COQ10A (AK057003) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105037 sequence for AK057003 edited (data generated by NextGen Sequencing) ATGGCCTGGGCGGGCTCGCGGCGGGTCCCAGCTGGGACGCGCGCGGCAGCCGAGCGCTGC TGCCGGCTCTCGCTCAGCCCGGGCGCGCAACCGGCCCCGCCCCCAGGCCCTCTGCCACCG CCGCGACCAATGAGGTTTCTGACCTCCTGCAGCCTCCTCTTGCCTCGGGCTGCCCAGATC TTGGCGGCTGAGGCTGGCTTACCTTCGAGCCGTTCCTTCATGGGATTTGCTGCTCCCTTC ACCAACAAGCGAAAGGCTTACTCGGAGCGTAGAATCATGGGGTACTCAATGCAGGAGATG TATGAGGTGGTGTCCAACGTCCAGGAGTATCGTGAGTTTGTGCCCTGGTGTAAGAAGTCT CTGGTGGTATCCAGCCGTAAGGGTCATTTGAAAGCCCAGCTGGAGGTTGGCTTTCCACCT GTCATGGAACGTTACACCTCTGCAGTTTCCATGGTCAAACCTCACATGGTCAAGGCTGTT TGTACTGATGGCAAGCTCTTCAACCACTTAGAGACTATTTGGCGATTCAGCCCTGGTATT CCTGCCTATCCTCGAACCTGCACTGTGGACTTTTCGATTTCCTTTGAATTTCGTTCTCTG CTGCACTCCCAGCTGGCCACCATGTTTTTTGATGAGGTTGTCAAACAGAATGTTGCTGCC TTTGAGCGTCGGGCAGCCACCAAGTTTGGTCCAGAAACAGCCATCCCCCGTGAACTGATG TTCCATGAGGTGCACCAGACTTGA Clone variation with respect to AK057003.1 113 c=>t;605 g=>a;638 c=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK057003 unedited AATTTGGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGAGGGCGCAGCCC GCGTCAGAACTTAGAGGGCCAGGCAGGGTCGCGCGCATGGCCTGGGCGGGCTCGCGGCGG GTCCCAGCTGGGACGCGCGCGGCAGCCGAGCGCTGCTGCCGGCTCTCGCTCAGCCCGGGC GCGCAACCGGCCCCGCCCCCAGGCCCTCTGCCACCGCCGCGACCAATGAGGTTTCTGACC TCCTGCAGCCTCCTCTTGCCTCGGGCTGCCCAGATCTTGGCGGCTGAGGCTGGCTTACCT TCGAGCCGTTCCTTCATGGGATTTGCTGCTCCCTTCACCAACAAGCGAAAGGCTTACTCG GAGCGTAGAATCATGGGGTACTCAATGCAGGAGATGTATGAGGTGGTGTCCAACGTCCAG GAGTATCGTGAGTTTGTGCCCTGGTGTAAGAAGTCTCTGGTGGTATCCAGCCGTAAGGGT CATTTGAAAGCCCAGCTGGAGGTTGGCTTTCCACCTGTCATGGAACGTTACACCTCTGCA GTTTCCATGGTCAAACCTCACATGGTCAAGGGCTGTTTGTACTGATGGCAAGCTCTTCAA CCACTTAGAGACTATTTGGCGATTCAGCCCTGGTATTCCTGCCTATCCTCGAACCTGCAC TGTGGACTTTTCGATTTCCTTTGAATTTCGTTCTCTGCTGCACTCCCAGCTGGCCCCATG GTTTTTGGATGAAGGNTGTCAAACAGAATGTTGCTGCCTTTGAGCGTCGGCAGNCCCAAA GTTTGGGTCAGAACAGCCATCCCCGTGAACTGNATGTCCATGAGGTGACCAGACTTGAGC AAGGGNATGCTCCCTGACCTCCCTCTACCCACTTCCTACACATTCTATTTATTTTGGTTG GCCTCTGGTCATTGGAAGAAANCTGGTCATATACTGTTCTCCTTCATTCCAAAAAATGGG TCCTGCTGCTGAAATGTGGGGGAAAGAAAGCCAGTAGGAAAAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK057003 unedited NCCGTCTACTCAGNACGCGCCGCTTTCTANGATCGGTTTTTTTTTTTTTTTTTTAACACT GTCACAATTTATTGAAATTTTATAAAAACTCAGGCCAAGTGGAAGAATAAGGTACAACTC AAGAGTACAAAGACAACTCCGTTTCCGTTCAGTACTTTTCTCCTCAGCACTGGTGGTAAG AAAGCCCCTTGCTCTCTAGTAGCCAGGCAGCATGGACTTACAGTCTTAAAATGAGGCTTT ATGTATTTCAGGCTGGAGGCAGGTTGCCTTTTCTCCTGAGGAATCTCAGGCAGGGTAAAA GTTACTTACCACTCAGTACCTCTGTGCCAGAAGAAAAGCTCAATTTATTCAATCCTTAGA AAAGTTACTATCGTCCCCTGGTCAGACACTAAGGTGTCTTGATAAGGTCCAGAGACAACC ACATAGTCCTTATTCTAATTCGTAGTGAAAAGGCTGCAGGCATATGGTGCTCCTACGATG GGCCTGACCCTTTCCTAAGCACATCTCATTTCCACATCCTTTGCCTTCTCTTTCCCCCAA CATTTCCAGCCAGCATAGAACCCAATTTCTGGGAAATTGAGAGGAGAAACAGTNTTATGA ACACAGACTCCTTTCAAATTGGAACAGGAGCCAAACCAAATAAATAAGAGAATTGTGTAG GGAAGTGGGGTAGAAAGGAGGTCAGGGAGCAATCCCTTGCCTCAAGTCTGGTGCACCTCA TGGAACATCAGTTCACGGGGGATGGCTGTTTCTGGGACAAACTTGGTGGCTGCCCGACGC TCATAGCAACAACATTCTGTTTGACACCCTCTCAAAAAACTGGTGGCCAGCTTGGAATGC AGCAGAAAACAAAT
Restriction Sites	NotI-NotI
ACCN	AK057003
Insert Size	1600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK057003.1, BAB71339.1
RefSeq Size	1568 bp
RefSeq ORF	744 bp
Locus ID	93058
Domains	ARPF
Gene Summary	Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes (Probable).[UniProtKB/Swiss-Prot Function]

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