ERGIC1 (AK056217) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK056217, the custom clone sequence may differ by one or more nucleotides GATTGACAAATTCTGTAAAGGTTAAGACAGTAACTATTTTAGGCTTTGTGGGCTACATGTGGTCTCTGTA GCACATTCTTCTTTGTTGTTGCTGTTTGTTCATTCGTTTGTTTTGCAACCTTTGGATAATGTAAACCACT CTCTTTTTTTTTTGACAGAGTCTCTCTCTGTAGCCAGGCTGGAGGGCAATTGTACAATCTCGGCTCATTA CAACCTCCACCTCCCGGGTTCAGGCAATTCTCCTGCCTCAGCTTCCCGAGTAGCTGGGACTATAGGTGCG TGCCACCATGCCCAGCTAATATTTTTTTTTTTTTTGTATTTTTAGTAGAGGCAGAGTTTCACCATGTTGG CCAGGATGTTCTCGATCTCCTGACCTTGTGATCCGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGG TGTGAGCTACCGCACCCTGCCAATATAAACTATTCTTAGCTCATGGGCTATGCAAAGCCTTAGGCTGACC CCTGATCCAGACTGTCTTCTGAGACAAGCATTTCTGTGAGCTGCCCCATGTGATAACTGAGTTGTGCAGT AGCTGATGTGGTTTATTGCCTTCCCATCTACTGCATTGTCAGACCAGACTGACTGCACAGGAGCAGGTGG AGGGGGGCTGCATATGCCAACCATTACTCTTCAATAGAGTGTCCTTCATGAGCTCTCTCTCTCTCTCATT ACTGACTACCTCAGTGTAGAAACCCTCATTTTTTTCCCCAGACCTATCCTTGTTACCCAATGTGCCTCTG CTTCCCTGGGTTTGGGATAAACTGGAAGGAGAGAATTTCTGAGTTCTCTTACTTATTTTGTTCCATAACT TCTCCCTCATATCTGTGGTTGGAGACTCCCACCCTTGTCCACCCTTTCCTGCCTCATACTAGTATCATGA GTTTAGGGCTGTCGCTGGAAAGTCTGGCAGCCCAGGCCATAGGATTCCAGTTCAGCCTACGATTTCCTAG GGAAATCACCAAGTTCCAAAGGCTGGTTTTTAAACCTAGAATATCCATGTATCCATTTCCTAATGCAGTT TGGACCTGTTCTAGACAGAGCACTCAGCCTTAGCAAGAAAACCAATGAGGTGCAGAAGATATTCTGTGCC CACAGTTTTTCCCTGCCTTCTGGACCCTCAGCCCCTGGGATGGGCTCCTTGGGATTGCCCATGCAACAAC ACAGCCACAAGACATTCAGCACACCACCTCCTCCTGTGTTTTGAATCTCTAAATGAATTCTGTTCTAAAG CTTCTTCACGTCTCTCTCCATCCTGCTCCCATCTCCACCTCTAATGCTTCTTGGGAGTCTGAAGCTCAGA TTCACATTTTCACATTTTCTCATATAAACGTTGCGGGATAAGAATGGATGATATGTATGTGTCTGTGTGA GTTTGGTTTCTCAACCTGGACTGTGAGGGCTAATTCACAAATCACACCATACATGCGCGTGTGCATGCGC ACACGCACACACACACACACACACACACAGTCCAGCACCCTCATCAGTTCTCTGGTTTAGGTTAGGTCCA GATCTGATGGGACTGGTCAAGAAGGGGATGGTGGGGGCTCCTGAAGCATTTGCCCTTTCTTGCCTCTTGA GACCTTCTTTCCATTGGCAATTCAAAACAGGCTGACATACAGAGGGCTAGCACTCTAATTTGATGTGGAC TCAGCCACTCAGACCTAGGTCTCAGGCTGCAGCTTATCCTAGCTCAGCAAATTGCTTCTTAGAATCTTTA GCTTGATTTCTGTCATTTGAGCCCAGGGATCTACCCACTTACTGAAATCTAAATTTTAAAAAATCAGTGA TGAAAGTAATTTTCAGGATTTCCCTATTTAAAAGCATCCATGAAACAGTTCCTTTATGCCTTCTGTTTAG GATTTCCAGAATTAGGAAGGGAAACCAGGCTTGTGCCTTGGATCCAAAAGCAGCAAAAATACTACACTTG CCAAAAGCAAACTGAGGAATGAGCATTGGCCAGTCCTAGTATGAAGTCAAGAGGTGGACTTTTGTTAAGT TAGCCCTTACCATTTAGTGCTTACTGAATGCCAACAATGCACTTAGAAAAATAAGGGAGACAAGAATTTA CAGACCATACCCTAAACTGGAAGGTACAGGAAAGACACATCTCCTAGAGCTGGGAATGTGTCCCTAACAT TTCAAGAAGAAGCAGAAAGTAGGAGAGATCACGATCTCAGTCTTTTTAGGATTCCAACACAAAATGGAAG GGAGCCCTCTGTGGGGTGATATTACAAAGGGTTTTTCTTGTCTTTCTTTAAAAAAAAAAGGCATTCCTTA CTGGCTTGCTACCAGCAATTGAACTGTATTCTTTATATAGCTAGAGCTGTTTGTGTCTTCTTAAAATAAA CTTTTTCTGTTAAAATC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK056217 unedited TCCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA ATTCGGCACGAGGCTTCCTCTCGTTTCTCTTTTCGAGGATGCTTAGAGGCTTCTCACCAG AGATTGGTGAGGTTTCCCCCATCATCTAGATGGGAGATTGACAAATTCTGTAAAGGTTAA GACAGTAACTATTTTAGGTTTTGTGGGCTACATGTGGTCTCTGTAGCACATTCTTCTTTG TTGTTGCTGTTTGTTCATTCGTTTGTTTTGCAACCTTTGGATAATGTAAACCACTCTCTT TTTTTTGACAGAGTCTCTCTCTGTAGCCAGGCTGGAGGGCAATTGTACAATCTCGGCTCA TTACAACCTCCACCTCCCGGGTTCAGGCAATTCTCCTGCCTCAGCTTCCCGAGTAGCTGG GACTATAGGTGCGTGCCACCATGCCCAGCTAATATTTTTTTTTTTTTTGTATTTTTAGTA GAGGCAGAGTTTCACCATGTTGGCCAGGATGTTCTCGATCTCCTGACCTTGTGATCCGCC CACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCTACCGCACCCTGCCAATATA AACTATTCTTAGCTCATGGGCTATGCAAAGCCTTAGGCTGACCCCTGATCCAGACTGTCT TCTGAGACAAGCATTTCTGTGAGCTGCCCCATGTGAAACTGNAGTTGTGCAGTAGCTGAT GTGGTTTATTGCCTTCCCATCTACTGCATTGTCAGACCAGACTGACTGCCANGAGCAGGT GGAGGGGGGCTGCATATGCCACCATTACTCTTCATAGAGTGCCTTCATGAGCTCTCTCTC TCTCTCATACTGACTACCTCAGTGTAGAACCCTCTTTTTTNCCCAGACTNTCCTTGTTAC CNAAGTGCCTCTGCTCCCN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK056217 unedited ACCTTTCCTTTTGATTTTAACAGATTTATTTATTTTAAGAAGACACAAACAGCTCTAGCT ATATAAAGAATACAGTTCAATTGCTGGTAGCAAGCCAGTAAGGAATGCCTTTTTTTTTTA AAGAAAGACAAGAAAAACCCTTTGTAATATCACCCCACAGAGGGCTCCCTTTTCTTTGTT GTTGGAATCCTAAAAAGACTGAGGTCGGGATCTCTCCTACTTTCTGCTTCTTCTTGAAAT GTTAGGGACACATTCCCAGCTCTAGGAGATGTGTCTTTCCTGTACCTTCCAGTTTAAGGT ATGGTCTGTAAATTCTTGTCTCCCTTATTTTTCTAAGTGCATTGTTGGCATTCAGTAAGC ACTAAATGGTAAGGGCTAACTTAACAAAAGTCCACCTCTTGACTTCATACTAGGACTGGC CAATGCTCATTCCTCAGTTTGCTTTTGGCAAGTGTAGTATTTTTGCTGCTTTTGGATCCA AGGCACAAGCCTGGTTTCCCTTCCTAATTCTGGAAATCCTAAACAGAAGGCATAAAGGAA CTGTTTCATGGATGCTTTTAAATAGGGAAATCCTGAAAATTACTTTCATCACTGATTNTT TAAAATTTAGATTTCAGTAAGTGGGTAGATCCCTGGGCTCAAATGACAGAAATCAAGCTA AAGATTCTAAAGAAGCATTTGCTGAGCTAGGATAAGCTGCAGCCTGAGACCTAAGTCTGA GTGGCTGAGTCCACATCAAATTANAGTGCTAGCCCTCTGTATGTCAGCCTGTTTTGAATG GCCAAGGAAAGAAGTCTCCAGAGGCAAGAAGGGCAAATGCTTCAAGAGCCCCCACCT
Restriction Sites	NotI-NotI
ACCN	AK056217
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK056217.1
RefSeq Size	2397 bp
RefSeq ORF	2397 bp
Locus ID	57222
Protein Families	Transmembrane
Gene Summary	This gene encodes a cycling membrane protein which is an endoplasmic reticulum-golgi intermediate compartment (ERGIC) protein which interacts with other members of this protein family to increase their turnover. [provided by RefSeq, Jul 2008]

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