NBR2 (BC022065) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NCRNA00192
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC022065, the custom clone sequence may differ by one or more nucleotides AGCGGGGTTGCGGCTTATTGCATCACAGTAATTGCTGTACGAAGGTCAGAATCGCTACCTATTGTCCAAA GCAGTCGTAAGAAGAGGTCCCAATCCCCCACTCTTTCCGCCCTAATGGAGGTCTCCAGTTTCGGTAAAAG CTTCATTTGATCTGAATAGTATTAAAATAAAATACCTGGATGAGGAAGATGAAGAGAGATGAAGTCTCGC TCTGTCACCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGCAACCTCTGCCTCCGGGTTCAAGCAA TTCTCCTGCTTCAGCCTCCCGAGTAGCTTGGGACTACAGACTAGAGACGGGGTTTCACTGTGTTAGCCAG GATGGTCTCGATCCCCTGACATCGTGATCCGCTGTCCTCGGTCTCCCAAAGTGCTGGGATTACAGGCGTG AGCCACCGCACCTGGTCGATTAACCTCTTTTAAGCCTGTTTTCTTATCCATACAATGGAGATAATGGCAG CACCCACTGCTTGACTGGGCAACAAGAGCGAAACTCCATCTCAAAAGGAAAAAAAAGCAAAAGAGAAAAA ACCCAAACAAACAAAACCCCTGAACTTCCCTGCGGGCACATTTTCTCTGGCAGCCTTTTTCAATGAGAGC TATGTTTTCTCCGATACTATATGGCCTGCGGACCGCTCAGCTTTCATTCCAGTGAAAACATTCCAGAACA AACTCCAGGTGCTGGAAGCCCAGGAAGCACACATCAAGGCTCCCTTGCCAGCAGGGTGCTGCCAATAAAA GGTAGTCACGTGGAATTTGGAATGTGGAAAGGAGGTAGAAGTCATCCTTTCCTCCCCTGTAGCAGCAGGC GTGCAGGCTCTGGTGGTCAGCTGGACTCCATACTCCCCCACCAGTCACCAGCCTGGGGACCGTGGGGCTG CAAGGACCTCAGCAGCGGTGTCCCAAGTTTCCTGACTTCTTCCATCCTCTGGAAATCAGCTGTGCCGGAC AGAGGAATGGTTACAAGGCTTTGAGCCTAACAGTGCTGGCTTAAAACCGTAACTTGTCGTTTTGGATGTG TGGCCTTGGACACTTGGGCAGGTCACCTACGTCCTTCTAGCCCTGGCTCAGTCCTTTGGATGTTAATCCA CCTGGGCTGGTGCACCTAAATAATAAAGATCCTCCTCAATCCCAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC022065 unedited AGAGCCAGAATTGTATACGACTCACTATAGGCGGCACGCGAATTCGCACCAGCCTGCCCT CTACCTCTACTCTTCCAGTTGCGGCTTATTGCATCACAGTAATTGCTGTACGAAGGTCAG AATCGCTACCTATTGTCCAAAGCAGTCGTAAGAAGAGGTCCCAATCCCCCACTCTTTCCG CCCTAATGGAGGTCTCCAGTTTCGGTAAAAGTTTCATTTGATCTGAATAGTATTAAAATA AAATACCTGGATGAGGAAGATGAAGAGAGACGAAGTCTCGCTCTGTCACCAGGCTGGAGT GCAGTGGCGTGATCTTGGCTCACTGCAACCTCTGCCTCCGGGTTCAAGCAATTCTCCTGC TTCAGCCTCCCGAGTAGCTTGGGACTACAGACGTGCGCAACCATGCCCAGCTAATTCTTT TTTTCTTTATTTTATTTTATTTTATTTTTTGAGATGGAGTCTCGCTCTGTCGCCCAGGCT GGAGTGTAGTGGCGCGATCTTGGCTCACTGCAAGCTCTGCCTCCCGGGTTCACGCCATTC TCCTGCCTCAGCTTCCTGAGTAGCTTGGACTACAGGCGCCCACCAGCACGCCCAGCTAAT TTTTTTTTTTCTTTGTATTTTTAGTAGAGACGGGGTTTCACTGTGTTAGCCAGGATGGTC TCGATCCCCTGACATCGTGATCCGCTGTCCTCGGTCTCCCAAAGTGCTGGGATTACAGGC GTGAGCCACCGCACCTGGTCGATTAACCTCTTTTAAGCCTGTTTTCTCATCCATACAATG GAGATTATGGCAGCACCCACTGGCTGNTAAGGAATATTANGTGAACACAGGTGCCTATGT GGGTTTCCTCCTTTTTCATAAATGGTTATTTCCCTTATCCCTCATCTCCTAAGTTAGTCC CA
Restriction Sites	NotI-NotI
ACCN	BC022065
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC022065.1
RefSeq Size	1179 bp
RefSeq ORF	1179 bp
Locus ID	10230
Gene Summary	This gene was identified by its close proximity on chromosome 17 to tumor suppressor gene BRCA1. Experimental evidence indicates that the two genes share a bi-directional promoter. Transcription for either gene is controlled individually by distinct transcriptional repressor factors. A short (112 amino acid) open reading frame is observed which includes a region derived from a LINE1 element. A strong Kozak signal is not observed for the putative ORF and the stop codon is more than 55 nucleotides upstream of the last splice site for the transcript, suggesting that the transcript is subject to nonsense-mediated decay. Therefore, this gene does not appear to encode a protein. Glucose starvation induces the expression of this gene and the long non-coding RNA transcribed by it functions with AMP-activated protein kinase in mediating the energy stress response. [provided by RefSeq, Aug 2016]

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