTAATGTGCATGCTACAACAGCAACAG GAGACACAGCCTTAACCTATGCTTGTGAAAATGGACATACGGATGTTGCAGATGTTTTACTTCAAGCAGG GGCTGATTTAGACAAGCAGGAGGACATGAAGACTATTTTGGAGGGCATAGATCCGGCCAAGCATCAGGAA CATGAATCTGAAGGTGGAAGAACACCTTTGATGAAAGCTGCAAGAGCTGGTCATTTGTGCACTGTGCAGT TTCTTATTAGCAAAGGTGCCAATGTTAACAGGGCTACAGCCAATAATGATCATACAGTAGTGTCGCTGGC ATGTGCAGGAGGCCACCTGGCAGTTGTTGAGCTTCTCTTGGCTCATGGGGCTGACCCTACTCATCGACTC AAGGATGGTTCAACAATGCTCATTGAAGCTGCAAAGGGTGGCCATACTAATGTAGTTTCTTATCTGTTGG ATTATCCAAATAATGTTCTGTCAGTTCCCACCACAGATGTGTCTCAGCTCCCTCCACCTTCTCAAGATCA GTCTCAGGTGCCACGTGTGCCAACGCATACACTTGCCATGGTTGTACCTCCCCAGGAACCTGACAGAACT TCACAGGAGAACTCTCCTGCCCTTTTAGGAGTGCAAAAAGGTACATCCAAGCAGAAGTCCAGTTCCCTCC AGGTAGCAGATCAGGACCTACTGCCATCTTTTCACCCATACCAGCCTTTGGAGTGCATAGTAGAGGAGAC TGAAGGCAAGCTGAATGAACTGGGACAAAGAATTAGTGCTATTGAAAAAGCACAGCTTAAGTCACTGGAG TTAATTCAAGGTGAACCTCTGAACAAAGATAAGATAGAAGAACTTAAAAAGAACAGAGAAGAGCAAGTCC AGAAGAAGAAGAAAATATTGAAAGAACTGCAGAAAGTGGAAAGGCAGTTGCAGATGAAAACACAGCAGCA ATTTACCAAAGAATACTTGGAAACCAAAGGTCAGAAAGACACAGTGTCTCTACACCAACAGTGCTCTCAT AGAGGAGTCTTCCCAGAAGGGGAAGGAGATGGTAGTCTCCCAGAGGATCACTTTTCAGAGTTACCTCAGG TTGACACAATCTTATTTAAAGATAATGATGTTGATGATGAGCAACAGTCTCCACCATCGGCAGAACAGAT TGATTTTGTCCCAGTCCAGCCTTTATCATCTCCACAGTGTAACTTTTCCAGTGACTTAGGTTCTAATGGG ACAAATTCTCTTGAACTTCAGAAAGTATCAGGTAATCAGCAGATTGTAGGACAGCCTCAGATTGCTATTA CTGGACATGATCAGGGGCTGTTAGTTCAAGAACCAGATGGACTAATGGTTGCAACTCCAGCTCAGACGCT TACCGACACTCTTGATGACCTGATAGCAGCTGTGAGTACCAGAGTGCCCACTGGTTCCAACAGTTCTTCT CAGACCACAGAGTGTCTTACACCTGAATCCTGTTCGCAGACTACAAGCAATGTGGCTTCCCAATCGATGC CTCCTGTGTATCCTTCAGTTGACATTGATGCACATACTGAGAGCAATCATGACACAGCATTAACACTAGC TTGTGCAGGTGGTCATGAAGAACTTGTATCTGTGCTCATTGCACGGGATGCCAAAATTGAACACAGAGAC AAAAAAGGTTTCACACCACTAATCCTGGCAGCAACAGCAGGGCATGTTGGAGTTGTTGAAATCCTTTTGG ATAAAGGTGGAGATATAGAAGCACAGTCTGAACGAACTAAGGATACTCCGCTTTCATTGGCATGTTCTGG TGGACGTCAGGAGGTGGTAGACTTGCTGCTGGCTCGAGGTGCAAATAAAGAACATAGGAACGTATCTGAT TATACACCACTGAGTCTAGCTGCGTCTGGAGGATATGTTAATATCATTAAGATTCTGCTTAATGCTGGGG CAGAAATTAATTCAAGGACTGGGAGTAAACTAGGTATTTCTCCCCTGATGTTGGCTGCAATGAATGGACA TGTTCCTGCAGTAAAATTGCTGCTCGATATGGGTTCAGACATTAATGCCCAAATAGAGACCAATCGGAAC ACGGCTCTCACCCTGGCCTGTTTCCAGGGCCGAGCAGAAGTAGTGAGTTTGCTTCTGGACCGAAAAGCCA ATGTTGAACATAGGGCAAAGACGGGTCTTACCCCCTTGATGGAAGCAGCTTCTGGAGGGTATGCAGAGGT TGGAAGAGTTCTTCTTGATAAAGGAGCAGATGTTAATGCTCCCCCTGTGCCTTCCTCAAGAGATACTGCT TTAACAATAGCAGCAGACAAAGGTCACTACAAATTTTGTGAACTCCTGATTCATAGGGGAGCCCACATTG ATGTTCGTAACAAAAAGGGAAATACGCCACTTTGGCTGGCATCCAATGGAGGTCATTTTGATGTTGTGCA GTTGCTAGTGCAAGCAGGTGCTGATGTGGATGCAGCAGATAACCGGAAAATCACACCTCTTATGTCAGCA TTTCGCAAGGGTCATGTAAAAGTTGTTCAATATTTGGTAAAGGAAGTAAATCAGTTCCCTTCTGATATAG AATGCATGAGATACATAGCAACAATTACAGATAAGGAACTGTTGAAAAAATGTCATCAATGTGTCGAAAC CATTGTGAAGGCTAAAGACCAGCAAGCTGCAGAAGCAAATAAGAATGCGAGTATTCTTTTAAAGGAACTT GATCTGGAAAAGTCAAGAGAAGAGAGCAGAAAGCAGGCTCTTGCTGCTAAAAGAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK074173 unedited CTAAACTTTAAACCCCCCGTTGCAGCCAAAGGGCGGTAGGCGTGTACGGTNGGGAGGTCT ATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGC GGCCGCGAATTCGGCACCAGACGGGGGAAAGGAGACGCTTCTTCCTCTTGCTGCTCTTCT CGTTCCCGAGATCAGCGGCGGCGGTGACCGCGAGTGGGTCGGCACCGTCTCCGGCTCCGG GTGCGAACAATGCTGACTGATAGCGGAGGCGGCGGCACCTCCTTTGAGGAGGACCTGGAC TCTGTGGCTCCGCGATCCGCCCCAGCTGGGGCCTCGGAGCCGCCTCCGCCGGGAGGGGTC GGTCTGGGGATCCGCACCGTGAGGCTCTTTGGGGAGGCCGGGCCAGCGTCGGGAGTCGGC AGCAGCGGCGGCGGCGGCAGCGGCAGCGGTACGGGCGGAGGGGACGCGGCGCTGGATTTC AAGTTGGCGGCTGCCGTGCTGAGGACCGGGGGTGGAGGTGGTGCCCTCTGCAGTGACGAG GACGAAGTGTCCGAGGTTTGATCATTTATTTTTGACCCAGAAGATCTGGATNACCCAGTG CTTAANACACATCAGAGATATTCTTTATCAGTACTGCAGAAGGAGCAGACTTACGCACTG TGGATCCCAGAGACACAGCACGACTAGNAGCATTGCTAGNAGCAGCAGGAATTGNCAAAT TGTCAACTGCTGATGGTAAAAGCTTTGCAGATCCCTGAGTACTNCNGAGACTGACATCTC AGTTATTGTGCACTGGATGAAGCTGCTGCTGCACTGACACGGATGAAAGCGAANACAGCC ACNATGCAGGGACAGTGGACACTCGCAGTCTAGCAGAAGCTTGTTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK074173 unedited CCCTAGGTTGCCCGCGGCCGCAATCTACGAGCGAGNTTTTTTTTTTTTTTTTTCTTTTCT TTTTTCTCTTTTAGCAGCAAGAGCCTGCTTTCTGCTCTCTTCTCTTGACTTTTCCAGATC AAGTTCCTTTAAAAGAATACTCGCATTCTTATTTGCTTCTGCAGCTTGCTGGTCTTTAGC CTTCACAATGGTTTCGACACATTGATGACATTTTTTCAACAGTTCCTTATCTGTAATTGT TGCTATGTATCTCATGCATTCTATATCAGAAGGGAACTGATTTACTTCCTTTACCAAATA TTGAACAACTTTTACATGACCCTTGCGAAATGCTGACATAAGAGGTGTGATTTTCCGGTT ATCTGCTGCATCCACATCAGCACCTGCTTGCACTAGCAACTGCACAACATCAAAATGACC TCCATTGGATGCCAGCCAAAGTGGCGTATTTCCCTTTTTGTTACGAACATCAATGTGGGC TCCCCTATGAATCAGGAGTTCACAAAATTTGTAGTGACCTTTGTCTGCTGCTATTGTTAA AGCAGTATCTCTTGAGGAAGGCACAGGGGGAGCATTAACATCTGCTCCTTTATCAAGAAG AACTCTTCCAACCTCTGCATACCCTCCAGAAGCTGCTTCCATCAAGGGGGTAAGACCCGT CTTTGCCCTATGTTCAACATTGGCTTTTCGGTCCAGAAGCAAACTCACTACTTNTGCTCN GCCCTGGAAACACGCCAGGGTGAGAGCCGTGTTCCCGAATGGCCTCTATTTTGGCCATAA TGGTCTGAACCCATATCGAGCAGCCATTTTACTGCANGAACATGTCCATTCATTGCAGCC ACATCAGGGAAGACAACTAAGTTACTCCCAACCCTGGATTAATTCTGCCCAGCATAAGCC CAACTTTAGGATTACCCATCCTTCGACCC
Restriction Sites	NotI-NotI
ACCN	AK074173
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK074173.1, BAB84999.1
RefSeq Size	4535 bp
RefSeq ORF	4535 bp
Locus ID	54882
Domains	ANK
Gene Summary	This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. The protein is thought to function as a scaffolding protein, and it may be involved in the regulation of caspases and thereby play an antiapoptotic role in cell survival. Alternative splicing results in multiple transcript variants, one of which generates a fusion transcript (MASK-BP3) with the downstream eIF4E-binding protein 3 (EIF4EBP3) gene, resulting in a protein comprised of the ANKHD1 sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq, Sep 2010]

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