SHTN1 (AK095703) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KIAA1598
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105196 sequence for AK095703 edited (data generated by NextGen Sequencing) ATGAACAGCTCGGACGAAGAGAAGCAGCTGCAGCTCATTACCAGTCTGAAGGAGCAAGCA ATAGGCGAATATGAAGACCTTAGAGCAGAGAACCAGAAAACAAAGGAGAAGTGTGACAAA ATTAGGCAAGAACGAGATGAAGCCGTTAAAAAACTGGAAGAATTTCAGAAAATTTCTCAC ATGGTCATAGAGGAAGTTAATTTCATGCAGAACCATCTTGAAATAGAGAAGACTTGTCGA GAAAGTGCTGAAGCTTTGGCAACAAAGCTAAATAAAGAAAATAAAACGTTGAAAAGAATC AGCATGTTGTACATGGCCAAGCTGGGACCAGATGTAATAACTGAAGAGATAAACATTGAT GATGAAGATTCGACTACAGACACAGACGGTGCCGCCGAGACTTGTGTCTCAGTACAGTGT CAGAAGCAAATTAAAGAACTTCGAGATCAAATTGTATCTGTTCAGGAGGAAAAGAAGATT TTAGCCATTGAGCTGGAAAATCTCAAGAGCAAACTCGTAGAAGTAATTGAAGAAGTAAAT AAAGTTAAACAAGAAAAGACTGTTTTAAATTCAGAAGTTCTTGAACAGAGAAAAGTCTTA GAAAAATGCAATAGAGTGTCCATGTTAGCTGTAGAAGAGTATGAGGAGATGCAAGTAAAC CTGGAGCTGGAGAAGGACCTTCGAAAGAAAGCAGAGTCATTTGCACAAGAGATGTTCATT GAGCAAAACAAGCTAAAGAGACAAAGCCACCTTCTGCTGCAGAGCTCCATCCCTGATCAG CAGCTTTTGAAAGCTTTAGACGAAAATGCAAAACTCACCCAGCAACTTGAAGAAGAGAGA ATTCAGCATCAACAAAAGGTCAAAGAATTAGAAGAGCAACTAGAAAATGAAACACTCCAC AAAGAAATACACAACCTCAAACAGCAACTGGAGCTTCTAGAGGAAGATAAAAAGGAATTG GAATTGAAATATCAGAATTCTGAAGAGAAAGCCAGAAATTTAAAGCACTCTGTTGATGAA CTCCAGAAACGAGTGAACCAGTCTGAGAATTCAGTACCTCCACCACCTCCTCCTCCACCA CCACTTCCCCCTCCACCTCCCAATCCTATCCGATCCCTCATGTCCATGATCCGGAAACGA TCCCACCCCAGTGGCAGTGGTGCTAAGAAAGAAAAGGCAACTCAACCAGAAACAACTGAA GAAGTCACAGATCTAAAGAGGCAAGCAGTTGAAGAGATGATGGATAGAATTAAAAAGGGA GTTCATCTTAGACCCGTTAATCAGACAGCCAGACCGAAGACAAAGCCAGAATCTTCGAAA GGCTGCGAAAGTGCAGTGGATGAACTAAAAGGAATACTGGCCTCCCAGTAG Clone variation with respect to AK095703.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK095703 unedited GTAAAGTCAAATTTGTAAACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCAGCC ACAGGCTGGCATAGCGGCTGCCGACCCGCCCTCGTTCCTCCACCCCCTGAACGGGACTGC TGGGCCCGCCCCGCCCCGCCTGCAGGTGAAGCGGCCGCAGCCGCCGAGTAGGTGCGTGGG GATGATCTCACTCGCGCGCTCCGCGCCAGGAGGAGGAGGAGCGGGAGCGGATCCAACTTC CGGGTAGTGGAGCCGCAAGCCACCGGCATCTTGCTTTTTCTTCCCCCTCCTCCTGTGTGC CCCGCGCCGCTCCCTCTTTCCCTTTTATTCCCGGCCCCACCCGCCAAAATGAACAGCTCG GACGAAGAGAAGCAGCTGCAGCTCATTACCAGTCTGAAGGAGCAAGCAATAGGCGAATAT GAAGACCTTAGAGCAGAGAACCAGAAAACAAAGGAGAAGTGTGACAAAATTAGGCAAGAA CGAGATGAAGCCGTTAAAAAACTGGAAGAATTTCAGAAAATTTCTCACATGGTCATAGAG GAAGTTAATTTCATGCAGAACCATCTTGAAATAGAGAAGACTTGTCGAGAAAGTGCTGAA GCTTTGGCAACAAAGCTAAATAAAGAAAATAAAACGTTGAAAAGAATCAGCATGTTGTAC ATGGCCAAGCTGGGACCAGATGTAATAACTGAAGAGATAAACATTGATGATGAAGATTCG ACTACAGACACAGACGGTGCCGCCGAGACTTGTGTCTCAGTACAGTGTCAGAAGCAAATT AAAGAACTTCGAGATCAAATTGTATCTGTTCAGGAGGAAAAGAAGATTTTAGCCATTGAG CTGGAAAAATCTCAGAGCAACTCGTAGAAGTATTGGAGAAGTAATNAAGTAAACAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK095703 unedited ACTATGNNACCGCGGCCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGGGAAACT ATTGGTGCCTTTTTAATAAGTTGTGGTGAGGAGAGTTCAAATTACAGCATAACAGAATTC GTTTCGTGAATTAATGCAGTCTGTAGTAAGCATATCATATAGCAGTATTATCCAGTTAAA GAAAGATACAGTTGAAAAACATTACGTTTTAATTCTCCATGAGTAAAGTGATAAGTAACT ATAAAATCATTATTGGGAGAACATGGAAACAGTCAAGCATAACGAACTTACAGAAAGATA ATTATCTCCAAATTTAGGAAGTACATGTACCTGCCTACCCACCTCTTCAAGCCTATGCTT ACCACACGTGCAAAAATACAATACAATACAACTACTGCAATTATTACTATCATTTTCTTT TGCCCTTAGGTGAAAAACACCTGACAGCTACATGCTGAGCCATGCTAACAAAACTAAACC TTTCACTTTCTTTAATAGTAAAATTACCATTACTGAATCATTGTCATAAAAAGTGCATTC AAGTACATTACCACTTATTTTAAATAAACACCAATTTTGAAACAATCAACTTTGAAAAGC TGCATAAGTTTTTTTTTTAATCCCTGATTACATTTCTATTTATTCACTGTGGTAGCCTGA AACATTGTATTTTTAGAGACTAACTACTGCTTAATTTCTTTTTTAAAAAAAACAACACCA ACCTGTGTAATATAGAACAGCAGTGAAGCAGGAAAAAGTGTGCTTGTCACCTATCTTCAC CATACTGTACATAAGAACTCTGATNAAAAACGGGACCTGTGGCCCANTAGTATTTGGGAA AACTAATTAACTCCTAGCCCAGAGCCCATGGTTTACCAACCTACTACTGTAAAATTCAGG TTTGGGACTTTTACCCCATTCTGGTTTCAG
Restriction Sites	NotI-NotI
ACCN	AK095703
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK095703.1, BAG53109.1
RefSeq Size	3417 bp
RefSeq ORF	3417 bp
Locus ID	57698
Gene Summary	Involved in the generation of internal asymmetric signals required for neuronal polarization and neurite outgrowth. Mediates netrin-1-induced F-actin-substrate coupling or 'clutch engagement' within the axon growth cone through activation of CDC42, RAC1 and PAK1-dependent signaling pathway, thereby converting the F-actin retrograde flow into traction forces, concomitantly with filopodium extension and axon outgrowth. Plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. Plays also a role in regenerative neurite outgrowth. In the developing cortex, cooperates with KIF20B to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in the accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the growth cone of primary hippocampal neurons.[UniProtKB/Swiss-Prot Function]

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