DUSP3 (AK055834) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	VHR
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK055834, the custom clone sequence may differ by one or more nucleotides CACAGAGGTCCCTCATGACCTCCCTTCCCCAACTCCCGAATCCTCTCTTGAGTGTGGACCTCAACACCTT GAGCCCTAGTAAAGGAACTATGCAAATGCAGGCCACTCTCCCCACCACGTCTGTGCCCCGCACTGTCCCC ACAGCCTTCCACACCCTGTGCATAGGCAGCCCTCTCACGTCTTGAGGTCCGAAGCTGGGGTGGGGGTGTC CGTCAGTTATTAGTGGATGGAGATTCCCACAGCAAGGCTGCATTTGAATGATTTCCTTAGGATGAATGGT CCCTACACAAAGAGGCCTTGTGGGCAAACCTGGAGAACCCCCCTAAATCCATAGAGTTTTCAAAATGTGA ATCTTTGGAAGCCTTGAGTTCAGAATCTGCTGCTCTGGAATATTTCCCTTCGATCTTATCTCAGTCACTT CGTTTTTGAGAAGAGTGATGCCTTGGGCATGCTTTTTTTTTTTTTCTTTTTTAGAAAACAGGGAGTTGAA GTCCAACCTATTTAAAAACCCCACCATTTGGAGAATTACAAGGGTTTTGTCCTGAATTGTAGTGTTGGCA AGCCCAAGCCACTCGTGCTAACTGCTTTTTGTCTCGGTTGCTATTCCAAGAACAGAAGGAGGAAGTTGGC CAATTACAGCGTGTGTGCATGGATGTGTGTGGGGGGCGTGCCTCTCAGAAACGCGGCCAGAAGACAAGCA GGGAAGTGAAAGGTCCCAGGCACACACCCTGCCCATTGCAGGTGGCTCTTACAGCTCTCTGGTGCCAGCA CGGGATCCCTGAAGTGACTCAGCCAGGCAGACATGAGACATGGCGGAGTGTCCAAATGGATCCTTTATTG GTGGTAGAGCAAAAAAACCCAAACACGATAAACCTTTCAAAAGACTTTCTAAGGATGATATTGGAATGCA CCAGCCCTCACATGTGTATGCACATTTGCCAGAATATAAGAGTTTTGTTTTAAATACAGTCTTGTTAGGA TTTTACGTTATTGTTATTATGGAAAGTGATTGTGATGCTATTTATCTTCAGGGTCACTCTGGGCAAAGAG AAGGTCCTCAGCCATGCCCCCAGCACCTTGCACATAGGTGTCTGATAAAAGTTTAAGAAATTAAACACTT TTTGAGCACCAAATATATATAGGGCATTGTTCTGGTGGGTGTGTCACGCTCCCAGAAGACTGAATTTATG GTAGGATCACTCGCAAGGCCTTGTGAAGGAGTCTTACCTAAAACAAAAGAAATATCAGGGACTTTTGTTG ACTATTTACAACTCAGTTTTACATTTAAATTCAGGCAGTGTTAATATGCCAAGGTAGGGAATGTGCCTTT TTCAGAGTTGGCCAGGAGCTCCTGGCTGGGACACGGAGAGGCAGGTGTGGCGTAAGGCCTCACTCCCGGC TGTGAAGGTCTCTGATCACACAGAAGCAGCCCTGCCCAGCCTGGTCATTTGCTGTCCGCTTTTCTCTGTG ACCACAGCAGCCCTGAACAACCAGTATGTGTCTTCTTCTCCAGATAGTGAAAAAGGTGTCCAGATAAACC CACCTAAGTGAAATGGCCATCCTCTAAACTGGGTACCTCACTGCACAGCTTCTAGGTAGCCTTCCAACTT AATCTAACTTGAGCCTCACAGTAACCCTGTAAAGTTAGTAGAGCTTGTTCTTGTATTGTGACCTTTTTTA AAAAAAAGGAACCGAGGTTCAGAATGATTAAGGGCCTGGCCCCCAGGGTTGTCCAGCTCCATAAGGTGGA GCTGGGCAAGATTTTGGGTTTGCTGCTCCCTGAAGCTGGATTCTTTCATACGATACTCTTTCTCAAGAAG GGGGCTCCCTGGGATCTCCAGGTGTACTGCACTTACCCTCAATCCAGCCCCGGAGAAGCAAGTGAAAAGG GTGGGTCCCTCATAGGCTAGAATGTGCAGCTCTTTCTCCAGGTGGGATGTAGCACCCCAAAGTAGAGCTT TCTGCTCTGCTCCTGGAAAAGGCTAGGGAGCTGGGGCTGGGGCTCCCCTCCCATGACCAGGCAGTGGTCA CCCCATGGGACAGGCACAGCTACTTACGCGAACACAGCAGGTTGGTGTGGCTGGCTAACTAGGACCTCTC GAAAGTCTCTGTGGGGGCATGAGGGAGAAAAGGCCATTGGGAGAATTACTGCCTTTACTTTGGGACTACT TTTATGCTGATAACTTGGGATTTCTTGATAGTCCTTCACCCCTGAAACCCCGTATTTACTTAACAAGATT TAGCTCTTAGTTCTTCAAGTAAAATTAAAGTCTCTTGTGTAAGAGCCAACACATGCCCAGCTGCGGATGG GAGCTGTTCCTGGACAGCCTTCTACTGCCTGGGAAGTGATGGAACAGGAACTCAGGGTGCCCTTACCCCC TCCCCAGACCTGTTCCCTTTCTTTGACTGACAGAGCACCATCCAGGCAAAATTAGAGCGCCAAATGGTTT TCTTCTCAATCTTAAAGCAGTATACCTTTCCACAGGCTCGTCTGTGTCCCTGCCACTCTGAGTTATCCAG AAACCACCACCTACAAATGAGGGGACTCATCTAGAAGACCTCTAAGGTCCCCTTTTGGCTCTGAGGGGTC TCTAATAATCCCCACTTGGAATTCAGCACCGCAAGGAAATTATGGGTATGTGAGCCATAATATGATGGCC AGCAGGTGGCGCTGCCTTCCACCCATGGTGATGGATGGTTTGGAAAGGGAATGTTGGTGCCTTTTGTGCC ACAAGTTAAGATGCTACTGTTTTAAAGG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK055834 unedited GCCAAAATTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTGGACCTCACA CCTTGAGCCCTAGTAAAGGAACTATGCAAATGCAGGNCCACTCTCCCCACCACGTCTGTG CCCCGCACTGTCCCCACAGCCTTCCACACCCTGTGCATAGGCAGCCCTCTCACGTCTTGA GGTCCGAAGCTGGGGTGGGGGTGTCCGTCAGTTATTAGTGGATGGAGATTCCCACAGCAA GGCTGCATTTGAATGATTTCCTTAGGATGAATGGTCCCTACACAAAGAGGCCTTGTGGGC AAACCTGGAGAACCCTCCTAAATCCATAGAGTTTTCAAAATGTGAATCTTTGGAAGCCTT GAGTTCAGAATCTGCTGCTCTGGAATATTTCCCTTCGATCTTATCTCAGTCACTTCGTTT TTGAGAAGAGTGATGCCTTGGGCATGCTTTTTTTTTTTTCTTTTTTAGAAAACAGGGAGT TGAAGTCCAACCTATTTAAAAACCCCACCATTTGGAGAATTACAAGGGTTTTGTCCTGAA TTGTAGTGTTGGCAAGCCCAAGCCACTCGTGCTAACTGCTTTTTGTCTCGGTTGCTATTC CAAGAACAGAAGGAGGAAGTTGGCCAATTACAGCGTGTGTGCATGGATGTGTGTGGGGGG CGTGCCTCTCAGAAACGCGGCCAGAAGACAAGCAGGGAAGTGAAAGGTCCCAGGCACACA CCCTGCCCATTGCAGGTGGCTCTTACAGCTCTCTGGTGCCCAGCACGGATCCCTGNAGTG ACTCAGCCAGNCAGACATGAGACATGGCGGNAGTGNCCAATGGATCCTTTATNGNNTGTA GAGCANAAAACCCCAACACGAAAACCTTNCAAAGAACTTCTAGGATGATATNGAATGCAC CAGCCTACATGTGTATGCCATTTGCCAGATATAGAANNTTGTTTNATACAGNNCTGTTAG ATTTACGTN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK055834 unedited TGATCGCTGTCCGCAATTANAGTTGAGTTTTTTTTTTTTTTTTTTTTCCTTTAAAACAGT TGCATCTTAACTTGTGGCACAAAAGGCACCAACATTCCCTTTCCAAACCATCCATCACCA TGGGTGGAAGGCAGCGCCACCTGCTGGCCATCATATTATGGCTCACATACCCATAATTTC CTTGCGGTGCTGAATTCCAAGTGGGGATTATTAGAGACCCCTCAGAGCCAAAAGGGGACC TTAGAGGTCTTCTAGATGAGTCCCCTCATTTGTAGGTGGTGGTTTCTGGATAACTCAGAG TGGCAGGGACACAGACGAGCCTGTGGAAAGGTATACTGCTTTAAGATTGAGAAGAAAACC ATTTGGCGCTCTAATTTTGCCTGGATGGTGCTCTGTCAGTCAAAGAAAGGGAACAGGTCT GGGGAGGGGGTAAGGGCACCCTGAGTTCCTGTTCCATCACTTCCCAGGCAGTAGAAGGCT GTCCAGGAACAGCTCCCATCCGCAGCTGGGCATGTGTTGGCTCTTCACAAGAGACTTTAT TTTACTTGAAGACTAAGAGCTAAATCTTGTTAGTAAATACGGGGTTTCAGGGGTGAAGGA CTATCAAGAAATCCCAAAGTATCAGCATAAAAGTAGTCCCCAAGTAAAAGCAGTAATTCT CCCAATGGCTTTTCTCCCTCATGCCCCCACAAAACTTTCAGAAGGCCTAGTTAGCCAGCC CCACCACCTGCTGGGTCCCGTAAGAGCTTGGCCTGGCCAAGGGGTGACACACTGCTGTCA TGGGAGGGAACCCCAACCCCAGTTCCTACCTTTCCAGAACAAGAAAAAAACCTTCTTTTG GGGGCTCTCCCCCCCGGGAAAAAATCGCATTTCTACCTTTAAGGCCCCCCCTTCT
Restriction Sites	NotI-NotI
ACCN	AK055834
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK055834.1
RefSeq Size	2758 bp
RefSeq ORF	2758 bp
Locus ID	1845
Protein Families	Druggable Genome, Phosphatase
Protein Pathways	MAPK signaling pathway
Gene Summary	The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008]

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