ST6GALNAC3 (AK091215) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PRO7177; SIAT7C; ST6GALNACIII; STY
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >SC104409 representing AK091215. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCTGCATCCTGAAGAGAAAGTCTGTGATTGCTGTGAGCTTCATAGCAGCGTTCCTTTTCCTGCTG GTTGTGCGTCTTGTAAATGAAGTGAATTTCCCATTGCTACTAAACTGCTTTGGACAACCTGGTACAAAG TGGATACCATTCTCCTACACATACAGGCGGCCCCTTCGAACTCACTATGGATACATAAATGTGAAGACA CAAGAGCCTTTGCAACTGGACTGTGACCTTTGTGCCATAGTGTCAAACTCAGGTCAGATGGTTGGCCAG AAGGTGGGAAATGAGATAGATCGATCCTCCTGCATTTGGAGAATGAACAATGCCCCCACCAAAGGTTAT GAAGAAGATGTCGGCCGCATGACCATGATTCGAGTTGTGTCCCATACCAGCGTTCCTCTTTTGCTAAAA AACCCTGATTATTTTTTCAAGGAAGCGAATACTACTATTTATGTTATTTGGGGACCTTTCCGCAATATG AGGAAAGATGGCAATGGCATCGTTTACAACATGTTGAAAAAGACAGTTGGTATCTATCCGAATGCCCAA ATATACGTGACCACAGAGAAGCGCATGAGTTACTGTGATGGAGTTTTTAAGAAGAAAACTGGGAAGGAC AGAGTCCAGTCTGGCTCATATCTCAGCACAGGGTGGTTTACCTTCCTTCTGGCCATGGACGCCTGTTAT GGCATTCACGTCTACGGGATGATAAATGACACCTACTGCAAGACAGAAGGGTATAGAAAAGTCCCCTAC CATTATTATGAACAAGGAAGAGATGAGTGTGATGAATATTTTCTTCATGAACATGCCCCATATGGGGGT CATAGGTTTATCACTGAAAAGAAAGTGTTTGCTAAATGGGCCAAGAAGCACAGGATAATATTTACACAT CCAAACTGGACATTGTCTTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091215 unedited CCTCATTACCCCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG CCGCGAATTGGCACGAGGCTGGAGAGGTCCTGCCGTGGTACCAGCCTCCAGCCTGCCCCC AGGACTGCCCCTGACCCAGGCGCGCCCGCTGCTCGGTGGCAGGAGGGCCGGCGGAGCGCC ATGGCCTGCATCCTGAAGAGAAAGTCTGTGATTGCTGTGAGCTTCATAGCAGCGTTCCTT TTCCTGCTGGTTGTGCGTCTTGTAAATGAAGTGAATTTCCCATTGCTACTAAACTGCTTT GGACAACCTGGTACAAAGTGGATACCATTCTCCTACACATACAGGCGGCCCCTTCGAACT CACTATGGATACATAAATGTGAAGACACAAGAGCCTTTGCAACTGGACTGTGACCTTTGT GCCATAGTGTCAAACTCAGGTCAGATGGTTGGCCAGAAGGTGGGAAATGAGATAGATCGA TCCTCCTGCATTTGGAGAATGAACAATGCCCCCACCAAAGGTTATGAAGAAGATGTCGGC CGCATGACCATGATTCGAGTTGTGTCCCATACCAGCGTTCCTCTTTTGCTAAAAAACCCT GATTATTTTTTCAAGGAAGCGAATACTACTATTTATGTTATTTGGGGACCTTTCCGCATA TGANGAAAGATGGCAATGGCATTCGTTACAACATGGTTGAAAAGACAGTTGGTATCTATC CCGATGCCCCAATATACGTGACCACAGAGAGCGCATGAGTTACTGTGATGGAGGTTTTAG AAGAAAACTGGAGGACAGATCCAGTCTGGCTATATCTCAGCACAGGTGGGTTACCTTCCT TCTGCCATGGACGCCTGTATGCATCACGTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091215 unedited NNNGGGGGTTCCACTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTT TTTTTTTTTTTGATTAATCAAGGAATTTCTTTTTATTTTAAAGTGAAACAACAGGGGGGA AAAACAGTCTGTATGGCAATTTTAACATTTGTCTCTGTTTATTTTTCTTTGAGTGGGCAT TTCTATCTATCATTAGCTCAAAAAAACTTAACATTCATAGAATTAAAAATTGCATAAGGG AACAACAAAAGAATAACAACTCCTTCCAACGATAGAACTGGGGCTTAATAATGTTTATGG GTGTGAATATTGAGGTGTCAAAATTGCAGAAAAGAGTAGGCAAAAGGCCTTTATCCAAAA TGGCACACTTTAATGATAGCTCACCATAATACTTAGCACTAGTAAAGACCCACAAGTGTT TCTGAAGTGAAATGTATCATTACTTCCAGTTTCCCAAACATTCTATAAGTTGAGCCTGGC TTTTGTCTTTTGAATCATTCTGCCAGGATGTTTTTTTGAATTTCTCAACTACCCCTTTGC GCAACAAAACCCTTTTCCTATTTAATGAGGCACCAGTATTTTAAAAATACCATTGTCTCC ACCCTATCAAAGGACAATATTTTTTCTCTGCCCTTATATCCCCCCTGGGGCCCCGGTTTT AGATCCTTTTCCCCCCCAAATTCAACCCCCCCTTGTTACTTTCCCCCTTTCGAAAAACCT TCTCAAAATTTCTTTCTTTTCTCAAGGGGCCCTATCCCCTCAGCCCCCAACCAATCCTTG GGGATCCCTTTCTCCGGTATCCAGCTCAATGACATTTCGCACAACCACTACCCGGTTAAC AATTTCACATAAACTTCCCCCTCCCCCACCCCTACC
Restriction Sites	NotI-NotI
ACCN	AK091215
Insert Size	918 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091215.1
RefSeq Size	3207 bp
RefSeq ORF	918 bp
Locus ID	256435
Domains	Glyco_transf_29
Protein Families	Transmembrane
Protein Pathways	Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
MW	35.4 kDa
Gene Summary	ST6GALNAC3 belongs to a family of sialyltransferases that transfer sialic acids from CMP-sialic acid to terminal positions of carbohydrate groups in glycoproteins and glycolipids (Lee et al., 1999 [PubMed 10207017]).[supplied by OMIM, Mar 2008]

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