WDR55 (AK025355) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105321 sequence for AK025355 edited (data generated by NextGen Sequencing) ATGGACCGCACTTGTGAGGAGAGGCCCGCTGAGGATGGGAGCGACGAGGAGGACCCAGAC TCCATGGAAGCCCCAACCCGGATCCGGGACACTCCGGAAGACATCGTGCTGGAAGCTCCG GCTAGTGGGCTGGCGTTCCATCCGGCCCGTGACCTACTGGCTGCAGGGGACGTGGACGGG GACGTGTTCGTCTTTTCCTACTCTTGCCAAGAGGGAGAAACCAAGGAGCTCTGGTCATCA GGTCACCATCTCAAGGCCTGCCGAGCTGTGGCCTTCTCTGAAGATGGGCAGAAGCTCATT ACTGTCTCCAAGGACAAAGCCATCCATGTTCTAGATGTGGAGCAGGGCCAACTGGAAAGA CGTGTTTCCAAGGCTCATGGTGCCCCCATCAATAGTCTTCTGCTGGTGGATGAGAATGTT CTGGCCACTGGGGATGACACAGGTGGTATCCGTCTCTGGGACCAGCGGAAGGAGGGCCCC TTAATGGATATGAGGCAACATGAAGAGTACATCGCAGACATGGCTCTGGATCCAGCCAAA AAGCTGCTGCTGACAGCCAGCGGGGATGGCTGCCTTGGCATCTTCAACATTAAGAGGCGT CGGTTTGAGCTGCTCTCAGAACCTCAGTCTGGGGACCTGACCTCTGTCACTCTCATGAAA TGGGGGAAGAAGGTAGCCTGTGGCTCCAGTGAAGGTACCATCTACCTCTTCAATTGGAAT GGCTTTGGGGCCACAAGTGACCGCTTTGCCCTGAGAGCTGAATCTATCGACTGCATGGTT CCAGTCACCGAGAGTCTGCTGTGTACTGGCTCCACTGATGGAGTCATCAGGGCTGTGAAC ATCCTACCGAACCGAGTGGTGGGCAGTGTGGGCCAGCACACTGGGGAGCCTGTGGAGGAG CTGGCCCTCTCCCACTGTGGCCGCTTCCTGGCCAGTAGTGGCCATGACCAGCGCCTCAAG TTTTGGGACATGGCCCAGCTGCGAGCTGTGGTGGTGGATGACTACCGTCGGCGCAAAAAA AAGGGAGGACCACTGCGGGCTCTGAGCAGCAAGACTTGGAGCACCGATGACTTCTTCGCA GGACTGAGGGAAGAGGGAGAAGACTCCATGGCTCAGGAAGAAAAGGAGGAGACTGGGGAT GACAGTGACTGA Clone variation with respect to AK025355.1 393 c=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK025355 unedited GTCTTACCCCGCCCGTTCNCGCTAATGGGCGGTAGCGTGTACGGTGGNGAGTCTATATAA GCAGTAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC GCGAATTCGGCACGAGGGGCTGACTACCCTGCGGCGGCGCGGCTCGCAGTCCTTCTCAGC ATGGACCGCACTTGTGAGGAGAGGCCCGCTGAGGATGGGAGCGACGAGGAGGACCCAGAC TCCATGGAAGCCCCAACCCGGATCCGGGACACTCCGGAAGACATCGTGCTGGAAGCTCCG GCTAGTGGGCTGGCGTTCCATCCGGCCCGTGACCTACTGGCTGCAGGGGACGTGGACGGG GACGTGTTCGTCTTTTCCTACTCTTGCCAAGAGGGAGAAACCAAGGAGCTCTGGTCATCA GGTCACCATCTCAAGGCCTGCCGAGCTGTGGCCTTCTCTGAAGATGGGCAGAAGCTCATT ACTGTCTCCAAGGACAAAGCCATCCATGTTCTAGATGTGGAGCAGGGCCAACTGGAAAGA CGTGTTTCCAAGGCTCATGGTGCCCCCATCAATAGTCTTCTGCTGGTGGATGAGAATGTT CTGGCCACTGGGGATGACACAGGTGGTATCCGTCTCTGGGACCAGCGGAAGGAGGGCCCC TTAATGGATATGAAGGCACATGAAGAGTACATCGCAGACATGGCTCTGGATCCAGCCAAA NAGCTGCTGCTGACAGCCAGCGGNGATGGCTGCCTTGGGCATCTTCACATTAAGAGGCGT CNGTTTGAGCTGCTCTCAGAACCTCAGTCTGGGGACCTGACCTCTGTCACTCTCATGAAA TGGGGGAAAGAAGTAGCCTGTGGCTCCAGTGAAGGTACATCTACCTCTTCAATGGAATGG CTTTGGGGCCCCAATGACCGCCTTGCCTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK025355 unedited GTGTACCGTGTGCCGCAATCNANAGTCGAGTTTTTTTTTTTTTTTTTTTGTCCTCTTTGT TGGTTTTTATTTGTGGGGAAGGGCAGCTGAGCCAAAGGGTCAGAAATTCTGCCCTTTGCC TCCACCACATGGCATTCTGGTTTTGGTTTCTGTATAGTTTTGGGTCTTTCTATGCTGGTT GTATTTATATTAAACCCCTGGTTAGTATATACTTGTGTCTGTGCTGTCTTGCCCTAGCTT AGAGTTTGTTTACACTGGTAGAGTGAATAGCTGCTACGGGGCAAGTACTTCCTCACATCA GTGCTTTGAGTGCATCTTCTTGTGTTGCACTGTAGGGTATTATAAATTCATATCCAGGCC GGGTGTGTTGGCTTTTGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGTGGATCAC CTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAGCATGGAGAAACCCCGTCTCTACTGAAA ATACAAAAATTAGCCTGGCATGGTGGCAGACACCTGTAATCCCAGCTACTTGGGAGGCTG AGGCAGGAGAATTGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGATCACGCCAT TGCACTCCAGCCCTGGCAACAAGAGCGAAACCCTGTCTCAAAAAAAAAAAAAAAAAAAAT TTCATATCCAGCCAGGCACAGTGGCTCATGCCTGTAATCCCAGCATTTTGGGAGGCCAAG GCAGGAGGATCACTTGAGCCCAGGAGTTCCAAGACCAGCTTGGTCAACATAGTGAAACCC CTGGTCTCTACCAAAAAAAAATCACANAAATTANTCAAGCATGGTGGCAGATGCCTGTTA AGTCTCAGCTACCANGCANGGCTGAGTGGATCACTTGAGCCCAGAAGTCANGGTGCAGTG ACCATGATCACCCACTGTCTTCANCCTGGTGCAGATGAGACCTGTCTAAAAATTAATTTT TTGGCAGTGTGTGCTCGGCCTTT
Restriction Sites	NotI-NotI
ACCN	AK025355
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK025355.1, BAB15118.1
RefSeq Size	2544 bp
RefSeq ORF	2544 bp
Locus ID	54853
Domains	WD40
Gene Summary	Nucleolar protein that acts as a modulator of rRNA synthesis. Plays a central role during organogenesis (By similarity).[UniProtKB/Swiss-Prot Function]

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